Lentic and effluent water of Delhi-NCR: a reservoir of multidrug-resistant bacteria harbouring blaCTX-M, blaTEM and blaSHV type ESBL genes

Antimicrobial resistance is not restricted to clinics but also spreading fast in the aquatic environment. This study focused on the prevalence and diversity of extended-spectrum β-lactamase (ESBL) genes among bacteria from lentic and effluent water in Delhi-NCR, India. Phenotypic screening of 436 mo...

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Autores principales:	Asghar Ali, Insha Sultan, Aftab Hossain Mondal, Mohammad Tahir Siddiqui, Firdoos Ahmad Gogry, Qazi Mohd. Rizwanul Haq
Formato:	article
Lenguaje:	EN
Publicado:	IWA Publishing 2021
Materias:	amr aquatic environment blactx-m blashv blatem esbls Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/5828219aaa3f412abba0a11f6201ed8e
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spelling	oai:doaj.org-article:5828219aaa3f412abba0a11f6201ed8e2021-11-06T06:28:22ZLentic and effluent water of Delhi-NCR: a reservoir of multidrug-resistant bacteria harbouring blaCTX-M, blaTEM and blaSHV type ESBL genes1477-89201996-782910.2166/wh.2021.085https://doaj.org/article/5828219aaa3f412abba0a11f6201ed8e2021-08-01T00:00:00Zhttp://jwh.iwaponline.com/content/19/4/592https://doaj.org/toc/1477-8920https://doaj.org/toc/1996-7829Antimicrobial resistance is not restricted to clinics but also spreading fast in the aquatic environment. This study focused on the prevalence and diversity of extended-spectrum β-lactamase (ESBL) genes among bacteria from lentic and effluent water in Delhi-NCR, India. Phenotypic screening of 436 morphologically distinct bacterial isolates collected from diverse sites revealed that 106 (∼24%) isolates were ESBL positive. Antibiotic profiling showed that 42, 60, 78 and 59% ESBL producing isolates collected from Ghazipur slaughterhouse, Lodhi garden pond, Hauz Khas lake and Jasola wastewater treatment plant, respectively, were multidrug-resistant (MDR). The multiple antibiotic resistance (MAR) index varied from 0.20 to 0.32 among selected locations. The prevalence of ESBL gene variants blaSHV, blaTEM and blaCTX-M were found to be 17.64, 35.29 and 64%, respectively. Furthermore, the analysis of obtained gene sequences showed three variants of blaCTX-M (15, 152 and 205) and two variants of blaTEM (TEM-1 and TEM-116) among ESBL producers. The co-existence of 2–3 gene variants was recorded among 48% ESBL positive isolates. New reports from this study include the blaCTX-M gene in Acinetobacter lwoffii, Enterobacter ludwigii, Exiguobacterium mexicanum and Aeromonas caviae. Furthermore, the identification of blaTEM and blaSHV in an environmental isolate of A. caviae is a new report from India. HIGHLIGHTS Occurrence of ESBL genes from Exiguobacterium mexicanum was first reported.; blaCTX-M gene from environmental isolate of Aeromonas caviae is the first report.; Co-resistance of β-lactam and non-β-lactam classes of antibiotics was observed among a high proportion of ESBL positive bacterial isolates.; This study emphasizes the need for more comprehensive genetic research of diverse microorganisms from environmental settings.;Asghar AliInsha SultanAftab Hossain MondalMohammad Tahir SiddiquiFirdoos Ahmad GogryQazi Mohd. Rizwanul HaqIWA Publishingarticleamraquatic environmentblactx-mblashvblatemesblsPublic aspects of medicineRA1-1270ENJournal of Water and Health, Vol 19, Iss 4, Pp 592-603 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	amr aquatic environment blactx-m blashv blatem esbls Public aspects of medicine RA1-1270
spellingShingle	amr aquatic environment blactx-m blashv blatem esbls Public aspects of medicine RA1-1270 Asghar Ali Insha Sultan Aftab Hossain Mondal Mohammad Tahir Siddiqui Firdoos Ahmad Gogry Qazi Mohd. Rizwanul Haq Lentic and effluent water of Delhi-NCR: a reservoir of multidrug-resistant bacteria harbouring blaCTX-M, blaTEM and blaSHV type ESBL genes
description	Antimicrobial resistance is not restricted to clinics but also spreading fast in the aquatic environment. This study focused on the prevalence and diversity of extended-spectrum β-lactamase (ESBL) genes among bacteria from lentic and effluent water in Delhi-NCR, India. Phenotypic screening of 436 morphologically distinct bacterial isolates collected from diverse sites revealed that 106 (∼24%) isolates were ESBL positive. Antibiotic profiling showed that 42, 60, 78 and 59% ESBL producing isolates collected from Ghazipur slaughterhouse, Lodhi garden pond, Hauz Khas lake and Jasola wastewater treatment plant, respectively, were multidrug-resistant (MDR). The multiple antibiotic resistance (MAR) index varied from 0.20 to 0.32 among selected locations. The prevalence of ESBL gene variants blaSHV, blaTEM and blaCTX-M were found to be 17.64, 35.29 and 64%, respectively. Furthermore, the analysis of obtained gene sequences showed three variants of blaCTX-M (15, 152 and 205) and two variants of blaTEM (TEM-1 and TEM-116) among ESBL producers. The co-existence of 2–3 gene variants was recorded among 48% ESBL positive isolates. New reports from this study include the blaCTX-M gene in Acinetobacter lwoffii, Enterobacter ludwigii, Exiguobacterium mexicanum and Aeromonas caviae. Furthermore, the identification of blaTEM and blaSHV in an environmental isolate of A. caviae is a new report from India. HIGHLIGHTS Occurrence of ESBL genes from Exiguobacterium mexicanum was first reported.; blaCTX-M gene from environmental isolate of Aeromonas caviae is the first report.; Co-resistance of β-lactam and non-β-lactam classes of antibiotics was observed among a high proportion of ESBL positive bacterial isolates.; This study emphasizes the need for more comprehensive genetic research of diverse microorganisms from environmental settings.;
format	article
author	Asghar Ali Insha Sultan Aftab Hossain Mondal Mohammad Tahir Siddiqui Firdoos Ahmad Gogry Qazi Mohd. Rizwanul Haq
author_facet	Asghar Ali Insha Sultan Aftab Hossain Mondal Mohammad Tahir Siddiqui Firdoos Ahmad Gogry Qazi Mohd. Rizwanul Haq
author_sort	Asghar Ali
title	Lentic and effluent water of Delhi-NCR: a reservoir of multidrug-resistant bacteria harbouring blaCTX-M, blaTEM and blaSHV type ESBL genes
title_short	Lentic and effluent water of Delhi-NCR: a reservoir of multidrug-resistant bacteria harbouring blaCTX-M, blaTEM and blaSHV type ESBL genes
title_full	Lentic and effluent water of Delhi-NCR: a reservoir of multidrug-resistant bacteria harbouring blaCTX-M, blaTEM and blaSHV type ESBL genes
title_fullStr	Lentic and effluent water of Delhi-NCR: a reservoir of multidrug-resistant bacteria harbouring blaCTX-M, blaTEM and blaSHV type ESBL genes
title_full_unstemmed	Lentic and effluent water of Delhi-NCR: a reservoir of multidrug-resistant bacteria harbouring blaCTX-M, blaTEM and blaSHV type ESBL genes
title_sort	lentic and effluent water of delhi-ncr: a reservoir of multidrug-resistant bacteria harbouring blactx-m, blatem and blashv type esbl genes
publisher	IWA Publishing
publishDate	2021
url	https://doaj.org/article/5828219aaa3f412abba0a11f6201ed8e
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Lentic and effluent water of Delhi-NCR: a reservoir of multidrug-resistant bacteria harbouring blaCTX-M, blaTEM and blaSHV type ESBL genes

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