EFFECTS OF DEHYDROEPIANDROSTERONE SULFATE UPON PHENOTYPE AND IN VITRO FUNCTIONS OF DENDRITIC CELLS

Abstract. The study deals with evaluation of dehydroepiandrosterone sulfate (DHEAS) effects upon phenotype and functions of dendritic cells (DCs), generated from blood monocytes of pregnant women by means of GM-CSF and IFN-alpha. It was shown, that initial supplementation with DHEAS (10-6 M, day 1 o...

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Autores principales:	N. V. Seledzova, N. A. Khonina, M. A. Tikhonova, A. A. Ostanin, N. M. Passman, E. R. Chernykh
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	dehydroepiandrosterone sulfate dendritic cells nk-cells hyperandrogenia Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/58355f1ce1d446f0b07adaeae0b2efb3
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spelling	oai:doaj.org-article:58355f1ce1d446f0b07adaeae0b2efb32021-11-18T08:03:38ZEFFECTS OF DEHYDROEPIANDROSTERONE SULFATE UPON PHENOTYPE AND IN VITRO FUNCTIONS OF DENDRITIC CELLS1563-06252313-741X10.15789/1563-0625-2007-6-589-596https://doaj.org/article/58355f1ce1d446f0b07adaeae0b2efb32014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/245https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. The study deals with evaluation of dehydroepiandrosterone sulfate (DHEAS) effects upon phenotype and functions of dendritic cells (DCs), generated from blood monocytes of pregnant women by means of GM-CSF and IFN-alpha. It was shown, that initial supplementation with DHEAS (10-6 M, day 1 of culture) did not influence the pattern of DC subsets. Meanwhile, addition of DHEAS at the stage of DC maturation (last day of culture) is accompanied by a significant increase in mature CD83+ cells and activated DCs (CD25+), like as their precursors (CD1a+). Furthermore, the DCs generated in presence of DHEAS were characterized by marked allostimulating activity and decreased ability to downregulate the numbers of CD56+CD16+ NK cells. Hence, DHEAS promotes generation, maturation, and allostimulating activity of DCs, along with decreased negative regulation towards CD56+CD16+NK cell amounts. DHEAS-mediated changes in DCs’ phenotype and functioning are discussed as a possible mechanism of disturbed immunological tolerance to fetal antigens in pregnant women with suprarenal hyperandrogenia.N. V. SeledzovaN. A. KhoninaM. A. TikhonovaA. A. OstaninN. M. PassmanE. R. ChernykhSPb RAACIarticledehydroepiandrosterone sulfatedendritic cellsnk-cellshyperandrogeniaImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 9, Iss 6, Pp 589-596 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	dehydroepiandrosterone sulfate dendritic cells nk-cells hyperandrogenia Immunologic diseases. Allergy RC581-607
spellingShingle	dehydroepiandrosterone sulfate dendritic cells nk-cells hyperandrogenia Immunologic diseases. Allergy RC581-607 N. V. Seledzova N. A. Khonina M. A. Tikhonova A. A. Ostanin N. M. Passman E. R. Chernykh EFFECTS OF DEHYDROEPIANDROSTERONE SULFATE UPON PHENOTYPE AND IN VITRO FUNCTIONS OF DENDRITIC CELLS
description	Abstract. The study deals with evaluation of dehydroepiandrosterone sulfate (DHEAS) effects upon phenotype and functions of dendritic cells (DCs), generated from blood monocytes of pregnant women by means of GM-CSF and IFN-alpha. It was shown, that initial supplementation with DHEAS (10-6 M, day 1 of culture) did not influence the pattern of DC subsets. Meanwhile, addition of DHEAS at the stage of DC maturation (last day of culture) is accompanied by a significant increase in mature CD83+ cells and activated DCs (CD25+), like as their precursors (CD1a+). Furthermore, the DCs generated in presence of DHEAS were characterized by marked allostimulating activity and decreased ability to downregulate the numbers of CD56+CD16+ NK cells. Hence, DHEAS promotes generation, maturation, and allostimulating activity of DCs, along with decreased negative regulation towards CD56+CD16+NK cell amounts. DHEAS-mediated changes in DCs’ phenotype and functioning are discussed as a possible mechanism of disturbed immunological tolerance to fetal antigens in pregnant women with suprarenal hyperandrogenia.
format	article
author	N. V. Seledzova N. A. Khonina M. A. Tikhonova A. A. Ostanin N. M. Passman E. R. Chernykh
author_facet	N. V. Seledzova N. A. Khonina M. A. Tikhonova A. A. Ostanin N. M. Passman E. R. Chernykh
author_sort	N. V. Seledzova
title	EFFECTS OF DEHYDROEPIANDROSTERONE SULFATE UPON PHENOTYPE AND IN VITRO FUNCTIONS OF DENDRITIC CELLS
title_short	EFFECTS OF DEHYDROEPIANDROSTERONE SULFATE UPON PHENOTYPE AND IN VITRO FUNCTIONS OF DENDRITIC CELLS
title_full	EFFECTS OF DEHYDROEPIANDROSTERONE SULFATE UPON PHENOTYPE AND IN VITRO FUNCTIONS OF DENDRITIC CELLS
title_fullStr	EFFECTS OF DEHYDROEPIANDROSTERONE SULFATE UPON PHENOTYPE AND IN VITRO FUNCTIONS OF DENDRITIC CELLS
title_full_unstemmed	EFFECTS OF DEHYDROEPIANDROSTERONE SULFATE UPON PHENOTYPE AND IN VITRO FUNCTIONS OF DENDRITIC CELLS
title_sort	effects of dehydroepiandrosterone sulfate upon phenotype and in vitro functions of dendritic cells
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/58355f1ce1d446f0b07adaeae0b2efb3
work_keys_str_mv	AT nvseledzova effectsofdehydroepiandrosteronesulfateuponphenotypeandinvitrofunctionsofdendriticcells AT nakhonina effectsofdehydroepiandrosteronesulfateuponphenotypeandinvitrofunctionsofdendriticcells AT matikhonova effectsofdehydroepiandrosteronesulfateuponphenotypeandinvitrofunctionsofdendriticcells AT aaostanin effectsofdehydroepiandrosteronesulfateuponphenotypeandinvitrofunctionsofdendriticcells AT nmpassman effectsofdehydroepiandrosteronesulfateuponphenotypeandinvitrofunctionsofdendriticcells AT erchernykh effectsofdehydroepiandrosteronesulfateuponphenotypeandinvitrofunctionsofdendriticcells
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EFFECTS OF DEHYDROEPIANDROSTERONE SULFATE UPON PHENOTYPE AND IN VITRO FUNCTIONS OF DENDRITIC CELLS

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