Identification and mapping of quantitative trait loci for resistance to Liriomyza trifolii in romaine lettuce cultivar ‘Valmaine’

Abstract Liriomyza trifolii (Diptera: Agromyzidae) is a leafminer that causes ruinous damage to many leafy vegetables including lettuce (Lactuca sativa L.) by stippling and tunneling the leaves. In this study, a population of 125 F3 families was developed from the intraspecific cross of ‘Valmaine’ (...

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Autores principales:	Ramkrishna Kandel, Huangjun Lu, Germán V. Sandoya
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/583d9974607344698846d2c0ea710ad0
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spelling	oai:doaj.org-article:583d9974607344698846d2c0ea710ad02021-12-02T14:12:41ZIdentification and mapping of quantitative trait loci for resistance to Liriomyza trifolii in romaine lettuce cultivar ‘Valmaine’10.1038/s41598-020-80050-52045-2322https://doaj.org/article/583d9974607344698846d2c0ea710ad02021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80050-5https://doaj.org/toc/2045-2322Abstract Liriomyza trifolii (Diptera: Agromyzidae) is a leafminer that causes ruinous damage to many leafy vegetables including lettuce (Lactuca sativa L.) by stippling and tunneling the leaves. In this study, a population of 125 F3 families was developed from the intraspecific cross of ‘Valmaine’ (resistant) and ‘Okeechobee’ (susceptible) romaine cultivars for inheritance analysis and molecular mapping of the resistance loci controlling stippling damage. The experiments were conducted in an insectarium (controlled environment). Stippling damage proved to be heritable because the broad-sense heritability (H 2) was 0.58. A segregation analysis suggested that a single dominant allele, Sd1 locus, controls resistance against L. trifolii. Furthermore, a quantitative trait loci (QTL) analysis identified one novel QTL, named Stippling on LG5 (qSTP5), flanked by two SNPs that were mapped to a 5.2 cM (8.5 Mb region) interval, explaining over 13% of the total phenotypic variance. Desirable allele for resistance to L. trifolii was derived from resistant cultivar Valmaine. Identification of SNPs closely linked to the QTL responsible for L. trifolii resistance should facilitate plant breeders to develop resistant romaine lettuce cultivars.Ramkrishna KandelHuangjun LuGermán V. SandoyaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ramkrishna Kandel Huangjun Lu Germán V. Sandoya Identification and mapping of quantitative trait loci for resistance to Liriomyza trifolii in romaine lettuce cultivar ‘Valmaine’
description	Abstract Liriomyza trifolii (Diptera: Agromyzidae) is a leafminer that causes ruinous damage to many leafy vegetables including lettuce (Lactuca sativa L.) by stippling and tunneling the leaves. In this study, a population of 125 F3 families was developed from the intraspecific cross of ‘Valmaine’ (resistant) and ‘Okeechobee’ (susceptible) romaine cultivars for inheritance analysis and molecular mapping of the resistance loci controlling stippling damage. The experiments were conducted in an insectarium (controlled environment). Stippling damage proved to be heritable because the broad-sense heritability (H 2) was 0.58. A segregation analysis suggested that a single dominant allele, Sd1 locus, controls resistance against L. trifolii. Furthermore, a quantitative trait loci (QTL) analysis identified one novel QTL, named Stippling on LG5 (qSTP5), flanked by two SNPs that were mapped to a 5.2 cM (8.5 Mb region) interval, explaining over 13% of the total phenotypic variance. Desirable allele for resistance to L. trifolii was derived from resistant cultivar Valmaine. Identification of SNPs closely linked to the QTL responsible for L. trifolii resistance should facilitate plant breeders to develop resistant romaine lettuce cultivars.
format	article
author	Ramkrishna Kandel Huangjun Lu Germán V. Sandoya
author_facet	Ramkrishna Kandel Huangjun Lu Germán V. Sandoya
author_sort	Ramkrishna Kandel
title	Identification and mapping of quantitative trait loci for resistance to Liriomyza trifolii in romaine lettuce cultivar ‘Valmaine’
title_short	Identification and mapping of quantitative trait loci for resistance to Liriomyza trifolii in romaine lettuce cultivar ‘Valmaine’
title_full	Identification and mapping of quantitative trait loci for resistance to Liriomyza trifolii in romaine lettuce cultivar ‘Valmaine’
title_fullStr	Identification and mapping of quantitative trait loci for resistance to Liriomyza trifolii in romaine lettuce cultivar ‘Valmaine’
title_full_unstemmed	Identification and mapping of quantitative trait loci for resistance to Liriomyza trifolii in romaine lettuce cultivar ‘Valmaine’
title_sort	identification and mapping of quantitative trait loci for resistance to liriomyza trifolii in romaine lettuce cultivar ‘valmaine’
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/583d9974607344698846d2c0ea710ad0
work_keys_str_mv	AT ramkrishnakandel identificationandmappingofquantitativetraitlociforresistancetoliriomyzatrifoliiinromainelettucecultivarvalmaine AT huangjunlu identificationandmappingofquantitativetraitlociforresistancetoliriomyzatrifoliiinromainelettucecultivarvalmaine AT germanvsandoya identificationandmappingofquantitativetraitlociforresistancetoliriomyzatrifoliiinromainelettucecultivarvalmaine
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Identification and mapping of quantitative trait loci for resistance to Liriomyza trifolii in romaine lettuce cultivar ‘Valmaine’

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