Galangin ameliorates severe acute pancreatitis in mice by activating the nuclear factor E2-related factor 2/heme oxygenase 1 pathway

Acute pancreatitis (AP) is a common serious acute condition of the digestive system that remains a clinical challenge. Severe acute pancreatitis (SAP) in particular is characterized by high morbidity and mortality. The present study was designed to investigate the protective effect of Galangin (Gal)...

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Autores principales:	Yao-Dong Song, Yan-Yan Liu, De-Jian Li, Shu-Jun Yang, Qiao-Fang Wang, Yan-Na Liu, Meng-Ke Li, Chao-Peng Mei, Hu-Ning Cui, San-Yang Chen, Chang-Ju Zhu
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Galangin Acute pancreatitis Inflammation Oxidative stress Nuclear factor E2-related factor 2 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/584c7aab2eb54855836717933a1e927b
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spelling	oai:doaj.org-article:584c7aab2eb54855836717933a1e927b2021-11-14T04:29:19ZGalangin ameliorates severe acute pancreatitis in mice by activating the nuclear factor E2-related factor 2/heme oxygenase 1 pathway0753-332210.1016/j.biopha.2021.112293https://doaj.org/article/584c7aab2eb54855836717933a1e927b2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0753332221010775https://doaj.org/toc/0753-3322Acute pancreatitis (AP) is a common serious acute condition of the digestive system that remains a clinical challenge. Severe acute pancreatitis (SAP) in particular is characterized by high morbidity and mortality. The present study was designed to investigate the protective effect of Galangin (Gal), a natural flavonol obtained from lesser galangal, on L-arginine-induced SAP in mice and in AR42J cells. Amylase and lipase activities were measured and the histopathology of the pancreas, lung, and kidney was evaluated. Inflammation and oxidative stress were assessed using ELISA, western blotting, RT-PCR, and immunohistochemistry. Gal was shown to reduce proinflammatory cytokine production and reactive oxygen species (ROS) generation in vivo and in vitro. L-arginine treatment reduced the expression of components of the nuclear factor E2-related factor 2 (Nrf2) signaling pathway and the downstream protein heme oxygenase-1 (HO-1) in mice, whereas Gal increased their expression. Furthermore, the Nrf2/HO-1 pathway inhibitor brusatol prevented the anti-inflammatory and antioxidant effects of Gal in mice with SAP. Taken together, our results imply that Gal has protective effects in L-arginine-induced SAP that are induced by the upregulation of the Nrf2/HO-1 pathway, which has anti-inflammatory and antioxidant effects. Thus, Gal may represent a promising treatment for SAP.Yao-Dong SongYan-Yan LiuDe-Jian LiShu-Jun YangQiao-Fang WangYan-Na LiuMeng-Ke LiChao-Peng MeiHu-Ning CuiSan-Yang ChenChang-Ju ZhuElsevierarticleGalanginAcute pancreatitisInflammationOxidative stressNuclear factor E2-related factor 2Therapeutics. PharmacologyRM1-950ENBiomedicine & Pharmacotherapy, Vol 144, Iss , Pp 112293- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Galangin Acute pancreatitis Inflammation Oxidative stress Nuclear factor E2-related factor 2 Therapeutics. Pharmacology RM1-950
spellingShingle	Galangin Acute pancreatitis Inflammation Oxidative stress Nuclear factor E2-related factor 2 Therapeutics. Pharmacology RM1-950 Yao-Dong Song Yan-Yan Liu De-Jian Li Shu-Jun Yang Qiao-Fang Wang Yan-Na Liu Meng-Ke Li Chao-Peng Mei Hu-Ning Cui San-Yang Chen Chang-Ju Zhu Galangin ameliorates severe acute pancreatitis in mice by activating the nuclear factor E2-related factor 2/heme oxygenase 1 pathway
description	Acute pancreatitis (AP) is a common serious acute condition of the digestive system that remains a clinical challenge. Severe acute pancreatitis (SAP) in particular is characterized by high morbidity and mortality. The present study was designed to investigate the protective effect of Galangin (Gal), a natural flavonol obtained from lesser galangal, on L-arginine-induced SAP in mice and in AR42J cells. Amylase and lipase activities were measured and the histopathology of the pancreas, lung, and kidney was evaluated. Inflammation and oxidative stress were assessed using ELISA, western blotting, RT-PCR, and immunohistochemistry. Gal was shown to reduce proinflammatory cytokine production and reactive oxygen species (ROS) generation in vivo and in vitro. L-arginine treatment reduced the expression of components of the nuclear factor E2-related factor 2 (Nrf2) signaling pathway and the downstream protein heme oxygenase-1 (HO-1) in mice, whereas Gal increased their expression. Furthermore, the Nrf2/HO-1 pathway inhibitor brusatol prevented the anti-inflammatory and antioxidant effects of Gal in mice with SAP. Taken together, our results imply that Gal has protective effects in L-arginine-induced SAP that are induced by the upregulation of the Nrf2/HO-1 pathway, which has anti-inflammatory and antioxidant effects. Thus, Gal may represent a promising treatment for SAP.
format	article
author	Yao-Dong Song Yan-Yan Liu De-Jian Li Shu-Jun Yang Qiao-Fang Wang Yan-Na Liu Meng-Ke Li Chao-Peng Mei Hu-Ning Cui San-Yang Chen Chang-Ju Zhu
author_facet	Yao-Dong Song Yan-Yan Liu De-Jian Li Shu-Jun Yang Qiao-Fang Wang Yan-Na Liu Meng-Ke Li Chao-Peng Mei Hu-Ning Cui San-Yang Chen Chang-Ju Zhu
author_sort	Yao-Dong Song
title	Galangin ameliorates severe acute pancreatitis in mice by activating the nuclear factor E2-related factor 2/heme oxygenase 1 pathway
title_short	Galangin ameliorates severe acute pancreatitis in mice by activating the nuclear factor E2-related factor 2/heme oxygenase 1 pathway
title_full	Galangin ameliorates severe acute pancreatitis in mice by activating the nuclear factor E2-related factor 2/heme oxygenase 1 pathway
title_fullStr	Galangin ameliorates severe acute pancreatitis in mice by activating the nuclear factor E2-related factor 2/heme oxygenase 1 pathway
title_full_unstemmed	Galangin ameliorates severe acute pancreatitis in mice by activating the nuclear factor E2-related factor 2/heme oxygenase 1 pathway
title_sort	galangin ameliorates severe acute pancreatitis in mice by activating the nuclear factor e2-related factor 2/heme oxygenase 1 pathway
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/584c7aab2eb54855836717933a1e927b
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Galangin ameliorates severe acute pancreatitis in mice by activating the nuclear factor E2-related factor 2/heme oxygenase 1 pathway

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