Delivery of sodium morrhuate to hemangioma endothelial cells using immunoliposomes conjugated with anti-VEGFR2/KDR antibody

Xiaoli Li,1 Xiaoyong Ren,2 Jianmin Liang,2 Weijun Ma,2 Zhenghui Wang,2 Zhuangqun Yang3 1Department of Dermatology, The Second Affiliated Hospital, Xi’an Jiaotong University, Xi’an, People’s Republic of China; 2Department of Otolaryngology-Head and Neck Surgery, The Sec...

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Autores principales:	Li XL, Ren XY, Liang JM, Ma WJ, Wang ZH, Yang ZQ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	liposome hemangioma VEGF2/KDR apoptosis Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/585a31b88c5a458d9a9617521fc3c7e2
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spelling	oai:doaj.org-article:585a31b88c5a458d9a9617521fc3c7e22021-12-02T01:04:58ZDelivery of sodium morrhuate to hemangioma endothelial cells using immunoliposomes conjugated with anti-VEGFR2/KDR antibody1178-2013https://doaj.org/article/585a31b88c5a458d9a9617521fc3c7e22017-09-01T00:00:00Zhttps://www.dovepress.com/delivery-of-sodium-morrhuate-to-hemangioma-endothelial-cells-using-imm-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Xiaoli Li,1 Xiaoyong Ren,2 Jianmin Liang,2 Weijun Ma,2 Zhenghui Wang,2 Zhuangqun Yang3 1Department of Dermatology, The Second Affiliated Hospital, Xi’an Jiaotong University, Xi’an, People’s Republic of China; 2Department of Otolaryngology-Head and Neck Surgery, The Second Affiliated Hospital, Xi’an Jiaotong University, Xi’an, People’s Republic of China; 3Department of Plastic and Burns Surgery, The First Affiliated Hospital, Xi’an Jiaotong University, Xi’an, People’s Republic of China Abstract: Hemangioma is a common benign tumor affecting infants. In this study, we prepared sodium morrhuate immunoliposomes through encapsulation of sodium morrhuate with liposomes coupled with an anti-VEGFR2/KDR antibody and examined its effect on the biology of human hemangioma endothelial cells (HECs). It was found that compared to the liposomal sodium morrhuate group, treatment with sodium morrhuate immunoliposomes facilitated cell detachment and apoptotic death. Confocal microscopy analysis revealed that sodium morrhuate immunoliposomes had a higher binding activity to HECs than liposomal sodium morrhuate. Apoptosis analysis further demonstrated that treatment with liposomal sodium morrhuate or sodium morrhuate immunoliposomes significantly induced apoptosis in HECs, compared to the control group. Western blot analysis revealed an induction of caspase-3 and caspase-9 levels and reduction of caspase-8 and Bcl-2 levels in HECs treated with liposomal sodium morrhuate or sodium morrhuate immunoliposomes. Taken together, these results indicate that sodium morrhuate immunoliposomes have an increased capacity to target HECs and promote mitochondrial apoptosis. Therefore, sodium morrhuate immunoliposomes may represent a promising agent in the treatment of hemangiomas. Keywords: liposome, hemangioma, VEGF2, KDR, apoptosisLi XLRen XYLiang JMMa WJWang ZHYang ZQDove Medical PressarticleliposomehemangiomaVEGF2/KDRapoptosisMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 6963-6972 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	liposome hemangioma VEGF2/KDR apoptosis Medicine (General) R5-920
spellingShingle	liposome hemangioma VEGF2/KDR apoptosis Medicine (General) R5-920 Li XL Ren XY Liang JM Ma WJ Wang ZH Yang ZQ Delivery of sodium morrhuate to hemangioma endothelial cells using immunoliposomes conjugated with anti-VEGFR2/KDR antibody
description	Xiaoli Li,1 Xiaoyong Ren,2 Jianmin Liang,2 Weijun Ma,2 Zhenghui Wang,2 Zhuangqun Yang3 1Department of Dermatology, The Second Affiliated Hospital, Xi’an Jiaotong University, Xi’an, People’s Republic of China; 2Department of Otolaryngology-Head and Neck Surgery, The Second Affiliated Hospital, Xi’an Jiaotong University, Xi’an, People’s Republic of China; 3Department of Plastic and Burns Surgery, The First Affiliated Hospital, Xi’an Jiaotong University, Xi’an, People’s Republic of China Abstract: Hemangioma is a common benign tumor affecting infants. In this study, we prepared sodium morrhuate immunoliposomes through encapsulation of sodium morrhuate with liposomes coupled with an anti-VEGFR2/KDR antibody and examined its effect on the biology of human hemangioma endothelial cells (HECs). It was found that compared to the liposomal sodium morrhuate group, treatment with sodium morrhuate immunoliposomes facilitated cell detachment and apoptotic death. Confocal microscopy analysis revealed that sodium morrhuate immunoliposomes had a higher binding activity to HECs than liposomal sodium morrhuate. Apoptosis analysis further demonstrated that treatment with liposomal sodium morrhuate or sodium morrhuate immunoliposomes significantly induced apoptosis in HECs, compared to the control group. Western blot analysis revealed an induction of caspase-3 and caspase-9 levels and reduction of caspase-8 and Bcl-2 levels in HECs treated with liposomal sodium morrhuate or sodium morrhuate immunoliposomes. Taken together, these results indicate that sodium morrhuate immunoliposomes have an increased capacity to target HECs and promote mitochondrial apoptosis. Therefore, sodium morrhuate immunoliposomes may represent a promising agent in the treatment of hemangiomas. Keywords: liposome, hemangioma, VEGF2, KDR, apoptosis
format	article
author	Li XL Ren XY Liang JM Ma WJ Wang ZH Yang ZQ
author_facet	Li XL Ren XY Liang JM Ma WJ Wang ZH Yang ZQ
author_sort	Li XL
title	Delivery of sodium morrhuate to hemangioma endothelial cells using immunoliposomes conjugated with anti-VEGFR2/KDR antibody
title_short	Delivery of sodium morrhuate to hemangioma endothelial cells using immunoliposomes conjugated with anti-VEGFR2/KDR antibody
title_full	Delivery of sodium morrhuate to hemangioma endothelial cells using immunoliposomes conjugated with anti-VEGFR2/KDR antibody
title_fullStr	Delivery of sodium morrhuate to hemangioma endothelial cells using immunoliposomes conjugated with anti-VEGFR2/KDR antibody
title_full_unstemmed	Delivery of sodium morrhuate to hemangioma endothelial cells using immunoliposomes conjugated with anti-VEGFR2/KDR antibody
title_sort	delivery of sodium morrhuate to hemangioma endothelial cells using immunoliposomes conjugated with anti-vegfr2/kdr antibody
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/585a31b88c5a458d9a9617521fc3c7e2
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_version_	1718403317464301568

Delivery of sodium morrhuate to hemangioma endothelial cells using immunoliposomes conjugated with anti-VEGFR2/KDR antibody

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