Green Synthesis and Characterization of Carboxymethyl Cellulose Fabricated Silver-Based Nanocomposite for Various Therapeutic Applications

Muhammad Arif Asghar,1 Rabia Ismail Yousuf,1 Muhammad Harris Shoaib,1 Muhammad Asif Asghar,2 Mehrukh Zehravi,3 Ahad Abdul Rehman,4 Muhammad Suleman Imtiaz,5 Kamran Khan5 1Department of Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences, University of Karachi, Karachi, Pakistan; 2Food and...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Asghar MA, Yousuf RI, Shoaib MH, Zehravi M, Rehman AA, Imtiaz MS, Khan K
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	silver nanoparticles syzygium aromaticum carboxymethyl cellulose green synthesis biological applications Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/586122cedff3471c8dfb7507de927deb
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:586122cedff3471c8dfb7507de927deb
record_format	dspace
institution	DOAJ
collection	DOAJ
language	EN
topic	silver nanoparticles syzygium aromaticum carboxymethyl cellulose green synthesis biological applications Medicine (General) R5-920
spellingShingle	silver nanoparticles syzygium aromaticum carboxymethyl cellulose green synthesis biological applications Medicine (General) R5-920 Asghar MA Yousuf RI Shoaib MH Asghar MA Zehravi M Rehman AA Imtiaz MS Khan K Green Synthesis and Characterization of Carboxymethyl Cellulose Fabricated Silver-Based Nanocomposite for Various Therapeutic Applications
description	Muhammad Arif Asghar,1 Rabia Ismail Yousuf,1 Muhammad Harris Shoaib,1 Muhammad Asif Asghar,2 Mehrukh Zehravi,3 Ahad Abdul Rehman,4 Muhammad Suleman Imtiaz,5 Kamran Khan5 1Department of Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences, University of Karachi, Karachi, Pakistan; 2Food and Feed Safety Laboratory, Food and Marine Resources Research Centre, PCSIR Laboratories Complex, Karachi, Sindh, Pakistan; 3Department of Clinical Pharmacy, College of Pharmacy for Girls, Prince Sattam Bin Abdul Aziz University, Al-Kharj, Kingdom of Saudi Arabia; 4Department of Pharmacology, Institute of Pharmaceutical Sciences, Jinnah Sindh Medical University, Karachi, Pakistan; 5Department of Pharmaceutics, Institute of Pharmaceutical Sciences, Jinnah Sindh Medical University, Karachi, PakistanCorrespondence: Rabia Ismail Yousuf; Muhammad Harris ShoaibDepartment of Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences, University of Karachi, Karachi, PakistanEmail rabia_pharmaceutics@yahoo.com; harrisshoaib2000@yahoo.com; mhshoaib@uok.edu.pkPurpose: The current study proposed the simple, eco-friendly and cost-effective synthesis of carboxymethyl cellulose (CMC) structured silver-based nanocomposite (CMC-AgNPs) using Syzygium aromaticum buds extract.Methods: The CMC-AgNPs were characterized by ultraviolet (UV) spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transmission infra-red (FTIR), energy-dispersive X-ray (EDX), and dynamic light scattering (DLS) techniques. The synthesized nanocomposites were evaluated for their bactericidal kinetics, in-vivo anti-inflammatory, anti-leishmaniasis, antioxidant and cytotoxic activities using different in-vitro and in-vivo models.Results: The spherical shape nanocomposite of CMC-AgNPs was synthesized with the mean size range of 20– 30 nm, and the average pore diameter is 18.2 nm while the mean zeta potential of − 31.6 ± 3.64 mV. The highly significant (P < 0.005) antibacterial activity was found against six bacterial strains with the ZIs of 24.6 to 27.9 mm. More drop counts were observed in Gram-negative strains after 10 min exposure with CMC-AgNPs. Significant damage in bacterial cell membrane was also observed in atomic force microscopy (AFM) after treated with CMC-AgNPs. Nanocomposite showed highly significant anti-inflammatory activity in cotton pellet induced granuloma model (Phase I) in rats with the mean inhibitions of 43.13% and 48.68% at the doses of 0.025 and 0.05 mg/kg, respectively, when compared to control. Reduction in rat paw edema (Phase II) was also highly significant (0.025 mg/kg; 42.39%; 0.05 mg/kg, 47.82%). At dose of 0.05 mg/kg, CMC-AgNPs caused highly significant decrease in leukocyte counts (922 ± 83), levels of CRP (8.4 ± 0.73 mg/mL), IL-1 (177.4 ± 21.3 pg/mL), IL-2 (83.7 ± 11.5 pg/mL), IL-6 (83.7 ± 11.5 pg/mL) and TNF-α (18.3 ± 5.3 pg/mL) as compared to control group. CMC-AgNPs produced highly effective anti-leishmaniasis activity with the viable Leishmania major counts decreased up to 36.7% within 24 h, and the IC50 was found to be 28.41 μg/mL. The potent DPPH radical scavenging potential was also observed for CMC-AgNPs with the IC50 value of 112 μg/mL. Furthermore, the cytotoxicity was assessed using HeLa cell lines with the LC50 of 108.2 μg/mL.Conclusion: The current findings demonstrate positive attributes of CMC fabricated AgNPs as a promising antibacterial, anti-inflammatory, anti-leishmaniasis, and antioxidant agent with low cytotoxic potential.Keywords: silver nanoparticles, Syzygium aromaticum, carboxymethyl cellulose, green synthesis, biological applications
format	article
author	Asghar MA Yousuf RI Shoaib MH Asghar MA Zehravi M Rehman AA Imtiaz MS Khan K
author_facet	Asghar MA Yousuf RI Shoaib MH Asghar MA Zehravi M Rehman AA Imtiaz MS Khan K
author_sort	Asghar MA
title	Green Synthesis and Characterization of Carboxymethyl Cellulose Fabricated Silver-Based Nanocomposite for Various Therapeutic Applications
title_short	Green Synthesis and Characterization of Carboxymethyl Cellulose Fabricated Silver-Based Nanocomposite for Various Therapeutic Applications
title_full	Green Synthesis and Characterization of Carboxymethyl Cellulose Fabricated Silver-Based Nanocomposite for Various Therapeutic Applications
title_fullStr	Green Synthesis and Characterization of Carboxymethyl Cellulose Fabricated Silver-Based Nanocomposite for Various Therapeutic Applications
title_full_unstemmed	Green Synthesis and Characterization of Carboxymethyl Cellulose Fabricated Silver-Based Nanocomposite for Various Therapeutic Applications
title_sort	green synthesis and characterization of carboxymethyl cellulose fabricated silver-based nanocomposite for various therapeutic applications
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/586122cedff3471c8dfb7507de927deb
work_keys_str_mv	AT asgharma greensynthesisandcharacterizationofcarboxymethylcellulosefabricatedsilverbasednanocompositeforvarioustherapeuticapplications AT yousufri greensynthesisandcharacterizationofcarboxymethylcellulosefabricatedsilverbasednanocompositeforvarioustherapeuticapplications AT shoaibmh greensynthesisandcharacterizationofcarboxymethylcellulosefabricatedsilverbasednanocompositeforvarioustherapeuticapplications AT asgharma greensynthesisandcharacterizationofcarboxymethylcellulosefabricatedsilverbasednanocompositeforvarioustherapeuticapplications AT zehravim greensynthesisandcharacterizationofcarboxymethylcellulosefabricatedsilverbasednanocompositeforvarioustherapeuticapplications AT rehmanaa greensynthesisandcharacterizationofcarboxymethylcellulosefabricatedsilverbasednanocompositeforvarioustherapeuticapplications AT imtiazms greensynthesisandcharacterizationofcarboxymethylcellulosefabricatedsilverbasednanocompositeforvarioustherapeuticapplications AT khank greensynthesisandcharacterizationofcarboxymethylcellulosefabricatedsilverbasednanocompositeforvarioustherapeuticapplications
_version_	1718377211342356480
spelling	oai:doaj.org-article:586122cedff3471c8dfb7507de927deb2021-12-02T19:03:47ZGreen Synthesis and Characterization of Carboxymethyl Cellulose Fabricated Silver-Based Nanocomposite for Various Therapeutic Applications1178-2013https://doaj.org/article/586122cedff3471c8dfb7507de927deb2021-08-01T00:00:00Zhttps://www.dovepress.com/green-synthesis-and-characterization-of-carboxymethyl-cellulose-fabric-peer-reviewed-fulltext-article-IJNhttps://doaj.org/toc/1178-2013Muhammad Arif Asghar,1 Rabia Ismail Yousuf,1 Muhammad Harris Shoaib,1 Muhammad Asif Asghar,2 Mehrukh Zehravi,3 Ahad Abdul Rehman,4 Muhammad Suleman Imtiaz,5 Kamran Khan5 1Department of Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences, University of Karachi, Karachi, Pakistan; 2Food and Feed Safety Laboratory, Food and Marine Resources Research Centre, PCSIR Laboratories Complex, Karachi, Sindh, Pakistan; 3Department of Clinical Pharmacy, College of Pharmacy for Girls, Prince Sattam Bin Abdul Aziz University, Al-Kharj, Kingdom of Saudi Arabia; 4Department of Pharmacology, Institute of Pharmaceutical Sciences, Jinnah Sindh Medical University, Karachi, Pakistan; 5Department of Pharmaceutics, Institute of Pharmaceutical Sciences, Jinnah Sindh Medical University, Karachi, PakistanCorrespondence: Rabia Ismail Yousuf; Muhammad Harris ShoaibDepartment of Pharmaceutics, Faculty of Pharmacy and Pharmaceutical Sciences, University of Karachi, Karachi, PakistanEmail rabia_pharmaceutics@yahoo.com; harrisshoaib2000@yahoo.com; mhshoaib@uok.edu.pkPurpose: The current study proposed the simple, eco-friendly and cost-effective synthesis of carboxymethyl cellulose (CMC) structured silver-based nanocomposite (CMC-AgNPs) using Syzygium aromaticum buds extract.Methods: The CMC-AgNPs were characterized by ultraviolet (UV) spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), Fourier transmission infra-red (FTIR), energy-dispersive X-ray (EDX), and dynamic light scattering (DLS) techniques. The synthesized nanocomposites were evaluated for their bactericidal kinetics, in-vivo anti-inflammatory, anti-leishmaniasis, antioxidant and cytotoxic activities using different in-vitro and in-vivo models.Results: The spherical shape nanocomposite of CMC-AgNPs was synthesized with the mean size range of 20– 30 nm, and the average pore diameter is 18.2 nm while the mean zeta potential of − 31.6 ± 3.64 mV. The highly significant (P < 0.005) antibacterial activity was found against six bacterial strains with the ZIs of 24.6 to 27.9 mm. More drop counts were observed in Gram-negative strains after 10 min exposure with CMC-AgNPs. Significant damage in bacterial cell membrane was also observed in atomic force microscopy (AFM) after treated with CMC-AgNPs. Nanocomposite showed highly significant anti-inflammatory activity in cotton pellet induced granuloma model (Phase I) in rats with the mean inhibitions of 43.13% and 48.68% at the doses of 0.025 and 0.05 mg/kg, respectively, when compared to control. Reduction in rat paw edema (Phase II) was also highly significant (0.025 mg/kg; 42.39%; 0.05 mg/kg, 47.82%). At dose of 0.05 mg/kg, CMC-AgNPs caused highly significant decrease in leukocyte counts (922 ± 83), levels of CRP (8.4 ± 0.73 mg/mL), IL-1 (177.4 ± 21.3 pg/mL), IL-2 (83.7 ± 11.5 pg/mL), IL-6 (83.7 ± 11.5 pg/mL) and TNF-α (18.3 ± 5.3 pg/mL) as compared to control group. CMC-AgNPs produced highly effective anti-leishmaniasis activity with the viable Leishmania major counts decreased up to 36.7% within 24 h, and the IC50 was found to be 28.41 μg/mL. The potent DPPH radical scavenging potential was also observed for CMC-AgNPs with the IC50 value of 112 μg/mL. Furthermore, the cytotoxicity was assessed using HeLa cell lines with the LC50 of 108.2 μg/mL.Conclusion: The current findings demonstrate positive attributes of CMC fabricated AgNPs as a promising antibacterial, anti-inflammatory, anti-leishmaniasis, and antioxidant agent with low cytotoxic potential.Keywords: silver nanoparticles, Syzygium aromaticum, carboxymethyl cellulose, green synthesis, biological applicationsAsghar MAYousuf RIShoaib MHAsghar MAZehravi MRehman AAImtiaz MSKhan KDove Medical Pressarticlesilver nanoparticlessyzygium aromaticumcarboxymethyl cellulosegreen synthesisbiological applicationsMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 5371-5393 (2021)

Green Synthesis and Characterization of Carboxymethyl Cellulose Fabricated Silver-Based Nanocomposite for Various Therapeutic Applications

Ejemplares similares