Mutational Effects of Mobile Introns on the Mitochondrial Genomes of Metschnikowia Yeasts

It has been argued that DNA repair by homologous recombination in the context of endonuclease-mediated cleavage can cause mutations. To better understand this phenomenon, we examined homologous recombination following endonuclease cleavage in a native genomic context: the movement of self-splicing i...

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Autores principales:	Scout R. L. Thompson, Dong Kyung Lee, Marc-André Lachance, David Roy Smith
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	endonuclease homologous recombination selfish elements yeast mobile intron Genetics QH426-470
Acceso en línea:	https://doaj.org/article/586135a027554a458b202f002ae3ee15
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spelling	oai:doaj.org-article:586135a027554a458b202f002ae3ee152021-11-04T08:53:30ZMutational Effects of Mobile Introns on the Mitochondrial Genomes of Metschnikowia Yeasts1664-802110.3389/fgene.2021.785218https://doaj.org/article/586135a027554a458b202f002ae3ee152021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fgene.2021.785218/fullhttps://doaj.org/toc/1664-8021It has been argued that DNA repair by homologous recombination in the context of endonuclease-mediated cleavage can cause mutations. To better understand this phenomenon, we examined homologous recombination following endonuclease cleavage in a native genomic context: the movement of self-splicing introns in the mitochondrial genomes of Metschnikowia yeasts. Self-splicing mitochondrial introns are mobile elements, which can copy and paste themselves at specific insertion sites in mitochondrial DNA using a homing endonuclease in conjunction with homologous recombination. Here, we explore the mutational effects of self-splicing introns by comparing sequence variation within the intron-rich cox1 and cob genes from 71 strains (belonging to 40 species) from the yeast genus Metschnikowia. We observed a higher density of single nucleotide polymorphisms around self-splicing-intron insertion sites. Given what is currently known about the movement of organelle introns, it is likely that their mutational effects result from the high binding affinity of endonucleases and their interference with repair machinery during homologous recombination (or, alternatively, via gene conversion occurring during the intron insertion process). These findings suggest that there are fitness costs to harbouring self-splicing, mobile introns and will help us better understand the risks associated with modern biotechnologies that use endonuclease-mediated homologous recombination, such as CRISPR-Cas9 gene editing.Scout R. L. ThompsonDong Kyung LeeMarc-André LachanceDavid Roy SmithFrontiers Media S.A.articleendonucleasehomologous recombinationselfish elementsyeastmobile intronGeneticsQH426-470ENFrontiers in Genetics, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	endonuclease homologous recombination selfish elements yeast mobile intron Genetics QH426-470
spellingShingle	endonuclease homologous recombination selfish elements yeast mobile intron Genetics QH426-470 Scout R. L. Thompson Dong Kyung Lee Marc-André Lachance David Roy Smith Mutational Effects of Mobile Introns on the Mitochondrial Genomes of Metschnikowia Yeasts
description	It has been argued that DNA repair by homologous recombination in the context of endonuclease-mediated cleavage can cause mutations. To better understand this phenomenon, we examined homologous recombination following endonuclease cleavage in a native genomic context: the movement of self-splicing introns in the mitochondrial genomes of Metschnikowia yeasts. Self-splicing mitochondrial introns are mobile elements, which can copy and paste themselves at specific insertion sites in mitochondrial DNA using a homing endonuclease in conjunction with homologous recombination. Here, we explore the mutational effects of self-splicing introns by comparing sequence variation within the intron-rich cox1 and cob genes from 71 strains (belonging to 40 species) from the yeast genus Metschnikowia. We observed a higher density of single nucleotide polymorphisms around self-splicing-intron insertion sites. Given what is currently known about the movement of organelle introns, it is likely that their mutational effects result from the high binding affinity of endonucleases and their interference with repair machinery during homologous recombination (or, alternatively, via gene conversion occurring during the intron insertion process). These findings suggest that there are fitness costs to harbouring self-splicing, mobile introns and will help us better understand the risks associated with modern biotechnologies that use endonuclease-mediated homologous recombination, such as CRISPR-Cas9 gene editing.
format	article
author	Scout R. L. Thompson Dong Kyung Lee Marc-André Lachance David Roy Smith
author_facet	Scout R. L. Thompson Dong Kyung Lee Marc-André Lachance David Roy Smith
author_sort	Scout R. L. Thompson
title	Mutational Effects of Mobile Introns on the Mitochondrial Genomes of Metschnikowia Yeasts
title_short	Mutational Effects of Mobile Introns on the Mitochondrial Genomes of Metschnikowia Yeasts
title_full	Mutational Effects of Mobile Introns on the Mitochondrial Genomes of Metschnikowia Yeasts
title_fullStr	Mutational Effects of Mobile Introns on the Mitochondrial Genomes of Metschnikowia Yeasts
title_full_unstemmed	Mutational Effects of Mobile Introns on the Mitochondrial Genomes of Metschnikowia Yeasts
title_sort	mutational effects of mobile introns on the mitochondrial genomes of metschnikowia yeasts
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/586135a027554a458b202f002ae3ee15
work_keys_str_mv	AT scoutrlthompson mutationaleffectsofmobileintronsonthemitochondrialgenomesofmetschnikowiayeasts AT dongkyunglee mutationaleffectsofmobileintronsonthemitochondrialgenomesofmetschnikowiayeasts AT marcandrelachance mutationaleffectsofmobileintronsonthemitochondrialgenomesofmetschnikowiayeasts AT davidroysmith mutationaleffectsofmobileintronsonthemitochondrialgenomesofmetschnikowiayeasts
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Mutational Effects of Mobile Introns on the Mitochondrial Genomes of Metschnikowia Yeasts

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