Global analysis of lysine 2-hydroxyisobutyrylation in wheat root

Abstract Lysine 2-hydroxyisobutyrylation (Khib) is a novel naturally occurring post-translational modification. The system Khib identification at proteomics level has been performed in various species and tissues to characterize the role of Khib in biological activities. However, the study of Khib i...

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Autores principales:	Feng Bo, Li Shengdong, Wang Zongshuai, Cao Fang, Wang Zheng, Gao Chunhua, Li Geng, Kong Ling’an
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5861b96d181d4267af487d8c73674e10
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spelling	oai:doaj.org-article:5861b96d181d4267af487d8c73674e102021-12-02T16:30:18ZGlobal analysis of lysine 2-hydroxyisobutyrylation in wheat root10.1038/s41598-021-85879-y2045-2322https://doaj.org/article/5861b96d181d4267af487d8c73674e102021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85879-yhttps://doaj.org/toc/2045-2322Abstract Lysine 2-hydroxyisobutyrylation (Khib) is a novel naturally occurring post-translational modification. The system Khib identification at proteomics level has been performed in various species and tissues to characterize the role of Khib in biological activities. However, the study of Khib in plant species is relatively less. In the present study, the first plant root tissues lysine 2-hydroxyisobutyrylome analysis was performed in wheat with antibody immunoprecipitation affinity, high resolution mass spectrometry-based proteomics and bioinformatics analysis. In total, 6328 Khib sites in 2186 proteins were repeatedly identified in three replicates. These Khib proteins showed a wide subcellular location distribution. Function and pathways characterization of these Khib proteins indicated that many cellular functions and metabolism pathways were potentially affected by this modification. Protein and amino acid metabolism related process may be regulated by Khib, especially ribosome activities and proteins biosynthesis process. Carbohydrate metabolism and energy production related processes including glycolysis/gluconeogenesis, TCA cycle and oxidative phosphorylation pathways were also affected by Khib modification. Besides, root sulfur assimilation and transformation related enzymes exhibited Khib modification. Our work illustrated the potential regulation role of Khib in wheat root physiology and biology, which could be used as a useful reference for Khib study in plant root.Feng BoLi ShengdongWang ZongshuaiCao FangWang ZhengGao ChunhuaLi GengKong Ling’anNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Feng Bo Li Shengdong Wang Zongshuai Cao Fang Wang Zheng Gao Chunhua Li Geng Kong Ling’an Global analysis of lysine 2-hydroxyisobutyrylation in wheat root
description	Abstract Lysine 2-hydroxyisobutyrylation (Khib) is a novel naturally occurring post-translational modification. The system Khib identification at proteomics level has been performed in various species and tissues to characterize the role of Khib in biological activities. However, the study of Khib in plant species is relatively less. In the present study, the first plant root tissues lysine 2-hydroxyisobutyrylome analysis was performed in wheat with antibody immunoprecipitation affinity, high resolution mass spectrometry-based proteomics and bioinformatics analysis. In total, 6328 Khib sites in 2186 proteins were repeatedly identified in three replicates. These Khib proteins showed a wide subcellular location distribution. Function and pathways characterization of these Khib proteins indicated that many cellular functions and metabolism pathways were potentially affected by this modification. Protein and amino acid metabolism related process may be regulated by Khib, especially ribosome activities and proteins biosynthesis process. Carbohydrate metabolism and energy production related processes including glycolysis/gluconeogenesis, TCA cycle and oxidative phosphorylation pathways were also affected by Khib modification. Besides, root sulfur assimilation and transformation related enzymes exhibited Khib modification. Our work illustrated the potential regulation role of Khib in wheat root physiology and biology, which could be used as a useful reference for Khib study in plant root.
format	article
author	Feng Bo Li Shengdong Wang Zongshuai Cao Fang Wang Zheng Gao Chunhua Li Geng Kong Ling’an
author_facet	Feng Bo Li Shengdong Wang Zongshuai Cao Fang Wang Zheng Gao Chunhua Li Geng Kong Ling’an
author_sort	Feng Bo
title	Global analysis of lysine 2-hydroxyisobutyrylation in wheat root
title_short	Global analysis of lysine 2-hydroxyisobutyrylation in wheat root
title_full	Global analysis of lysine 2-hydroxyisobutyrylation in wheat root
title_fullStr	Global analysis of lysine 2-hydroxyisobutyrylation in wheat root
title_full_unstemmed	Global analysis of lysine 2-hydroxyisobutyrylation in wheat root
title_sort	global analysis of lysine 2-hydroxyisobutyrylation in wheat root
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/5861b96d181d4267af487d8c73674e10
work_keys_str_mv	AT fengbo globalanalysisoflysine2hydroxyisobutyrylationinwheatroot AT lishengdong globalanalysisoflysine2hydroxyisobutyrylationinwheatroot AT wangzongshuai globalanalysisoflysine2hydroxyisobutyrylationinwheatroot AT caofang globalanalysisoflysine2hydroxyisobutyrylationinwheatroot AT wangzheng globalanalysisoflysine2hydroxyisobutyrylationinwheatroot AT gaochunhua globalanalysisoflysine2hydroxyisobutyrylationinwheatroot AT ligeng globalanalysisoflysine2hydroxyisobutyrylationinwheatroot AT konglingan globalanalysisoflysine2hydroxyisobutyrylationinwheatroot
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Global analysis of lysine 2-hydroxyisobutyrylation in wheat root

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