High Genomic Identity between Clinical and Environmental Strains of Herbaspirillum frisingense Suggests Pre-Adaptation to Different Hosts and Intrinsic Resistance to Multiple Drugs

The genus Herbaspirillum is widely studied for its ability to associate with grasses and to perform biological nitrogen fixation. However, the bacteria of the Herbaspirillum genus have frequently been isolated from clinical samples. Understanding the genomic cha...

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Autores principales:	Willian Klassen Oliveira, Hugo Leonardo Ávila, Michelle Zibeti Tadra, Rodrigo Luiz Cardoso, Cyntia Maria Teles Fadel-Pichet, Emanuel Maltempi de Souza, Fábio de Oliveira Pedrosa, Helisson Faoro
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>H. frisingense</i> AMR host–pathogen interaction genome comparison Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/586435a6f39c41dbbd8115c0e3b97261
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spelling	oai:doaj.org-article:586435a6f39c41dbbd8115c0e3b972612021-11-25T16:24:55ZHigh Genomic Identity between Clinical and Environmental Strains of <i>Herbaspirillum frisingense</i> Suggests Pre-Adaptation to Different Hosts and Intrinsic Resistance to Multiple Drugs10.3390/antibiotics101114092079-6382https://doaj.org/article/586435a6f39c41dbbd8115c0e3b972612021-11-01T00:00:00Zhttps://www.mdpi.com/2079-6382/10/11/1409https://doaj.org/toc/2079-6382The genus <i>Herbaspirillum</i> is widely studied for its ability to associate with grasses and to perform biological nitrogen fixation. However, the bacteria of the <i>Herbaspirillum</i> genus have frequently been isolated from clinical samples. Understanding the genomic characteristics that allow these bacteria to switch environments and become able to colonize human hosts is essential for monitoring emerging pathogens and predicting outbreaks. In this work, we describe the sequencing, assembly, and annotation of the genome of <i>H. frisingense</i> AU14559 isolated from the sputum of patients with cystic fibrosis, and its comparison with the genomes of the uropathogenic strain VT-16–41 and the environmental strains GSF30, BH-1, IAC152, and SG826. The genes responsible for biological nitrogen fixation were absent from all strains except for GSF30. On the other hand, genes encoding virulence and host interaction factors were mostly shared with environmental strains. We also identified a large set of intrinsic antibiotic resistance genes that were shared across all strains. Unlike other strains, in addition to unique genomic islands, AU14559 has a mutation that renders the biosynthesis of rhamnose and its incorporation into the exopolysaccharide unfeasible. These data suggest that <i>H. frisingense</i> has characteristics that provide it with the metabolic diversity needed to infect and colonize human hosts.Willian Klassen OliveiraHugo Leonardo ÁvilaMichelle Zibeti TadraRodrigo Luiz CardosoCyntia Maria Teles Fadel-PichetEmanuel Maltempi de SouzaFábio de Oliveira PedrosaHelisson FaoroMDPI AGarticle<i>H. frisingense</i>AMRhost–pathogen interactiongenome comparisonTherapeutics. PharmacologyRM1-950ENAntibiotics, Vol 10, Iss 1409, p 1409 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>H. frisingense</i> AMR host–pathogen interaction genome comparison Therapeutics. Pharmacology RM1-950
spellingShingle	<i>H. frisingense</i> AMR host–pathogen interaction genome comparison Therapeutics. Pharmacology RM1-950 Willian Klassen Oliveira Hugo Leonardo Ávila Michelle Zibeti Tadra Rodrigo Luiz Cardoso Cyntia Maria Teles Fadel-Pichet Emanuel Maltempi de Souza Fábio de Oliveira Pedrosa Helisson Faoro High Genomic Identity between Clinical and Environmental Strains of <i>Herbaspirillum frisingense</i> Suggests Pre-Adaptation to Different Hosts and Intrinsic Resistance to Multiple Drugs
description	The genus <i>Herbaspirillum</i> is widely studied for its ability to associate with grasses and to perform biological nitrogen fixation. However, the bacteria of the <i>Herbaspirillum</i> genus have frequently been isolated from clinical samples. Understanding the genomic characteristics that allow these bacteria to switch environments and become able to colonize human hosts is essential for monitoring emerging pathogens and predicting outbreaks. In this work, we describe the sequencing, assembly, and annotation of the genome of <i>H. frisingense</i> AU14559 isolated from the sputum of patients with cystic fibrosis, and its comparison with the genomes of the uropathogenic strain VT-16–41 and the environmental strains GSF30, BH-1, IAC152, and SG826. The genes responsible for biological nitrogen fixation were absent from all strains except for GSF30. On the other hand, genes encoding virulence and host interaction factors were mostly shared with environmental strains. We also identified a large set of intrinsic antibiotic resistance genes that were shared across all strains. Unlike other strains, in addition to unique genomic islands, AU14559 has a mutation that renders the biosynthesis of rhamnose and its incorporation into the exopolysaccharide unfeasible. These data suggest that <i>H. frisingense</i> has characteristics that provide it with the metabolic diversity needed to infect and colonize human hosts.
format	article
author	Willian Klassen Oliveira Hugo Leonardo Ávila Michelle Zibeti Tadra Rodrigo Luiz Cardoso Cyntia Maria Teles Fadel-Pichet Emanuel Maltempi de Souza Fábio de Oliveira Pedrosa Helisson Faoro
author_facet	Willian Klassen Oliveira Hugo Leonardo Ávila Michelle Zibeti Tadra Rodrigo Luiz Cardoso Cyntia Maria Teles Fadel-Pichet Emanuel Maltempi de Souza Fábio de Oliveira Pedrosa Helisson Faoro
author_sort	Willian Klassen Oliveira
title	High Genomic Identity between Clinical and Environmental Strains of <i>Herbaspirillum frisingense</i> Suggests Pre-Adaptation to Different Hosts and Intrinsic Resistance to Multiple Drugs
title_short	High Genomic Identity between Clinical and Environmental Strains of <i>Herbaspirillum frisingense</i> Suggests Pre-Adaptation to Different Hosts and Intrinsic Resistance to Multiple Drugs
title_full	High Genomic Identity between Clinical and Environmental Strains of <i>Herbaspirillum frisingense</i> Suggests Pre-Adaptation to Different Hosts and Intrinsic Resistance to Multiple Drugs
title_fullStr	High Genomic Identity between Clinical and Environmental Strains of <i>Herbaspirillum frisingense</i> Suggests Pre-Adaptation to Different Hosts and Intrinsic Resistance to Multiple Drugs
title_full_unstemmed	High Genomic Identity between Clinical and Environmental Strains of <i>Herbaspirillum frisingense</i> Suggests Pre-Adaptation to Different Hosts and Intrinsic Resistance to Multiple Drugs
title_sort	high genomic identity between clinical and environmental strains of <i>herbaspirillum frisingense</i> suggests pre-adaptation to different hosts and intrinsic resistance to multiple drugs
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/586435a6f39c41dbbd8115c0e3b97261
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High Genomic Identity between Clinical and Environmental Strains of <i>Herbaspirillum frisingense</i> Suggests Pre-Adaptation to Different Hosts and Intrinsic Resistance to Multiple Drugs

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