Identification of single-copy orthologous genes between Physalis and Solanum lycopersicum and analysis of genetic diversity in Physalis using molecular markers.

The genus Physalis includes a number of commercially important edible and ornamental species. Its high nutritional value and potential medicinal properties leads to the increased commercial interest in the products of this genus worldwide. However, lack of molecular markers prevents the detailed stu...

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Autores principales:	Jingli Wei, Xiaorong Hu, Jingjing Yang, Wencai Yang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/586538fe005848ab99316bc66d792054
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spelling	oai:doaj.org-article:586538fe005848ab99316bc66d7920542021-11-18T08:08:24ZIdentification of single-copy orthologous genes between Physalis and Solanum lycopersicum and analysis of genetic diversity in Physalis using molecular markers.1932-620310.1371/journal.pone.0050164https://doaj.org/article/586538fe005848ab99316bc66d7920542012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23166835/?tool=EBIhttps://doaj.org/toc/1932-6203The genus Physalis includes a number of commercially important edible and ornamental species. Its high nutritional value and potential medicinal properties leads to the increased commercial interest in the products of this genus worldwide. However, lack of molecular markers prevents the detailed study of genetics and phylogeny in Physalis, which limits the progress of breeding. In the present study, we compared the DNA sequences between Physalis and tomato, and attempted to analyze genetic diversity in Physalis using tomato markers. Blasting 23180 DNA sequences derived from Physalis against the International Tomato Annotation Group (ITAG) Release2.3 Predicted CDS (SL2.40) discovered 3356 single-copy orthologous genes between them. A total of 38 accessions from at least six species of Physalis were subjected to genetic diversity analysis using 97 tomato markers and 25 SSR markers derived from P. peruviana. Majority (73.2%) of tomato markers could amplify DNA fragments from at least one accession of Physalis. Diversity in Physalis at molecular level was also detected. The average Nei's genetic distance between accessions was 0.3806 with a range of 0.2865 to 0.7091. These results indicated Physalis and tomato had similarity at both molecular marker and DNA sequence levels. Therefore, the molecular markers developed in tomato can be used in genetic study in Physalis.Jingli WeiXiaorong HuJingjing YangWencai YangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 11, p e50164 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jingli Wei Xiaorong Hu Jingjing Yang Wencai Yang Identification of single-copy orthologous genes between Physalis and Solanum lycopersicum and analysis of genetic diversity in Physalis using molecular markers.
description	The genus Physalis includes a number of commercially important edible and ornamental species. Its high nutritional value and potential medicinal properties leads to the increased commercial interest in the products of this genus worldwide. However, lack of molecular markers prevents the detailed study of genetics and phylogeny in Physalis, which limits the progress of breeding. In the present study, we compared the DNA sequences between Physalis and tomato, and attempted to analyze genetic diversity in Physalis using tomato markers. Blasting 23180 DNA sequences derived from Physalis against the International Tomato Annotation Group (ITAG) Release2.3 Predicted CDS (SL2.40) discovered 3356 single-copy orthologous genes between them. A total of 38 accessions from at least six species of Physalis were subjected to genetic diversity analysis using 97 tomato markers and 25 SSR markers derived from P. peruviana. Majority (73.2%) of tomato markers could amplify DNA fragments from at least one accession of Physalis. Diversity in Physalis at molecular level was also detected. The average Nei's genetic distance between accessions was 0.3806 with a range of 0.2865 to 0.7091. These results indicated Physalis and tomato had similarity at both molecular marker and DNA sequence levels. Therefore, the molecular markers developed in tomato can be used in genetic study in Physalis.
format	article
author	Jingli Wei Xiaorong Hu Jingjing Yang Wencai Yang
author_facet	Jingli Wei Xiaorong Hu Jingjing Yang Wencai Yang
author_sort	Jingli Wei
title	Identification of single-copy orthologous genes between Physalis and Solanum lycopersicum and analysis of genetic diversity in Physalis using molecular markers.
title_short	Identification of single-copy orthologous genes between Physalis and Solanum lycopersicum and analysis of genetic diversity in Physalis using molecular markers.
title_full	Identification of single-copy orthologous genes between Physalis and Solanum lycopersicum and analysis of genetic diversity in Physalis using molecular markers.
title_fullStr	Identification of single-copy orthologous genes between Physalis and Solanum lycopersicum and analysis of genetic diversity in Physalis using molecular markers.
title_full_unstemmed	Identification of single-copy orthologous genes between Physalis and Solanum lycopersicum and analysis of genetic diversity in Physalis using molecular markers.
title_sort	identification of single-copy orthologous genes between physalis and solanum lycopersicum and analysis of genetic diversity in physalis using molecular markers.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/586538fe005848ab99316bc66d792054
work_keys_str_mv	AT jingliwei identificationofsinglecopyorthologousgenesbetweenphysalisandsolanumlycopersicumandanalysisofgeneticdiversityinphysalisusingmolecularmarkers AT xiaoronghu identificationofsinglecopyorthologousgenesbetweenphysalisandsolanumlycopersicumandanalysisofgeneticdiversityinphysalisusingmolecularmarkers AT jingjingyang identificationofsinglecopyorthologousgenesbetweenphysalisandsolanumlycopersicumandanalysisofgeneticdiversityinphysalisusingmolecularmarkers AT wencaiyang identificationofsinglecopyorthologousgenesbetweenphysalisandsolanumlycopersicumandanalysisofgeneticdiversityinphysalisusingmolecularmarkers
_version_	1718422167925817344

Identification of single-copy orthologous genes between Physalis and Solanum lycopersicum and analysis of genetic diversity in Physalis using molecular markers.

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