An Assay System for Point-of-Care Diagnosis of Tuberculosis using Commercially Manufactured PCB Technology
Abstract Rapid advances in clinical technologies, detection sensitivity and analytical throughput have delivered a significant expansion in our knowledge of prognostic and diagnostic biomarkers in many common infectious diseases, such as Tuberculosis (TB). During the last decade, a significant numbe...
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Nature Portfolio
2017
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oai:doaj.org-article:586778300a44468e8f5cef4ac9b309362021-12-02T11:52:27ZAn Assay System for Point-of-Care Diagnosis of Tuberculosis using Commercially Manufactured PCB Technology10.1038/s41598-017-00783-82045-2322https://doaj.org/article/586778300a44468e8f5cef4ac9b309362017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00783-8https://doaj.org/toc/2045-2322Abstract Rapid advances in clinical technologies, detection sensitivity and analytical throughput have delivered a significant expansion in our knowledge of prognostic and diagnostic biomarkers in many common infectious diseases, such as Tuberculosis (TB). During the last decade, a significant number of approaches to TB diagnosis have been attempted at Point-of-Care (PoC), exploiting a large variation of techniques and materials. In this work, we describe an electronics-based Enzyme-Linked ImmunoSorbent Assay (eELISA), using a Lab-on-a-Printed Circuit Board (LoPCB) approach, for TB diagnosis based on cytokine detection. The test relies upon an electrochemical (amperometric) assay, comprising a high-precision bioinstrumentation board and amperometric sensors, produced exclusively using standard PCB manufacturing processes. Electrochemical detection uses standard Au and Ag electrodes together with a bespoke, low-power, multichannel, portable data-acquisition system. We demonstrate high-performance assay chemistry performed at microfluidic volumes on Au pads directly at the PCB surface with improved limit of detection (~10 pg/mL) over standard colorimetric ELISA methods. The assay has also been implemented in plasma, showing the utility of the system for medical applications. This work is a significant step towards the development of a low-cost, portable, high-precision diagnostic and monitoring technology, which once combined with appropriate PCB-based microfluidic networks will provide complete LoPCB platforms.Daniel EvansKonstantinos I. PapadimitriouLouise GreatheadNikolaos VasilakisPanagiotis PantelidisPeter KelleherHywel MorganThemistoklis ProdromakisNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017) |
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Medicine R Science Q |
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Medicine R Science Q Daniel Evans Konstantinos I. Papadimitriou Louise Greathead Nikolaos Vasilakis Panagiotis Pantelidis Peter Kelleher Hywel Morgan Themistoklis Prodromakis An Assay System for Point-of-Care Diagnosis of Tuberculosis using Commercially Manufactured PCB Technology |
| description |
Abstract Rapid advances in clinical technologies, detection sensitivity and analytical throughput have delivered a significant expansion in our knowledge of prognostic and diagnostic biomarkers in many common infectious diseases, such as Tuberculosis (TB). During the last decade, a significant number of approaches to TB diagnosis have been attempted at Point-of-Care (PoC), exploiting a large variation of techniques and materials. In this work, we describe an electronics-based Enzyme-Linked ImmunoSorbent Assay (eELISA), using a Lab-on-a-Printed Circuit Board (LoPCB) approach, for TB diagnosis based on cytokine detection. The test relies upon an electrochemical (amperometric) assay, comprising a high-precision bioinstrumentation board and amperometric sensors, produced exclusively using standard PCB manufacturing processes. Electrochemical detection uses standard Au and Ag electrodes together with a bespoke, low-power, multichannel, portable data-acquisition system. We demonstrate high-performance assay chemistry performed at microfluidic volumes on Au pads directly at the PCB surface with improved limit of detection (~10 pg/mL) over standard colorimetric ELISA methods. The assay has also been implemented in plasma, showing the utility of the system for medical applications. This work is a significant step towards the development of a low-cost, portable, high-precision diagnostic and monitoring technology, which once combined with appropriate PCB-based microfluidic networks will provide complete LoPCB platforms. |
| format |
article |
| author |
Daniel Evans Konstantinos I. Papadimitriou Louise Greathead Nikolaos Vasilakis Panagiotis Pantelidis Peter Kelleher Hywel Morgan Themistoklis Prodromakis |
| author_facet |
Daniel Evans Konstantinos I. Papadimitriou Louise Greathead Nikolaos Vasilakis Panagiotis Pantelidis Peter Kelleher Hywel Morgan Themistoklis Prodromakis |
| author_sort |
Daniel Evans |
| title |
An Assay System for Point-of-Care Diagnosis of Tuberculosis using Commercially Manufactured PCB Technology |
| title_short |
An Assay System for Point-of-Care Diagnosis of Tuberculosis using Commercially Manufactured PCB Technology |
| title_full |
An Assay System for Point-of-Care Diagnosis of Tuberculosis using Commercially Manufactured PCB Technology |
| title_fullStr |
An Assay System for Point-of-Care Diagnosis of Tuberculosis using Commercially Manufactured PCB Technology |
| title_full_unstemmed |
An Assay System for Point-of-Care Diagnosis of Tuberculosis using Commercially Manufactured PCB Technology |
| title_sort |
assay system for point-of-care diagnosis of tuberculosis using commercially manufactured pcb technology |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/586778300a44468e8f5cef4ac9b30936 |
| work_keys_str_mv |
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