Chronic heat stress delays immune system development and alters serotonin signaling in pre-weaned dairy calves.

Exposure to heat stress can alter the development and immune system function in dairy calves. Serotonin is an immunomodulatory biogenic amine that functions as a neurotransmitter and as a stress-response mediator. Our objectives were to characterize the patterns of serum serotonin concentrations and...

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Autores principales: Marcela G Marrero, Bethany Dado-Senn, Sena L Field, Guan Yang, John P Driver, Jimena Laporta
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Publicado: Public Library of Science (PLoS) 2021
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Acceso en línea:https://doaj.org/article/587a6b65b1b14ea9ae508148d32baaa5
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spelling oai:doaj.org-article:587a6b65b1b14ea9ae508148d32baaa52021-12-02T20:07:18ZChronic heat stress delays immune system development and alters serotonin signaling in pre-weaned dairy calves.1932-620310.1371/journal.pone.0252474https://doaj.org/article/587a6b65b1b14ea9ae508148d32baaa52021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0252474https://doaj.org/toc/1932-6203Exposure to heat stress can alter the development and immune system function in dairy calves. Serotonin is an immunomodulatory biogenic amine that functions as a neurotransmitter and as a stress-response mediator. Our objectives were to characterize the patterns of serum serotonin concentrations and the pattern of serotonin-related genes expressed by immune cells of calves exposed to chronic heat stress or heat stress abatement during early life, and to explore whether these might relate to immune system development. Dairy calves were exposed to chronic heat stress (HS; n = 6) or heat stress abatement (cooling, CL; n = 6) across the prenatal (late gestation, last 46 d) and postnatal (from birth to weaning, 56 d) developmental windows. Blood samples were collected to harvest serum (weekly, from d 1 to 49), to isolate of circulating leukocyte mRNA (at 1, 21 and 42 d of age) and characterize immune cell populations by flow cytometry (at 21 and 47 d of age). Calves exposed to chronic heat stress pre- and postnatally had lower red blood cell counts and lower circulating serotonin, immunoglobulin G, and B-lymphocytes compared to CL calves. Circulating blood leukocyte mRNA expression of serotonin receptors -1A, -1F, -4 and -5 was greater, while heat shock protein 70 and immune-related genes (i.e., TBX21, TLR4, and TGFβ) were lower in HS relative to CL calves. Peripheral blood leukocytes from all calves secreted serotonin and interleukin-6 after in-vitro lipopolysaccharide stimulation. However, the HS calves produced more serotonin and less interleukin-6 than CL calves when activated in-vitro. Together, our data suggest that providing heat stress abatement to dairy calves across prenatal and postnatal developmental windows might modulate the serotonin synthesis pathway in ways that may benefit humoral immunity against microbial pathogens.Marcela G MarreroBethany Dado-SennSena L FieldGuan YangJohn P DriverJimena LaportaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 6, p e0252474 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Marcela G Marrero
Bethany Dado-Senn
Sena L Field
Guan Yang
John P Driver
Jimena Laporta
Chronic heat stress delays immune system development and alters serotonin signaling in pre-weaned dairy calves.
description Exposure to heat stress can alter the development and immune system function in dairy calves. Serotonin is an immunomodulatory biogenic amine that functions as a neurotransmitter and as a stress-response mediator. Our objectives were to characterize the patterns of serum serotonin concentrations and the pattern of serotonin-related genes expressed by immune cells of calves exposed to chronic heat stress or heat stress abatement during early life, and to explore whether these might relate to immune system development. Dairy calves were exposed to chronic heat stress (HS; n = 6) or heat stress abatement (cooling, CL; n = 6) across the prenatal (late gestation, last 46 d) and postnatal (from birth to weaning, 56 d) developmental windows. Blood samples were collected to harvest serum (weekly, from d 1 to 49), to isolate of circulating leukocyte mRNA (at 1, 21 and 42 d of age) and characterize immune cell populations by flow cytometry (at 21 and 47 d of age). Calves exposed to chronic heat stress pre- and postnatally had lower red blood cell counts and lower circulating serotonin, immunoglobulin G, and B-lymphocytes compared to CL calves. Circulating blood leukocyte mRNA expression of serotonin receptors -1A, -1F, -4 and -5 was greater, while heat shock protein 70 and immune-related genes (i.e., TBX21, TLR4, and TGFβ) were lower in HS relative to CL calves. Peripheral blood leukocytes from all calves secreted serotonin and interleukin-6 after in-vitro lipopolysaccharide stimulation. However, the HS calves produced more serotonin and less interleukin-6 than CL calves when activated in-vitro. Together, our data suggest that providing heat stress abatement to dairy calves across prenatal and postnatal developmental windows might modulate the serotonin synthesis pathway in ways that may benefit humoral immunity against microbial pathogens.
format article
author Marcela G Marrero
Bethany Dado-Senn
Sena L Field
Guan Yang
John P Driver
Jimena Laporta
author_facet Marcela G Marrero
Bethany Dado-Senn
Sena L Field
Guan Yang
John P Driver
Jimena Laporta
author_sort Marcela G Marrero
title Chronic heat stress delays immune system development and alters serotonin signaling in pre-weaned dairy calves.
title_short Chronic heat stress delays immune system development and alters serotonin signaling in pre-weaned dairy calves.
title_full Chronic heat stress delays immune system development and alters serotonin signaling in pre-weaned dairy calves.
title_fullStr Chronic heat stress delays immune system development and alters serotonin signaling in pre-weaned dairy calves.
title_full_unstemmed Chronic heat stress delays immune system development and alters serotonin signaling in pre-weaned dairy calves.
title_sort chronic heat stress delays immune system development and alters serotonin signaling in pre-weaned dairy calves.
publisher Public Library of Science (PLoS)
publishDate 2021
url https://doaj.org/article/587a6b65b1b14ea9ae508148d32baaa5
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AT senalfield chronicheatstressdelaysimmunesystemdevelopmentandaltersserotoninsignalinginpreweaneddairycalves
AT guanyang chronicheatstressdelaysimmunesystemdevelopmentandaltersserotoninsignalinginpreweaneddairycalves
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