Chromomycin A3 banding and chromosomal mapping of 45S and 5S ribosomal RNA genes in bottle gourd

Ribosomal DNAs and various banding patterns are landmarks in molecular cytogenetics providing useful information for karyotyping and addressing individual chromosomes. Bottle gourd is the only cultivated species of the Lagenaria genus with high genetic diversity. After CMA3/DAPI fluorochrome banding...

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Autores principales:	Ahmet L. Tek, Hümeyra Yıldız, Kamran Khan, Bilge Ş. Yıldırım
Formato:	article
Lenguaje:	EN
Publicado:	Firenze University Press 2021
Materias:	bottle gourd chromomycin A3 fluorescence in situ hybridization local accessions ribosomal DNA 45S Biology (General) QH301-705.5 Cytology QH573-671
Acceso en línea:	https://doaj.org/article/58830e5352ad4b159488287ca6d45c8f
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spelling	oai:doaj.org-article:58830e5352ad4b159488287ca6d45c8f2021-11-28T20:31:44ZChromomycin A3 banding and chromosomal mapping of 45S and 5S ribosomal RNA genes in bottle gourd0008-71142165-5391https://doaj.org/article/58830e5352ad4b159488287ca6d45c8f2021-11-01T00:00:00Zhttps://riviste.fupress.net/index.php/caryologia/article/view/1134https://doaj.org/toc/0008-7114https://doaj.org/toc/2165-5391Ribosomal DNAs and various banding patterns are landmarks in molecular cytogenetics providing useful information for karyotyping and addressing individual chromosomes. Bottle gourd is the only cultivated species of the Lagenaria genus with high genetic diversity. After CMA3/DAPI fluorochrome banding we investigated the GC- and AT-rich regions in interphase nuclei of five different local accessions. Fluorescence in situ hybridization (FISH) was conducted to determine the number and location of 45S and 5S rDNAs in bottle gourd. Our results showed four strong CMA3 regions in interphase and on mitotic metaphase chromosomes. FISH revealed four strong signals of 45S rDNA at the termini of two metaphase chromosome pairs and terminal 5S rDNA signals at another pair of chromosomes. The presence of four positive CMA3 bands colocalizes with four 45S rDNA signals in all bottle gourd accessions. Our results allow distinguishing two out of eleven chromosome pairs of bottle gourd. Ahmet L. TekHümeyra YıldızKamran KhanBilge Ş. YıldırımFirenze University Pressarticlebottle gourdchromomycin A3fluorescence in situ hybridizationlocal accessionsribosomal DNA45SBiology (General)QH301-705.5CytologyQH573-671ENCaryologia (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bottle gourd chromomycin A3 fluorescence in situ hybridization local accessions ribosomal DNA 45S Biology (General) QH301-705.5 Cytology QH573-671
spellingShingle	bottle gourd chromomycin A3 fluorescence in situ hybridization local accessions ribosomal DNA 45S Biology (General) QH301-705.5 Cytology QH573-671 Ahmet L. Tek Hümeyra Yıldız Kamran Khan Bilge Ş. Yıldırım Chromomycin A3 banding and chromosomal mapping of 45S and 5S ribosomal RNA genes in bottle gourd
description	Ribosomal DNAs and various banding patterns are landmarks in molecular cytogenetics providing useful information for karyotyping and addressing individual chromosomes. Bottle gourd is the only cultivated species of the Lagenaria genus with high genetic diversity. After CMA3/DAPI fluorochrome banding we investigated the GC- and AT-rich regions in interphase nuclei of five different local accessions. Fluorescence in situ hybridization (FISH) was conducted to determine the number and location of 45S and 5S rDNAs in bottle gourd. Our results showed four strong CMA3 regions in interphase and on mitotic metaphase chromosomes. FISH revealed four strong signals of 45S rDNA at the termini of two metaphase chromosome pairs and terminal 5S rDNA signals at another pair of chromosomes. The presence of four positive CMA3 bands colocalizes with four 45S rDNA signals in all bottle gourd accessions. Our results allow distinguishing two out of eleven chromosome pairs of bottle gourd.
format	article
author	Ahmet L. Tek Hümeyra Yıldız Kamran Khan Bilge Ş. Yıldırım
author_facet	Ahmet L. Tek Hümeyra Yıldız Kamran Khan Bilge Ş. Yıldırım
author_sort	Ahmet L. Tek
title	Chromomycin A3 banding and chromosomal mapping of 45S and 5S ribosomal RNA genes in bottle gourd
title_short	Chromomycin A3 banding and chromosomal mapping of 45S and 5S ribosomal RNA genes in bottle gourd
title_full	Chromomycin A3 banding and chromosomal mapping of 45S and 5S ribosomal RNA genes in bottle gourd
title_fullStr	Chromomycin A3 banding and chromosomal mapping of 45S and 5S ribosomal RNA genes in bottle gourd
title_full_unstemmed	Chromomycin A3 banding and chromosomal mapping of 45S and 5S ribosomal RNA genes in bottle gourd
title_sort	chromomycin a3 banding and chromosomal mapping of 45s and 5s ribosomal rna genes in bottle gourd
publisher	Firenze University Press
publishDate	2021
url	https://doaj.org/article/58830e5352ad4b159488287ca6d45c8f
work_keys_str_mv	AT ahmetltek chromomycina3bandingandchromosomalmappingof45sand5sribosomalrnagenesinbottlegourd AT humeyrayıldız chromomycina3bandingandchromosomalmappingof45sand5sribosomalrnagenesinbottlegourd AT kamrankhan chromomycina3bandingandchromosomalmappingof45sand5sribosomalrnagenesinbottlegourd AT bilgesyıldırım chromomycina3bandingandchromosomalmappingof45sand5sribosomalrnagenesinbottlegourd
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Chromomycin A3 banding and chromosomal mapping of 45S and 5S ribosomal RNA genes in bottle gourd

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