Identification and expression analysis of the glycosyltransferase GT43 family members in bamboo reveal their potential function in xylan biosynthesis during rapid growth

Abstract Background Xylan is one of the most abundant hemicelluloses and can crosslink cellulose and lignin to increase the stability of cell walls. A number of genes encoding glycosyltransferases play vital roles in xylan biosynthesis in plants, such as those of the GT43 family. However, little is...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zhen Li, Xinyue Wang, Kebin Yang, Chenglei Zhu, Tingting Yuan, Jiongliang Wang, Ying Li, Zhimin Gao
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Phyllostachys edulis Glycosyltransferase 43 MYB transcription factors Yeast one-hybrid Biotechnology TP248.13-248.65 Genetics QH426-470
Acceso en línea:	https://doaj.org/article/588a0cec79ce4b4b92f810af1841c7f2
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:588a0cec79ce4b4b92f810af1841c7f2
record_format	dspace
spelling	oai:doaj.org-article:588a0cec79ce4b4b92f810af1841c7f22021-12-05T12:17:11ZIdentification and expression analysis of the glycosyltransferase GT43 family members in bamboo reveal their potential function in xylan biosynthesis during rapid growth10.1186/s12864-021-08192-y1471-2164https://doaj.org/article/588a0cec79ce4b4b92f810af1841c7f22021-12-01T00:00:00Zhttps://doi.org/10.1186/s12864-021-08192-yhttps://doaj.org/toc/1471-2164Abstract Background Xylan is one of the most abundant hemicelluloses and can crosslink cellulose and lignin to increase the stability of cell walls. A number of genes encoding glycosyltransferases play vital roles in xylan biosynthesis in plants, such as those of the GT43 family. However, little is known about glycosyltransferases in bamboo, especially woody bamboo which is a good substitute for timber. Results A total of 17 GT43 genes (PeGT43–1 ~ PeGT43–17) were identified in the genome of moso bamboo (Phyllostachys edulis), which belong to three subfamilies with specific motifs. The phylogenetic and collinearity analyses showed that PeGT43s may have undergone gene duplication, as a result of collinearity found in 12 pairs of PeGT43s, and between 17 PeGT43s and 10 OsGT43s. A set of cis-acting elements such as hormones, abiotic stress response and MYB binding elements were found in the promoter of PeGT43s. PeGT43s were expressed differently in 26 tissues, among which the highest expression level was found in the shoots, especially in the rapid elongation zone and nodes. The genes coexpressed with PeGT43s were annotated as associated with polysaccharide metabolism and cell wall biosynthesis. qRT–PCR results showed that the coexpressed genes had similar expression patterns with a significant increase in 4.0 m shoots and a peak in 6.0 m shoots during fast growth. In addition, the xylan content and structural polysaccharide staining intensity in bamboo shoots showed a strong positive correlation with the expression of PeGT43s. Yeast one-hybrid assays demonstrated that PeMYB35 could recognize the 5′ UTR/promoter of PeGT43–5 by binding to the SMRE cis-elements. Conclusions PeGT43s were found to be adapted to the requirement of xylan biosynthesis during rapid cell elongation and cell wall accumulation, as evidenced by the expression profile of PeGT43s and the rate of xylan accumulation in bamboo shoots. Yeast one-hybrid analysis suggested that PeMYB35 might be involved in xylan biosynthesis by regulating the expression of PeGT43–5 by binding to its 5′ UTR/promoter. Our study provides a comprehensive understanding of PeGT43s in moso bamboo and lays a foundation for further functional analysis of PeGT43s for xylan biosynthesis during rapid growth.Zhen LiXinyue WangKebin YangChenglei ZhuTingting YuanJiongliang WangYing LiZhimin GaoBMCarticlePhyllostachys edulisGlycosyltransferase 43MYB transcription factorsYeast one-hybridBiotechnologyTP248.13-248.65GeneticsQH426-470ENBMC Genomics, Vol 22, Iss 1, Pp 1-18 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Phyllostachys edulis Glycosyltransferase 43 MYB transcription factors Yeast one-hybrid Biotechnology TP248.13-248.65 Genetics QH426-470
spellingShingle	Phyllostachys edulis Glycosyltransferase 43 MYB transcription factors Yeast one-hybrid Biotechnology TP248.13-248.65 Genetics QH426-470 Zhen Li Xinyue Wang Kebin Yang Chenglei Zhu Tingting Yuan Jiongliang Wang Ying Li Zhimin Gao Identification and expression analysis of the glycosyltransferase GT43 family members in bamboo reveal their potential function in xylan biosynthesis during rapid growth
description	Abstract Background Xylan is one of the most abundant hemicelluloses and can crosslink cellulose and lignin to increase the stability of cell walls. A number of genes encoding glycosyltransferases play vital roles in xylan biosynthesis in plants, such as those of the GT43 family. However, little is known about glycosyltransferases in bamboo, especially woody bamboo which is a good substitute for timber. Results A total of 17 GT43 genes (PeGT43–1 ~ PeGT43–17) were identified in the genome of moso bamboo (Phyllostachys edulis), which belong to three subfamilies with specific motifs. The phylogenetic and collinearity analyses showed that PeGT43s may have undergone gene duplication, as a result of collinearity found in 12 pairs of PeGT43s, and between 17 PeGT43s and 10 OsGT43s. A set of cis-acting elements such as hormones, abiotic stress response and MYB binding elements were found in the promoter of PeGT43s. PeGT43s were expressed differently in 26 tissues, among which the highest expression level was found in the shoots, especially in the rapid elongation zone and nodes. The genes coexpressed with PeGT43s were annotated as associated with polysaccharide metabolism and cell wall biosynthesis. qRT–PCR results showed that the coexpressed genes had similar expression patterns with a significant increase in 4.0 m shoots and a peak in 6.0 m shoots during fast growth. In addition, the xylan content and structural polysaccharide staining intensity in bamboo shoots showed a strong positive correlation with the expression of PeGT43s. Yeast one-hybrid assays demonstrated that PeMYB35 could recognize the 5′ UTR/promoter of PeGT43–5 by binding to the SMRE cis-elements. Conclusions PeGT43s were found to be adapted to the requirement of xylan biosynthesis during rapid cell elongation and cell wall accumulation, as evidenced by the expression profile of PeGT43s and the rate of xylan accumulation in bamboo shoots. Yeast one-hybrid analysis suggested that PeMYB35 might be involved in xylan biosynthesis by regulating the expression of PeGT43–5 by binding to its 5′ UTR/promoter. Our study provides a comprehensive understanding of PeGT43s in moso bamboo and lays a foundation for further functional analysis of PeGT43s for xylan biosynthesis during rapid growth.
format	article
author	Zhen Li Xinyue Wang Kebin Yang Chenglei Zhu Tingting Yuan Jiongliang Wang Ying Li Zhimin Gao
author_facet	Zhen Li Xinyue Wang Kebin Yang Chenglei Zhu Tingting Yuan Jiongliang Wang Ying Li Zhimin Gao
author_sort	Zhen Li
title	Identification and expression analysis of the glycosyltransferase GT43 family members in bamboo reveal their potential function in xylan biosynthesis during rapid growth
title_short	Identification and expression analysis of the glycosyltransferase GT43 family members in bamboo reveal their potential function in xylan biosynthesis during rapid growth
title_full	Identification and expression analysis of the glycosyltransferase GT43 family members in bamboo reveal their potential function in xylan biosynthesis during rapid growth
title_fullStr	Identification and expression analysis of the glycosyltransferase GT43 family members in bamboo reveal their potential function in xylan biosynthesis during rapid growth
title_full_unstemmed	Identification and expression analysis of the glycosyltransferase GT43 family members in bamboo reveal their potential function in xylan biosynthesis during rapid growth
title_sort	identification and expression analysis of the glycosyltransferase gt43 family members in bamboo reveal their potential function in xylan biosynthesis during rapid growth
publisher	BMC
publishDate	2021
url	https://doaj.org/article/588a0cec79ce4b4b92f810af1841c7f2
work_keys_str_mv	AT zhenli identificationandexpressionanalysisoftheglycosyltransferasegt43familymembersinbamboorevealtheirpotentialfunctioninxylanbiosynthesisduringrapidgrowth AT xinyuewang identificationandexpressionanalysisoftheglycosyltransferasegt43familymembersinbamboorevealtheirpotentialfunctioninxylanbiosynthesisduringrapidgrowth AT kebinyang identificationandexpressionanalysisoftheglycosyltransferasegt43familymembersinbamboorevealtheirpotentialfunctioninxylanbiosynthesisduringrapidgrowth AT chengleizhu identificationandexpressionanalysisoftheglycosyltransferasegt43familymembersinbamboorevealtheirpotentialfunctioninxylanbiosynthesisduringrapidgrowth AT tingtingyuan identificationandexpressionanalysisoftheglycosyltransferasegt43familymembersinbamboorevealtheirpotentialfunctioninxylanbiosynthesisduringrapidgrowth AT jiongliangwang identificationandexpressionanalysisoftheglycosyltransferasegt43familymembersinbamboorevealtheirpotentialfunctioninxylanbiosynthesisduringrapidgrowth AT yingli identificationandexpressionanalysisoftheglycosyltransferasegt43familymembersinbamboorevealtheirpotentialfunctioninxylanbiosynthesisduringrapidgrowth AT zhimingao identificationandexpressionanalysisoftheglycosyltransferasegt43familymembersinbamboorevealtheirpotentialfunctioninxylanbiosynthesisduringrapidgrowth
_version_	1718372074859266048

Identification and expression analysis of the glycosyltransferase GT43 family members in bamboo reveal their potential function in xylan biosynthesis during rapid growth

Ejemplares similares