Clusterin suppresses invasion and metastasis of testicular seminoma by upregulating COL15a1

Seminoma is the most common subtype of testicular germ cell tumor, with an increasing incidence worldwide. Clusterin (CLU) expression was found to be downregulated in testicular seminoma in our previous study. We now expanded the sample size, and further indicated that CLU expression correlates with...

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Autores principales:	Yankang Cui, Chenkui Miao, Shouyong Liu, Jingyuan Tang, Jing Zhang, Hengtao Bu, Yuhao Wang, Chao Liang, Meiling Bao, Chao Hou, Jiajin Wu, Xiaochao Chen, Xiang Zhang, Zengjun Wang, Bianjiang Liu
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	clusterin seminoma EMT COL15a1 MEF2A testicular xenografts in situ Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/5892ed501ea840abbc07b33ffcaf8bb9
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spelling	oai:doaj.org-article:5892ed501ea840abbc07b33ffcaf8bb92021-11-22T04:23:58ZClusterin suppresses invasion and metastasis of testicular seminoma by upregulating COL15a12162-253110.1016/j.omtn.2021.11.004https://doaj.org/article/5892ed501ea840abbc07b33ffcaf8bb92021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2162253121002791https://doaj.org/toc/2162-2531Seminoma is the most common subtype of testicular germ cell tumor, with an increasing incidence worldwide. Clusterin (CLU) expression was found to be downregulated in testicular seminoma in our previous study. We now expanded the sample size, and further indicated that CLU expression correlates with tumor stage. Tcam-2 cell line was used to investigate the CLU function in testicular seminoma, and CLU was found to inhibit the proliferation and metastasis abilities. Besides, extracellular matrix protein COL15a1 was demonstrated as the downstream of CLU to affect the epithelial-mesenchymal transition (EMT) process via competitively binding to DDR1 with COL1A1 and inhibiting the phosphorylation of PYK2. MEF2A was found to interact with CLU and bind to the promoter of COL15a1 and so upregulate its expression. This is the first study using testicular xenografts in situ to simulate testicular seminoma metastatic and proliferative capacities. In conclusion, CLU acts as a tumor suppressor to inhibit the metastasis of testicular seminoma by interacting with MEF2A to upregulate COL15a1 and blocking the EMT process.Yankang CuiChenkui MiaoShouyong LiuJingyuan TangJing ZhangHengtao BuYuhao WangChao LiangMeiling BaoChao HouJiajin WuXiaochao ChenXiang ZhangZengjun WangBianjiang LiuElsevierarticleclusterinseminomaEMTCOL15a1MEF2Atesticular xenografts in situTherapeutics. PharmacologyRM1-950ENMolecular Therapy: Nucleic Acids, Vol 26, Iss , Pp 1336-1350 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	clusterin seminoma EMT COL15a1 MEF2A testicular xenografts in situ Therapeutics. Pharmacology RM1-950
spellingShingle	clusterin seminoma EMT COL15a1 MEF2A testicular xenografts in situ Therapeutics. Pharmacology RM1-950 Yankang Cui Chenkui Miao Shouyong Liu Jingyuan Tang Jing Zhang Hengtao Bu Yuhao Wang Chao Liang Meiling Bao Chao Hou Jiajin Wu Xiaochao Chen Xiang Zhang Zengjun Wang Bianjiang Liu Clusterin suppresses invasion and metastasis of testicular seminoma by upregulating COL15a1
description	Seminoma is the most common subtype of testicular germ cell tumor, with an increasing incidence worldwide. Clusterin (CLU) expression was found to be downregulated in testicular seminoma in our previous study. We now expanded the sample size, and further indicated that CLU expression correlates with tumor stage. Tcam-2 cell line was used to investigate the CLU function in testicular seminoma, and CLU was found to inhibit the proliferation and metastasis abilities. Besides, extracellular matrix protein COL15a1 was demonstrated as the downstream of CLU to affect the epithelial-mesenchymal transition (EMT) process via competitively binding to DDR1 with COL1A1 and inhibiting the phosphorylation of PYK2. MEF2A was found to interact with CLU and bind to the promoter of COL15a1 and so upregulate its expression. This is the first study using testicular xenografts in situ to simulate testicular seminoma metastatic and proliferative capacities. In conclusion, CLU acts as a tumor suppressor to inhibit the metastasis of testicular seminoma by interacting with MEF2A to upregulate COL15a1 and blocking the EMT process.
format	article
author	Yankang Cui Chenkui Miao Shouyong Liu Jingyuan Tang Jing Zhang Hengtao Bu Yuhao Wang Chao Liang Meiling Bao Chao Hou Jiajin Wu Xiaochao Chen Xiang Zhang Zengjun Wang Bianjiang Liu
author_facet	Yankang Cui Chenkui Miao Shouyong Liu Jingyuan Tang Jing Zhang Hengtao Bu Yuhao Wang Chao Liang Meiling Bao Chao Hou Jiajin Wu Xiaochao Chen Xiang Zhang Zengjun Wang Bianjiang Liu
author_sort	Yankang Cui
title	Clusterin suppresses invasion and metastasis of testicular seminoma by upregulating COL15a1
title_short	Clusterin suppresses invasion and metastasis of testicular seminoma by upregulating COL15a1
title_full	Clusterin suppresses invasion and metastasis of testicular seminoma by upregulating COL15a1
title_fullStr	Clusterin suppresses invasion and metastasis of testicular seminoma by upregulating COL15a1
title_full_unstemmed	Clusterin suppresses invasion and metastasis of testicular seminoma by upregulating COL15a1
title_sort	clusterin suppresses invasion and metastasis of testicular seminoma by upregulating col15a1
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/5892ed501ea840abbc07b33ffcaf8bb9
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Clusterin suppresses invasion and metastasis of testicular seminoma by upregulating COL15a1

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