A high-frequency single nucleotide polymorphism in the MtrB sensor kinase in clinical strains of Mycobacterium tuberculosis alters its biochemical and physiological properties.

The DNA polymorphisms found in clinical strains of Mycobacterium tuberculosis drive altered physiology, virulence, and pathogenesis in them. Although the lineages of these clinical strains can be traced back to common ancestor/s, there exists a plethora of difference between them, compared to those...

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Autores principales:	Uchenna Watson Waturuocha, Athira P J, Krishna Kumar Singh, Vandana Malhotra, M S Krishna, Deepak Kumar Saini
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5894f90b3e9c471a9c37db473331f745
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spelling	oai:doaj.org-article:5894f90b3e9c471a9c37db473331f7452021-12-02T20:14:34ZA high-frequency single nucleotide polymorphism in the MtrB sensor kinase in clinical strains of Mycobacterium tuberculosis alters its biochemical and physiological properties.1932-620310.1371/journal.pone.0256664https://doaj.org/article/5894f90b3e9c471a9c37db473331f7452021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0256664https://doaj.org/toc/1932-6203The DNA polymorphisms found in clinical strains of Mycobacterium tuberculosis drive altered physiology, virulence, and pathogenesis in them. Although the lineages of these clinical strains can be traced back to common ancestor/s, there exists a plethora of difference between them, compared to those that have evolved in the laboratory. We identify a mutation present in ~80% of clinical strains, which maps in the HATPase domain of the sensor kinase MtrB and alters kinase and phosphatase activities, and affects its physiological role. The changes conferred by the mutation were probed by in-vitro biochemical assays which revealed changes in signaling properties of the sensor kinase. These changes also affect bacterial cell division rates, size and membrane properties. The study highlights the impact of DNA polymorphisms on the pathophysiology of clinical strains and provides insights into underlying mechanisms that drive signal transduction in pathogenic bacteria.Uchenna Watson WaturuochaAthira P JKrishna Kumar SinghVandana MalhotraM S KrishnaDeepak Kumar SainiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0256664 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Uchenna Watson Waturuocha Athira P J Krishna Kumar Singh Vandana Malhotra M S Krishna Deepak Kumar Saini A high-frequency single nucleotide polymorphism in the MtrB sensor kinase in clinical strains of Mycobacterium tuberculosis alters its biochemical and physiological properties.
description	The DNA polymorphisms found in clinical strains of Mycobacterium tuberculosis drive altered physiology, virulence, and pathogenesis in them. Although the lineages of these clinical strains can be traced back to common ancestor/s, there exists a plethora of difference between them, compared to those that have evolved in the laboratory. We identify a mutation present in ~80% of clinical strains, which maps in the HATPase domain of the sensor kinase MtrB and alters kinase and phosphatase activities, and affects its physiological role. The changes conferred by the mutation were probed by in-vitro biochemical assays which revealed changes in signaling properties of the sensor kinase. These changes also affect bacterial cell division rates, size and membrane properties. The study highlights the impact of DNA polymorphisms on the pathophysiology of clinical strains and provides insights into underlying mechanisms that drive signal transduction in pathogenic bacteria.
format	article
author	Uchenna Watson Waturuocha Athira P J Krishna Kumar Singh Vandana Malhotra M S Krishna Deepak Kumar Saini
author_facet	Uchenna Watson Waturuocha Athira P J Krishna Kumar Singh Vandana Malhotra M S Krishna Deepak Kumar Saini
author_sort	Uchenna Watson Waturuocha
title	A high-frequency single nucleotide polymorphism in the MtrB sensor kinase in clinical strains of Mycobacterium tuberculosis alters its biochemical and physiological properties.
title_short	A high-frequency single nucleotide polymorphism in the MtrB sensor kinase in clinical strains of Mycobacterium tuberculosis alters its biochemical and physiological properties.
title_full	A high-frequency single nucleotide polymorphism in the MtrB sensor kinase in clinical strains of Mycobacterium tuberculosis alters its biochemical and physiological properties.
title_fullStr	A high-frequency single nucleotide polymorphism in the MtrB sensor kinase in clinical strains of Mycobacterium tuberculosis alters its biochemical and physiological properties.
title_full_unstemmed	A high-frequency single nucleotide polymorphism in the MtrB sensor kinase in clinical strains of Mycobacterium tuberculosis alters its biochemical and physiological properties.
title_sort	high-frequency single nucleotide polymorphism in the mtrb sensor kinase in clinical strains of mycobacterium tuberculosis alters its biochemical and physiological properties.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/5894f90b3e9c471a9c37db473331f745
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A high-frequency single nucleotide polymorphism in the MtrB sensor kinase in clinical strains of Mycobacterium tuberculosis alters its biochemical and physiological properties.

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