A Yeast RNA-Interference Pesticide Targeting the <i>Irx</i> Gene Functions as a Broad-Based Mosquito Larvicide and Adulticide

Concerns for widespread insecticide resistance and the unintended impacts of insecticides on nontarget organisms have generated a pressing need for mosquito control innovations. A yeast RNAi-based insecticide that targets a conserved site in mosquito <i>Irx</i> family genes, but which ha...

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Autores principales:	Keshava Mysore, Longhua Sun, Limb K. Hapairai, Chien-Wei Wang, Jessica Igiede, Joseph B. Roethele, Nicholas D. Scheel, Max P. Scheel, Ping Li, Na Wei, David W. Severson, Molly Duman-Scheel
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Aedes albopictus</i> <i>Aedes aegypti</i> <i>Anopheles gambiae</i> ATSB <i>Culex quinquefasciatus</i> insecticide Science Q
Acceso en línea:	https://doaj.org/article/589cc181891a426eb59062ea88bcb4ba
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spelling	oai:doaj.org-article:589cc181891a426eb59062ea88bcb4ba2021-11-25T17:59:18ZA Yeast RNA-Interference Pesticide Targeting the <i>Irx</i> Gene Functions as a Broad-Based Mosquito Larvicide and Adulticide10.3390/insects121109862075-4450https://doaj.org/article/589cc181891a426eb59062ea88bcb4ba2021-11-01T00:00:00Zhttps://www.mdpi.com/2075-4450/12/11/986https://doaj.org/toc/2075-4450Concerns for widespread insecticide resistance and the unintended impacts of insecticides on nontarget organisms have generated a pressing need for mosquito control innovations. A yeast RNAi-based insecticide that targets a conserved site in mosquito <i>Irx</i> family genes, but which has not yet been identified in the genomes of nontarget organisms, was developed and characterized. <i>Saccharomyces cerevisiae</i> constructed to express short hairpin RNA (shRNA) matching the target site induced significant <i>Aedes aegypti</i> larval death in both lab trials and outdoor semi-field evaluations. The yeast also induced high levels of mortality in adult females, which readily consumed yeast incorporated into an attractive targeted sugar bait (ATSB) during simulated field trials. A conserved requirement for <i>Irx</i> function as a regulator of proneural gene expression was observed in the mosquito brain, suggesting a possible mode of action. The larvicidal and adulticidal properties of the yeast were also verified in <i>Aedes albopictus, Anopheles gambiae</i>, and <i>Culex</i><i>quinquefasciatus</i> mosquitoes, but the yeast larvicide was not toxic to other nontarget arthropods. These results indicate that further development and evaluation of this technology as an ecofriendly control intervention is warranted, and that ATSBs, an emerging mosquito control paradigm, could potentially be enriched through the use of yeast-based RNAi technology.Keshava MysoreLonghua SunLimb K. HapairaiChien-Wei WangJessica IgiedeJoseph B. RoetheleNicholas D. ScheelMax P. ScheelPing LiNa WeiDavid W. SeversonMolly Duman-ScheelMDPI AGarticle<i>Aedes albopictus</i><i>Aedes aegypti</i><i>Anopheles gambiae</i>ATSB<i>Culex quinquefasciatus</i>insecticideScienceQENInsects, Vol 12, Iss 986, p 986 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Aedes albopictus</i> <i>Aedes aegypti</i> <i>Anopheles gambiae</i> ATSB <i>Culex quinquefasciatus</i> insecticide Science Q
spellingShingle	<i>Aedes albopictus</i> <i>Aedes aegypti</i> <i>Anopheles gambiae</i> ATSB <i>Culex quinquefasciatus</i> insecticide Science Q Keshava Mysore Longhua Sun Limb K. Hapairai Chien-Wei Wang Jessica Igiede Joseph B. Roethele Nicholas D. Scheel Max P. Scheel Ping Li Na Wei David W. Severson Molly Duman-Scheel A Yeast RNA-Interference Pesticide Targeting the <i>Irx</i> Gene Functions as a Broad-Based Mosquito Larvicide and Adulticide
description	Concerns for widespread insecticide resistance and the unintended impacts of insecticides on nontarget organisms have generated a pressing need for mosquito control innovations. A yeast RNAi-based insecticide that targets a conserved site in mosquito <i>Irx</i> family genes, but which has not yet been identified in the genomes of nontarget organisms, was developed and characterized. <i>Saccharomyces cerevisiae</i> constructed to express short hairpin RNA (shRNA) matching the target site induced significant <i>Aedes aegypti</i> larval death in both lab trials and outdoor semi-field evaluations. The yeast also induced high levels of mortality in adult females, which readily consumed yeast incorporated into an attractive targeted sugar bait (ATSB) during simulated field trials. A conserved requirement for <i>Irx</i> function as a regulator of proneural gene expression was observed in the mosquito brain, suggesting a possible mode of action. The larvicidal and adulticidal properties of the yeast were also verified in <i>Aedes albopictus, Anopheles gambiae</i>, and <i>Culex</i><i>quinquefasciatus</i> mosquitoes, but the yeast larvicide was not toxic to other nontarget arthropods. These results indicate that further development and evaluation of this technology as an ecofriendly control intervention is warranted, and that ATSBs, an emerging mosquito control paradigm, could potentially be enriched through the use of yeast-based RNAi technology.
format	article
author	Keshava Mysore Longhua Sun Limb K. Hapairai Chien-Wei Wang Jessica Igiede Joseph B. Roethele Nicholas D. Scheel Max P. Scheel Ping Li Na Wei David W. Severson Molly Duman-Scheel
author_facet	Keshava Mysore Longhua Sun Limb K. Hapairai Chien-Wei Wang Jessica Igiede Joseph B. Roethele Nicholas D. Scheel Max P. Scheel Ping Li Na Wei David W. Severson Molly Duman-Scheel
author_sort	Keshava Mysore
title	A Yeast RNA-Interference Pesticide Targeting the <i>Irx</i> Gene Functions as a Broad-Based Mosquito Larvicide and Adulticide
title_short	A Yeast RNA-Interference Pesticide Targeting the <i>Irx</i> Gene Functions as a Broad-Based Mosquito Larvicide and Adulticide
title_full	A Yeast RNA-Interference Pesticide Targeting the <i>Irx</i> Gene Functions as a Broad-Based Mosquito Larvicide and Adulticide
title_fullStr	A Yeast RNA-Interference Pesticide Targeting the <i>Irx</i> Gene Functions as a Broad-Based Mosquito Larvicide and Adulticide
title_full_unstemmed	A Yeast RNA-Interference Pesticide Targeting the <i>Irx</i> Gene Functions as a Broad-Based Mosquito Larvicide and Adulticide
title_sort	yeast rna-interference pesticide targeting the <i>irx</i> gene functions as a broad-based mosquito larvicide and adulticide
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/589cc181891a426eb59062ea88bcb4ba
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A Yeast RNA-Interference Pesticide Targeting the <i>Irx</i> Gene Functions as a Broad-Based Mosquito Larvicide and Adulticide

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