The effects of bone marrow stem and progenitor cell seeding on urinary bladder tissue regeneration

Abstract Complications associated with urinary bladder augmentation provide the motivation to delineate alternative bladder tissue regenerative engineering strategies. We describe the results of varying the proportion of bone marrow (BM) mesenchymal stem cells (MSCs) to CD34 + hematopoietic stem/pro...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Matthew I. Bury, Natalie J. Fuller, Renea M. Sturm, Rebecca R. Rabizadeh, Bonnie G. Nolan, Milica Barac, Sonia S. Edassery, Yvonne Y. Chan, Arun K. Sharma
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/58a564ed935b4d34be98b940765bb453
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:58a564ed935b4d34be98b940765bb453
record_format	dspace
spelling	oai:doaj.org-article:58a564ed935b4d34be98b940765bb4532021-12-02T10:48:03ZThe effects of bone marrow stem and progenitor cell seeding on urinary bladder tissue regeneration10.1038/s41598-021-81939-52045-2322https://doaj.org/article/58a564ed935b4d34be98b940765bb4532021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81939-5https://doaj.org/toc/2045-2322Abstract Complications associated with urinary bladder augmentation provide the motivation to delineate alternative bladder tissue regenerative engineering strategies. We describe the results of varying the proportion of bone marrow (BM) mesenchymal stem cells (MSCs) to CD34 + hematopoietic stem/progenitor cells (HSPCs) co-seeded onto synthetic POC [poly(1,8 octamethylene citrate)] or small intestinal submucosa (SIS) scaffolds and their contribution to bladder tissue regeneration. Human BM MSCs and CD34 + HSPCs were co-seeded onto POC or SIS scaffolds at cell ratios of 50 K CD34 + HSPCs/15 K MSCs (CD34-50/MSC15); 50 K CD34 + HSPCs/30 K MSCs (CD34-50/MSC30); 100 K CD34 + HSPCs/15 K MSCs (CD34-100/MSC15); and 100 K CD34 + HSPCs/30 K MSCs (CD34-100/MSC30), in male (M/POC; M/SIS; n = 6/cell seeded scaffold) and female (F/POC; F/SIS; n = 6/cell seeded scaffold) nude rats (n = 96 total animals). Explanted scaffold/composite augmented bladder tissue underwent quantitative morphometrics following histological staining taking into account the presence (S+) or absence (S−) of bladder stones. Urodynamic studies were also performed. Regarding regenerated tissue vascularization, an upward shift was detected for some higher seeded density groups including the CD34-100/MSC30 groups [F/POC S− CD34-100/MSC30 230.5 ± 12.4; F/POC S+ CD34-100/MSC30 245.6 ± 23.4; F/SIS S+ CD34-100/MSC30 278.1; F/SIS S− CD34-100/MSC30 187.4 ± 8.1; (vessels/mm2)]. Similarly, a potential trend toward increased levels of percent muscle (≥ 45% muscle) with higher seeding densities was observed for F/POC S− [CD34-50/MSC30 48.8 ± 2.2; CD34-100/MSC15 53.9 ± 2.8; CD34-100/MSC30 50.7 ± 1.7] and for F/SIS S− [CD34-100/MSC15 47.1 ± 1.6; CD34-100/MSC30 51.2 ± 2.3]. As a potential trend, higher MSC/CD34 + HSPCs cell seeding densities generally tended to increase levels of tissue vascularization and aided with bladder muscle growth. Data suggest that increasing cell seeding density has the potential to enhance bladder tissue regeneration in our model.Matthew I. BuryNatalie J. FullerRenea M. SturmRebecca R. RabizadehBonnie G. NolanMilica BaracSonia S. EdasseryYvonne Y. ChanArun K. SharmaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Matthew I. Bury Natalie J. Fuller Renea M. Sturm Rebecca R. Rabizadeh Bonnie G. Nolan Milica Barac Sonia S. Edassery Yvonne Y. Chan Arun K. Sharma The effects of bone marrow stem and progenitor cell seeding on urinary bladder tissue regeneration
description	Abstract Complications associated with urinary bladder augmentation provide the motivation to delineate alternative bladder tissue regenerative engineering strategies. We describe the results of varying the proportion of bone marrow (BM) mesenchymal stem cells (MSCs) to CD34 + hematopoietic stem/progenitor cells (HSPCs) co-seeded onto synthetic POC [poly(1,8 octamethylene citrate)] or small intestinal submucosa (SIS) scaffolds and their contribution to bladder tissue regeneration. Human BM MSCs and CD34 + HSPCs were co-seeded onto POC or SIS scaffolds at cell ratios of 50 K CD34 + HSPCs/15 K MSCs (CD34-50/MSC15); 50 K CD34 + HSPCs/30 K MSCs (CD34-50/MSC30); 100 K CD34 + HSPCs/15 K MSCs (CD34-100/MSC15); and 100 K CD34 + HSPCs/30 K MSCs (CD34-100/MSC30), in male (M/POC; M/SIS; n = 6/cell seeded scaffold) and female (F/POC; F/SIS; n = 6/cell seeded scaffold) nude rats (n = 96 total animals). Explanted scaffold/composite augmented bladder tissue underwent quantitative morphometrics following histological staining taking into account the presence (S+) or absence (S−) of bladder stones. Urodynamic studies were also performed. Regarding regenerated tissue vascularization, an upward shift was detected for some higher seeded density groups including the CD34-100/MSC30 groups [F/POC S− CD34-100/MSC30 230.5 ± 12.4; F/POC S+ CD34-100/MSC30 245.6 ± 23.4; F/SIS S+ CD34-100/MSC30 278.1; F/SIS S− CD34-100/MSC30 187.4 ± 8.1; (vessels/mm2)]. Similarly, a potential trend toward increased levels of percent muscle (≥ 45% muscle) with higher seeding densities was observed for F/POC S− [CD34-50/MSC30 48.8 ± 2.2; CD34-100/MSC15 53.9 ± 2.8; CD34-100/MSC30 50.7 ± 1.7] and for F/SIS S− [CD34-100/MSC15 47.1 ± 1.6; CD34-100/MSC30 51.2 ± 2.3]. As a potential trend, higher MSC/CD34 + HSPCs cell seeding densities generally tended to increase levels of tissue vascularization and aided with bladder muscle growth. Data suggest that increasing cell seeding density has the potential to enhance bladder tissue regeneration in our model.
format	article
author	Matthew I. Bury Natalie J. Fuller Renea M. Sturm Rebecca R. Rabizadeh Bonnie G. Nolan Milica Barac Sonia S. Edassery Yvonne Y. Chan Arun K. Sharma
author_facet	Matthew I. Bury Natalie J. Fuller Renea M. Sturm Rebecca R. Rabizadeh Bonnie G. Nolan Milica Barac Sonia S. Edassery Yvonne Y. Chan Arun K. Sharma
author_sort	Matthew I. Bury
title	The effects of bone marrow stem and progenitor cell seeding on urinary bladder tissue regeneration
title_short	The effects of bone marrow stem and progenitor cell seeding on urinary bladder tissue regeneration
title_full	The effects of bone marrow stem and progenitor cell seeding on urinary bladder tissue regeneration
title_fullStr	The effects of bone marrow stem and progenitor cell seeding on urinary bladder tissue regeneration
title_full_unstemmed	The effects of bone marrow stem and progenitor cell seeding on urinary bladder tissue regeneration
title_sort	effects of bone marrow stem and progenitor cell seeding on urinary bladder tissue regeneration
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/58a564ed935b4d34be98b940765bb453
work_keys_str_mv	AT matthewibury theeffectsofbonemarrowstemandprogenitorcellseedingonurinarybladdertissueregeneration AT nataliejfuller theeffectsofbonemarrowstemandprogenitorcellseedingonurinarybladdertissueregeneration AT reneamsturm theeffectsofbonemarrowstemandprogenitorcellseedingonurinarybladdertissueregeneration AT rebeccarrabizadeh theeffectsofbonemarrowstemandprogenitorcellseedingonurinarybladdertissueregeneration AT bonniegnolan theeffectsofbonemarrowstemandprogenitorcellseedingonurinarybladdertissueregeneration AT milicabarac theeffectsofbonemarrowstemandprogenitorcellseedingonurinarybladdertissueregeneration AT soniasedassery theeffectsofbonemarrowstemandprogenitorcellseedingonurinarybladdertissueregeneration AT yvonneychan theeffectsofbonemarrowstemandprogenitorcellseedingonurinarybladdertissueregeneration AT arunksharma theeffectsofbonemarrowstemandprogenitorcellseedingonurinarybladdertissueregeneration AT matthewibury effectsofbonemarrowstemandprogenitorcellseedingonurinarybladdertissueregeneration AT nataliejfuller effectsofbonemarrowstemandprogenitorcellseedingonurinarybladdertissueregeneration AT reneamsturm effectsofbonemarrowstemandprogenitorcellseedingonurinarybladdertissueregeneration AT rebeccarrabizadeh effectsofbonemarrowstemandprogenitorcellseedingonurinarybladdertissueregeneration AT bonniegnolan effectsofbonemarrowstemandprogenitorcellseedingonurinarybladdertissueregeneration AT milicabarac effectsofbonemarrowstemandprogenitorcellseedingonurinarybladdertissueregeneration AT soniasedassery effectsofbonemarrowstemandprogenitorcellseedingonurinarybladdertissueregeneration AT yvonneychan effectsofbonemarrowstemandprogenitorcellseedingonurinarybladdertissueregeneration AT arunksharma effectsofbonemarrowstemandprogenitorcellseedingonurinarybladdertissueregeneration
_version_	1718396646849511424

The effects of bone marrow stem and progenitor cell seeding on urinary bladder tissue regeneration

Ejemplares similares