Disparate subcellular location of putative sortase substrates in Clostridium difficile

Abstract Clostridium difficile is a gastrointestinal pathogen but how the bacterium colonises this niche is still little understood. Sortase enzymes covalently attach specific bacterial proteins to the peptidoglycan cell wall and are often involved in colonisation by pathogens. Here we show C. diffi...

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Autores principales:	Johann Peltier, Helen A. Shaw, Brendan W. Wren, Neil F. Fairweather
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/58afa41b9fab4de785c328b5caf7e816
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spelling	oai:doaj.org-article:58afa41b9fab4de785c328b5caf7e8162021-12-02T15:05:43ZDisparate subcellular location of putative sortase substrates in Clostridium difficile10.1038/s41598-017-08322-12045-2322https://doaj.org/article/58afa41b9fab4de785c328b5caf7e8162017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08322-1https://doaj.org/toc/2045-2322Abstract Clostridium difficile is a gastrointestinal pathogen but how the bacterium colonises this niche is still little understood. Sortase enzymes covalently attach specific bacterial proteins to the peptidoglycan cell wall and are often involved in colonisation by pathogens. Here we show C. difficile proteins CD2537 and CD3392 are functional substrates of sortase SrtB. Through manipulation of the C-terminal regions of these proteins we show the SPKTG motif is essential for covalent attachment to the cell wall. Two additional putative substrates, CD0183 which contains an SPSTG motif, and CD2768 which contains an SPQTG motif, are not cleaved or anchored to the cell wall by sortase. Finally, using an in vivo asymmetric cleavage assay, we show that despite containing a conserved SPKTG motif, in the absence of SrtB these proteins are localised to disparate cellular compartments.Johann PeltierHelen A. ShawBrendan W. WrenNeil F. FairweatherNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Johann Peltier Helen A. Shaw Brendan W. Wren Neil F. Fairweather Disparate subcellular location of putative sortase substrates in Clostridium difficile
description	Abstract Clostridium difficile is a gastrointestinal pathogen but how the bacterium colonises this niche is still little understood. Sortase enzymes covalently attach specific bacterial proteins to the peptidoglycan cell wall and are often involved in colonisation by pathogens. Here we show C. difficile proteins CD2537 and CD3392 are functional substrates of sortase SrtB. Through manipulation of the C-terminal regions of these proteins we show the SPKTG motif is essential for covalent attachment to the cell wall. Two additional putative substrates, CD0183 which contains an SPSTG motif, and CD2768 which contains an SPQTG motif, are not cleaved or anchored to the cell wall by sortase. Finally, using an in vivo asymmetric cleavage assay, we show that despite containing a conserved SPKTG motif, in the absence of SrtB these proteins are localised to disparate cellular compartments.
format	article
author	Johann Peltier Helen A. Shaw Brendan W. Wren Neil F. Fairweather
author_facet	Johann Peltier Helen A. Shaw Brendan W. Wren Neil F. Fairweather
author_sort	Johann Peltier
title	Disparate subcellular location of putative sortase substrates in Clostridium difficile
title_short	Disparate subcellular location of putative sortase substrates in Clostridium difficile
title_full	Disparate subcellular location of putative sortase substrates in Clostridium difficile
title_fullStr	Disparate subcellular location of putative sortase substrates in Clostridium difficile
title_full_unstemmed	Disparate subcellular location of putative sortase substrates in Clostridium difficile
title_sort	disparate subcellular location of putative sortase substrates in clostridium difficile
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/58afa41b9fab4de785c328b5caf7e816
work_keys_str_mv	AT johannpeltier disparatesubcellularlocationofputativesortasesubstratesinclostridiumdifficile AT helenashaw disparatesubcellularlocationofputativesortasesubstratesinclostridiumdifficile AT brendanwwren disparatesubcellularlocationofputativesortasesubstratesinclostridiumdifficile AT neilffairweather disparatesubcellularlocationofputativesortasesubstratesinclostridiumdifficile
_version_	1718388705801011200

Disparate subcellular location of putative sortase substrates in Clostridium difficile

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