Profiling temporal dynamics of acetogenic communities in anaerobic digesters using next-generation sequencing and T-RFLP

Abstract Acetogens play a key role in anaerobic degradation of organic material and in maintaining biogas process efficiency. Profiling this community and its temporal changes can help evaluate process stability and function, especially under disturbance/stress conditions, and avoid complete process...

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Autores principales:	Abhijeet Singh, Bettina Müller, Anna Schnürer
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/58b5e5768e224d91a719dea01aa7db06
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spelling	oai:doaj.org-article:58b5e5768e224d91a719dea01aa7db062021-12-02T16:05:54ZProfiling temporal dynamics of acetogenic communities in anaerobic digesters using next-generation sequencing and T-RFLP10.1038/s41598-021-92658-22045-2322https://doaj.org/article/58b5e5768e224d91a719dea01aa7db062021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92658-2https://doaj.org/toc/2045-2322Abstract Acetogens play a key role in anaerobic degradation of organic material and in maintaining biogas process efficiency. Profiling this community and its temporal changes can help evaluate process stability and function, especially under disturbance/stress conditions, and avoid complete process failure. The formyltetrahydrofolate synthetase (FTHFS) gene can be used as a marker for acetogenic community profiling in diverse environments. In this study, we developed a new high-throughput FTHFS gene sequencing method for acetogenic community profiling and compared it with conventional terminal restriction fragment length polymorphism of the FTHFS gene, 16S rRNA gene-based profiling of the whole bacterial community, and indirect analysis via 16S rRNA profiling of the FTHFS gene-harbouring community. Analyses and method comparisons were made using samples from two laboratory-scale biogas processes, one operated under stable control and one exposed to controlled overloading disturbance. Comparative analysis revealed satisfactory detection of the bacterial community and its changes for all methods, but with some differences in resolution and taxonomic identification. FTHFS gene sequencing was found to be the most suitable and reliable method to study acetogenic communities. These results pave the way for community profiling in various biogas processes and in other environments where the dynamics of acetogenic bacteria have not been well studied.Abhijeet SinghBettina MüllerAnna SchnürerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Abhijeet Singh Bettina Müller Anna Schnürer Profiling temporal dynamics of acetogenic communities in anaerobic digesters using next-generation sequencing and T-RFLP
description	Abstract Acetogens play a key role in anaerobic degradation of organic material and in maintaining biogas process efficiency. Profiling this community and its temporal changes can help evaluate process stability and function, especially under disturbance/stress conditions, and avoid complete process failure. The formyltetrahydrofolate synthetase (FTHFS) gene can be used as a marker for acetogenic community profiling in diverse environments. In this study, we developed a new high-throughput FTHFS gene sequencing method for acetogenic community profiling and compared it with conventional terminal restriction fragment length polymorphism of the FTHFS gene, 16S rRNA gene-based profiling of the whole bacterial community, and indirect analysis via 16S rRNA profiling of the FTHFS gene-harbouring community. Analyses and method comparisons were made using samples from two laboratory-scale biogas processes, one operated under stable control and one exposed to controlled overloading disturbance. Comparative analysis revealed satisfactory detection of the bacterial community and its changes for all methods, but with some differences in resolution and taxonomic identification. FTHFS gene sequencing was found to be the most suitable and reliable method to study acetogenic communities. These results pave the way for community profiling in various biogas processes and in other environments where the dynamics of acetogenic bacteria have not been well studied.
format	article
author	Abhijeet Singh Bettina Müller Anna Schnürer
author_facet	Abhijeet Singh Bettina Müller Anna Schnürer
author_sort	Abhijeet Singh
title	Profiling temporal dynamics of acetogenic communities in anaerobic digesters using next-generation sequencing and T-RFLP
title_short	Profiling temporal dynamics of acetogenic communities in anaerobic digesters using next-generation sequencing and T-RFLP
title_full	Profiling temporal dynamics of acetogenic communities in anaerobic digesters using next-generation sequencing and T-RFLP
title_fullStr	Profiling temporal dynamics of acetogenic communities in anaerobic digesters using next-generation sequencing and T-RFLP
title_full_unstemmed	Profiling temporal dynamics of acetogenic communities in anaerobic digesters using next-generation sequencing and T-RFLP
title_sort	profiling temporal dynamics of acetogenic communities in anaerobic digesters using next-generation sequencing and t-rflp
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/58b5e5768e224d91a719dea01aa7db06
work_keys_str_mv	AT abhijeetsingh profilingtemporaldynamicsofacetogeniccommunitiesinanaerobicdigestersusingnextgenerationsequencingandtrflp AT bettinamuller profilingtemporaldynamicsofacetogeniccommunitiesinanaerobicdigestersusingnextgenerationsequencingandtrflp AT annaschnurer profilingtemporaldynamicsofacetogeniccommunitiesinanaerobicdigestersusingnextgenerationsequencingandtrflp
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Profiling temporal dynamics of acetogenic communities in anaerobic digesters using next-generation sequencing and T-RFLP

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