Chitosan Films for Microfluidic Studies of Single Bacteria and Perspectives for Antibiotic Susceptibility Testing

ABSTRACT Single-cell microfluidics is a powerful method to study bacteria and determine their susceptibility to antibiotic treatment. Glass treatment by adhesive molecules is a potential solution to immobilize bacterial cells and perform microscopy, but traditional cationic polymers such as polylysi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Julie Tréguier, Loic Bugnicourt, Guillaume Gay, Mamoudou Diallo, Salim Timo Islam, Alexandre Toro, Laurent David, Olivier Théodoly, Guillaume Sudre, Tâm Mignot
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2019
Materias:	antibiotic susceptibility testing chitosan Escherichia coli Klebsiella pneumonia single cells microfluidics Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/58b6361f9ac54f8a8d385d0b1cce2513
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:58b6361f9ac54f8a8d385d0b1cce2513
record_format	dspace
spelling	oai:doaj.org-article:58b6361f9ac54f8a8d385d0b1cce25132021-11-15T16:22:09ZChitosan Films for Microfluidic Studies of Single Bacteria and Perspectives for Antibiotic Susceptibility Testing10.1128/mBio.01375-192150-7511https://doaj.org/article/58b6361f9ac54f8a8d385d0b1cce25132019-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01375-19https://doaj.org/toc/2150-7511ABSTRACT Single-cell microfluidics is a powerful method to study bacteria and determine their susceptibility to antibiotic treatment. Glass treatment by adhesive molecules is a potential solution to immobilize bacterial cells and perform microscopy, but traditional cationic polymers such as polylysine deeply affect bacterial physiology. In this work, we chemically characterized a class of chitosan polymers for their biocompatibility when adsorbed to glass. Chitosan chains of known length and composition allowed growth of Escherichia coli cells without any deleterious effects on cell physiology. Combined with a machine learning approach, this method could measure the antibiotic susceptibility of a diversity of clinical strains in less than 1 h and with higher accuracy than current methods. Finally, chitosan polymers also supported growth of Klebsiella pneumoniae, another bacterial pathogen of clinical significance. IMPORTANCE Current microfluidic techniques are powerful to study bacteria and determine their response to antibiotic treatment, but they are currently limited by their complex manipulation. Chitosan films are fully biocompatible and could thus be a viable replacement for existing commercial devices that currently use polylysine. Thus, the low cost of chitosan slides and their simple implementation make them highly versatile for research as well as clinical use.Julie TréguierLoic BugnicourtGuillaume GayMamoudou DialloSalim Timo IslamAlexandre ToroLaurent DavidOlivier ThéodolyGuillaume SudreTâm MignotAmerican Society for Microbiologyarticleantibiotic susceptibility testingchitosanEscherichia coliKlebsiella pneumoniasingle cellsmicrofluidicsMicrobiologyQR1-502ENmBio, Vol 10, Iss 4 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	antibiotic susceptibility testing chitosan Escherichia coli Klebsiella pneumonia single cells microfluidics Microbiology QR1-502
spellingShingle	antibiotic susceptibility testing chitosan Escherichia coli Klebsiella pneumonia single cells microfluidics Microbiology QR1-502 Julie Tréguier Loic Bugnicourt Guillaume Gay Mamoudou Diallo Salim Timo Islam Alexandre Toro Laurent David Olivier Théodoly Guillaume Sudre Tâm Mignot Chitosan Films for Microfluidic Studies of Single Bacteria and Perspectives for Antibiotic Susceptibility Testing
description	ABSTRACT Single-cell microfluidics is a powerful method to study bacteria and determine their susceptibility to antibiotic treatment. Glass treatment by adhesive molecules is a potential solution to immobilize bacterial cells and perform microscopy, but traditional cationic polymers such as polylysine deeply affect bacterial physiology. In this work, we chemically characterized a class of chitosan polymers for their biocompatibility when adsorbed to glass. Chitosan chains of known length and composition allowed growth of Escherichia coli cells without any deleterious effects on cell physiology. Combined with a machine learning approach, this method could measure the antibiotic susceptibility of a diversity of clinical strains in less than 1 h and with higher accuracy than current methods. Finally, chitosan polymers also supported growth of Klebsiella pneumoniae, another bacterial pathogen of clinical significance. IMPORTANCE Current microfluidic techniques are powerful to study bacteria and determine their response to antibiotic treatment, but they are currently limited by their complex manipulation. Chitosan films are fully biocompatible and could thus be a viable replacement for existing commercial devices that currently use polylysine. Thus, the low cost of chitosan slides and their simple implementation make them highly versatile for research as well as clinical use.
format	article
author	Julie Tréguier Loic Bugnicourt Guillaume Gay Mamoudou Diallo Salim Timo Islam Alexandre Toro Laurent David Olivier Théodoly Guillaume Sudre Tâm Mignot
author_facet	Julie Tréguier Loic Bugnicourt Guillaume Gay Mamoudou Diallo Salim Timo Islam Alexandre Toro Laurent David Olivier Théodoly Guillaume Sudre Tâm Mignot
author_sort	Julie Tréguier
title	Chitosan Films for Microfluidic Studies of Single Bacteria and Perspectives for Antibiotic Susceptibility Testing
title_short	Chitosan Films for Microfluidic Studies of Single Bacteria and Perspectives for Antibiotic Susceptibility Testing
title_full	Chitosan Films for Microfluidic Studies of Single Bacteria and Perspectives for Antibiotic Susceptibility Testing
title_fullStr	Chitosan Films for Microfluidic Studies of Single Bacteria and Perspectives for Antibiotic Susceptibility Testing
title_full_unstemmed	Chitosan Films for Microfluidic Studies of Single Bacteria and Perspectives for Antibiotic Susceptibility Testing
title_sort	chitosan films for microfluidic studies of single bacteria and perspectives for antibiotic susceptibility testing
publisher	American Society for Microbiology
publishDate	2019
url	https://doaj.org/article/58b6361f9ac54f8a8d385d0b1cce2513
work_keys_str_mv	AT julietreguier chitosanfilmsformicrofluidicstudiesofsinglebacteriaandperspectivesforantibioticsusceptibilitytesting AT loicbugnicourt chitosanfilmsformicrofluidicstudiesofsinglebacteriaandperspectivesforantibioticsusceptibilitytesting AT guillaumegay chitosanfilmsformicrofluidicstudiesofsinglebacteriaandperspectivesforantibioticsusceptibilitytesting AT mamoudoudiallo chitosanfilmsformicrofluidicstudiesofsinglebacteriaandperspectivesforantibioticsusceptibilitytesting AT salimtimoislam chitosanfilmsformicrofluidicstudiesofsinglebacteriaandperspectivesforantibioticsusceptibilitytesting AT alexandretoro chitosanfilmsformicrofluidicstudiesofsinglebacteriaandperspectivesforantibioticsusceptibilitytesting AT laurentdavid chitosanfilmsformicrofluidicstudiesofsinglebacteriaandperspectivesforantibioticsusceptibilitytesting AT oliviertheodoly chitosanfilmsformicrofluidicstudiesofsinglebacteriaandperspectivesforantibioticsusceptibilitytesting AT guillaumesudre chitosanfilmsformicrofluidicstudiesofsinglebacteriaandperspectivesforantibioticsusceptibilitytesting AT tammignot chitosanfilmsformicrofluidicstudiesofsinglebacteriaandperspectivesforantibioticsusceptibilitytesting
_version_	1718426903052812288

Chitosan Films for Microfluidic Studies of Single Bacteria and Perspectives for Antibiotic Susceptibility Testing

Ejemplares similares