MiCas9 increases large size gene knock-in rates and reduces undesirable on-target and off-target indel edits

Cas9 fused to DNA damage repair proteins can improve rates of gene knock-in but the chimeric protein is often large. Here the authors fuse Cas9 to a minimal Brex27 motif from BRCA2 consisting of thirty-six amino acids to enhance the efficacy and safety of gene editing.

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Detalles Bibliográficos
Autores principales:	Linyuan Ma, Jinxue Ruan, Jun Song, Luan Wen, Dongshan Yang, Jiangyang Zhao, Xiaofeng Xia, Y. Eugene Chen, Jifeng Zhang, Jie Xu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/58d62b4781c84e8aa9e7b4d0d8b4512d
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spelling	oai:doaj.org-article:58d62b4781c84e8aa9e7b4d0d8b4512d2021-12-02T15:39:16ZMiCas9 increases large size gene knock-in rates and reduces undesirable on-target and off-target indel edits10.1038/s41467-020-19842-22041-1723https://doaj.org/article/58d62b4781c84e8aa9e7b4d0d8b4512d2020-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-19842-2https://doaj.org/toc/2041-1723Cas9 fused to DNA damage repair proteins can improve rates of gene knock-in but the chimeric protein is often large. Here the authors fuse Cas9 to a minimal Brex27 motif from BRCA2 consisting of thirty-six amino acids to enhance the efficacy and safety of gene editing.Linyuan MaJinxue RuanJun SongLuan WenDongshan YangJiangyang ZhaoXiaofeng XiaY. Eugene ChenJifeng ZhangJie XuNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Linyuan Ma Jinxue Ruan Jun Song Luan Wen Dongshan Yang Jiangyang Zhao Xiaofeng Xia Y. Eugene Chen Jifeng Zhang Jie Xu MiCas9 increases large size gene knock-in rates and reduces undesirable on-target and off-target indel edits
description	Cas9 fused to DNA damage repair proteins can improve rates of gene knock-in but the chimeric protein is often large. Here the authors fuse Cas9 to a minimal Brex27 motif from BRCA2 consisting of thirty-six amino acids to enhance the efficacy and safety of gene editing.
format	article
author	Linyuan Ma Jinxue Ruan Jun Song Luan Wen Dongshan Yang Jiangyang Zhao Xiaofeng Xia Y. Eugene Chen Jifeng Zhang Jie Xu
author_facet	Linyuan Ma Jinxue Ruan Jun Song Luan Wen Dongshan Yang Jiangyang Zhao Xiaofeng Xia Y. Eugene Chen Jifeng Zhang Jie Xu
author_sort	Linyuan Ma
title	MiCas9 increases large size gene knock-in rates and reduces undesirable on-target and off-target indel edits
title_short	MiCas9 increases large size gene knock-in rates and reduces undesirable on-target and off-target indel edits
title_full	MiCas9 increases large size gene knock-in rates and reduces undesirable on-target and off-target indel edits
title_fullStr	MiCas9 increases large size gene knock-in rates and reduces undesirable on-target and off-target indel edits
title_full_unstemmed	MiCas9 increases large size gene knock-in rates and reduces undesirable on-target and off-target indel edits
title_sort	micas9 increases large size gene knock-in rates and reduces undesirable on-target and off-target indel edits
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/58d62b4781c84e8aa9e7b4d0d8b4512d
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MiCas9 increases large size gene knock-in rates and reduces undesirable on-target and off-target indel edits

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