The immune cell composition in Barrett's metaplastic tissue resembles that in normal duodenal tissue.

<h4>Background and objective</h4>Barrett's esophagus (BE) is characterized by the transition of squamous epithelium into columnar epithelium with intestinal metaplasia. The increased number and types of immune cells in BE have been indicated to be due to a Th2-type inflammatory proc...

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Autores principales:	Alexandra Lind, Peter D Siersema, Johannes G Kusters, Jan A M Van der Linden, Edward F Knol, Leo Koenderman
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/58e78c71269f4c8482975c5772b74c43
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spelling	oai:doaj.org-article:58e78c71269f4c8482975c5772b74c432021-11-18T07:23:24ZThe immune cell composition in Barrett's metaplastic tissue resembles that in normal duodenal tissue.1932-620310.1371/journal.pone.0033899https://doaj.org/article/58e78c71269f4c8482975c5772b74c432012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22509265/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background and objective</h4>Barrett's esophagus (BE) is characterized by the transition of squamous epithelium into columnar epithelium with intestinal metaplasia. The increased number and types of immune cells in BE have been indicated to be due to a Th2-type inflammatory process. We tested the alternative hypothesis that the abundance of T-cells in BE is caused by a homing mechanism that is found in the duodenum.<h4>Patients and methods</h4>Biopsies from BE and duodenal tissue from 30 BE patients and duodenal tissue from 18 controls were characterized by immmunohistochemistry for the presence of T-cells and eosinophils(eos). Ex vivo expanded T-cells were further phenotyped by multicolor analysis using flowcytometry.<h4>Results</h4>The high percentage of CD4(+)-T cells (69±3% (mean±SEM/n = 17, by flowcytometry)), measured by flowcytometry and immunohistochemistry, and the presence of non-activated eosinophils found in BE by immunohistochemical staining, were not different from that found in duodenal tissue. Expanded lymphocytes from these tissues had a similar phenotype, characterized by a comparable but low percentage of αE(CD103) positive CD4(+)cells (44±5% in BE, 43±4% in duodenum of BE and 34±7% in duodenum of controls) and a similar percentage of granzyme-B(+)CD8(+) cells(44±5% in BE, 33±6% in duodenum of BE and 36±7% in duodenum of controls). In addition, a similar percentage of α4β7(+) T-lymphocytes (63±5% in BE, 58±5% in duodenum of BE and 62±8% in duodenum of controls) was found. Finally, mRNA expression of the ligand for α4β7, MAdCAM-1, was also similar in BE and duodenal tissue. No evidence for a Th2-response was found as almost no IL-4(+)-T-cells were seen.<h4>Conclusion</h4>The immune cell composition (lymphocytes and eosinophils) and expression of intestinal adhesion molecule MAdCAM-1 is similar in BE and duodenum. This supports the hypothesis that homing of lymphocytes to BE tissue is mainly caused by intestinal homing signals rather than to an active inflammatory response.Alexandra LindPeter D SiersemaJohannes G KustersJan A M Van der LindenEdward F KnolLeo KoendermanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 4, p e33899 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alexandra Lind Peter D Siersema Johannes G Kusters Jan A M Van der Linden Edward F Knol Leo Koenderman The immune cell composition in Barrett's metaplastic tissue resembles that in normal duodenal tissue.
description	<h4>Background and objective</h4>Barrett's esophagus (BE) is characterized by the transition of squamous epithelium into columnar epithelium with intestinal metaplasia. The increased number and types of immune cells in BE have been indicated to be due to a Th2-type inflammatory process. We tested the alternative hypothesis that the abundance of T-cells in BE is caused by a homing mechanism that is found in the duodenum.<h4>Patients and methods</h4>Biopsies from BE and duodenal tissue from 30 BE patients and duodenal tissue from 18 controls were characterized by immmunohistochemistry for the presence of T-cells and eosinophils(eos). Ex vivo expanded T-cells were further phenotyped by multicolor analysis using flowcytometry.<h4>Results</h4>The high percentage of CD4(+)-T cells (69±3% (mean±SEM/n = 17, by flowcytometry)), measured by flowcytometry and immunohistochemistry, and the presence of non-activated eosinophils found in BE by immunohistochemical staining, were not different from that found in duodenal tissue. Expanded lymphocytes from these tissues had a similar phenotype, characterized by a comparable but low percentage of αE(CD103) positive CD4(+)cells (44±5% in BE, 43±4% in duodenum of BE and 34±7% in duodenum of controls) and a similar percentage of granzyme-B(+)CD8(+) cells(44±5% in BE, 33±6% in duodenum of BE and 36±7% in duodenum of controls). In addition, a similar percentage of α4β7(+) T-lymphocytes (63±5% in BE, 58±5% in duodenum of BE and 62±8% in duodenum of controls) was found. Finally, mRNA expression of the ligand for α4β7, MAdCAM-1, was also similar in BE and duodenal tissue. No evidence for a Th2-response was found as almost no IL-4(+)-T-cells were seen.<h4>Conclusion</h4>The immune cell composition (lymphocytes and eosinophils) and expression of intestinal adhesion molecule MAdCAM-1 is similar in BE and duodenum. This supports the hypothesis that homing of lymphocytes to BE tissue is mainly caused by intestinal homing signals rather than to an active inflammatory response.
format	article
author	Alexandra Lind Peter D Siersema Johannes G Kusters Jan A M Van der Linden Edward F Knol Leo Koenderman
author_facet	Alexandra Lind Peter D Siersema Johannes G Kusters Jan A M Van der Linden Edward F Knol Leo Koenderman
author_sort	Alexandra Lind
title	The immune cell composition in Barrett's metaplastic tissue resembles that in normal duodenal tissue.
title_short	The immune cell composition in Barrett's metaplastic tissue resembles that in normal duodenal tissue.
title_full	The immune cell composition in Barrett's metaplastic tissue resembles that in normal duodenal tissue.
title_fullStr	The immune cell composition in Barrett's metaplastic tissue resembles that in normal duodenal tissue.
title_full_unstemmed	The immune cell composition in Barrett's metaplastic tissue resembles that in normal duodenal tissue.
title_sort	immune cell composition in barrett's metaplastic tissue resembles that in normal duodenal tissue.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/58e78c71269f4c8482975c5772b74c43
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The immune cell composition in Barrett's metaplastic tissue resembles that in normal duodenal tissue.

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