In vitro and in vivo applications of Euphorbia wallichii shoot extract-mediated gold nanospheres

The current study was designed to investigate the potential of Euphorbia wallichii shoot extract for reducting Au3+ and stabilizing gold nanoparticles. UV-visible spectra of gold nanoparticles showed obvious surface plasmon resonance peak at 548 nm. Microscopy (SEM and TEM) showed spherical dimensio...

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Autores principales:	Ullah Rehman, Shah Sumaira, Muhammad Zahir, Shah Sajjad Ali, Faisal Shah, Khattak Umbreen, Haq Tauheed ul, Taj Akbar Muhammad
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	euphorbia wallichii gold nanospheres characterization in vitro in vivo applications Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/58f7cb9c26a0469c90a08b54bd8f5461
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spelling	oai:doaj.org-article:58f7cb9c26a0469c90a08b54bd8f54612021-12-05T14:10:49ZIn vitro and in vivo applications of Euphorbia wallichii shoot extract-mediated gold nanospheres2191-955010.1515/gps-2021-0013https://doaj.org/article/58f7cb9c26a0469c90a08b54bd8f54612021-02-01T00:00:00Zhttps://doi.org/10.1515/gps-2021-0013https://doaj.org/toc/2191-9550The current study was designed to investigate the potential of Euphorbia wallichii shoot extract for reducting Au3+ and stabilizing gold nanoparticles. UV-visible spectra of gold nanoparticles showed obvious surface plasmon resonance peak at 548 nm. Microscopy (SEM and TEM) showed spherical dimensions, and the energy dispersive X-ray spectra displayed the strongest optical absorption peak for gold (Au) at 2.1 keV. Dynamic light scattering spectra represent polydispersed mixture with particulate diameter of 2.5–103.2 nm. The IR spectra confirm the potential functional groups of shoot extract responsible for the reduction of Au3+ to gold nanoparticles which exhibit tremendous antibacterial potential of 76.31%, 68.47%, 79.85%, 48.10%, and 65.53% against Escherichia coli, Staphylococcus aureus, Bacillus pumilus, Pseudomonas aeruginosa, and Klebsiella pneumoniae, respectively. Gold nanoparticles showed markedly elevated fungicidal potency compared to the shoot extract alone against the tested fungal strains. IC50 for 2,2-diphenyl-1-picrylhydrazyl scavenging was 31.52, 18.29, and 15.32 µg/mL at 30, 60, and 90 min of reaction time, respectively. Both shoot extract and nanoparticles revealed 71% mortality at 100 µg/mL, with LD90 values of 310.56 µg/mL. Experimental mice acquired dose-dependent analgesia of 54.21%, 82.60%, and 86.53% when treated with gold nanoparticles at 50, 100, and 200 mg/kg bw. Inhibition of gastrointestinal muscular contraction was 21.16%, 30.49%, and 40.19% in mice feed with 50, 100, and 200 mg/kg bw, respectively.Ullah RehmanShah SumairaMuhammad ZahirShah Sajjad AliFaisal ShahKhattak UmbreenHaq Tauheed ulTaj Akbar MuhammadDe Gruyterarticleeuphorbia wallichiigold nanospherescharacterizationin vitroin vivo applicationsChemistryQD1-999ENGreen Processing and Synthesis, Vol 10, Iss 1, Pp 101-111 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	euphorbia wallichii gold nanospheres characterization in vitro in vivo applications Chemistry QD1-999
spellingShingle	euphorbia wallichii gold nanospheres characterization in vitro in vivo applications Chemistry QD1-999 Ullah Rehman Shah Sumaira Muhammad Zahir Shah Sajjad Ali Faisal Shah Khattak Umbreen Haq Tauheed ul Taj Akbar Muhammad In vitro and in vivo applications of Euphorbia wallichii shoot extract-mediated gold nanospheres
description	The current study was designed to investigate the potential of Euphorbia wallichii shoot extract for reducting Au3+ and stabilizing gold nanoparticles. UV-visible spectra of gold nanoparticles showed obvious surface plasmon resonance peak at 548 nm. Microscopy (SEM and TEM) showed spherical dimensions, and the energy dispersive X-ray spectra displayed the strongest optical absorption peak for gold (Au) at 2.1 keV. Dynamic light scattering spectra represent polydispersed mixture with particulate diameter of 2.5–103.2 nm. The IR spectra confirm the potential functional groups of shoot extract responsible for the reduction of Au3+ to gold nanoparticles which exhibit tremendous antibacterial potential of 76.31%, 68.47%, 79.85%, 48.10%, and 65.53% against Escherichia coli, Staphylococcus aureus, Bacillus pumilus, Pseudomonas aeruginosa, and Klebsiella pneumoniae, respectively. Gold nanoparticles showed markedly elevated fungicidal potency compared to the shoot extract alone against the tested fungal strains. IC50 for 2,2-diphenyl-1-picrylhydrazyl scavenging was 31.52, 18.29, and 15.32 µg/mL at 30, 60, and 90 min of reaction time, respectively. Both shoot extract and nanoparticles revealed 71% mortality at 100 µg/mL, with LD90 values of 310.56 µg/mL. Experimental mice acquired dose-dependent analgesia of 54.21%, 82.60%, and 86.53% when treated with gold nanoparticles at 50, 100, and 200 mg/kg bw. Inhibition of gastrointestinal muscular contraction was 21.16%, 30.49%, and 40.19% in mice feed with 50, 100, and 200 mg/kg bw, respectively.
format	article
author	Ullah Rehman Shah Sumaira Muhammad Zahir Shah Sajjad Ali Faisal Shah Khattak Umbreen Haq Tauheed ul Taj Akbar Muhammad
author_facet	Ullah Rehman Shah Sumaira Muhammad Zahir Shah Sajjad Ali Faisal Shah Khattak Umbreen Haq Tauheed ul Taj Akbar Muhammad
author_sort	Ullah Rehman
title	In vitro and in vivo applications of Euphorbia wallichii shoot extract-mediated gold nanospheres
title_short	In vitro and in vivo applications of Euphorbia wallichii shoot extract-mediated gold nanospheres
title_full	In vitro and in vivo applications of Euphorbia wallichii shoot extract-mediated gold nanospheres
title_fullStr	In vitro and in vivo applications of Euphorbia wallichii shoot extract-mediated gold nanospheres
title_full_unstemmed	In vitro and in vivo applications of Euphorbia wallichii shoot extract-mediated gold nanospheres
title_sort	in vitro and in vivo applications of euphorbia wallichii shoot extract-mediated gold nanospheres
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/58f7cb9c26a0469c90a08b54bd8f5461
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In vitro and in vivo applications of Euphorbia wallichii shoot extract-mediated gold nanospheres

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