Preparation and characterization of Tamoxifen citrate loaded nanoparticles for breast cancer therapy

Ruma Maji, Niladri Shekhar Dey, Bhabani Sankar Satapathy, Biswajit Mukherjee, Subhasish MondalDepartment of Pharmaceutical Technology, Jadavpur University, Kolkata (Calcutta), IndiaBackground: Four formulations of Tamoxifen citrate loaded polylactide-co-glycolide (PLGA) based nanoparticles (TNPs) w...

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Autores principales:	Maji R, Dey NS, Satapathy BS, Mukherjee B, Mondal S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/58fb07e6f91144ddb6b32e1b9bafbb59
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spelling	oai:doaj.org-article:58fb07e6f91144ddb6b32e1b9bafbb592021-12-02T05:27:26ZPreparation and characterization of Tamoxifen citrate loaded nanoparticles for breast cancer therapy1178-2013https://doaj.org/article/58fb07e6f91144ddb6b32e1b9bafbb592014-06-01T00:00:00Zhttp://www.dovepress.com/preparation-and-characterization-of-tamoxifen-citrate-loaded-nanoparti-a17359https://doaj.org/toc/1178-2013 Ruma Maji, Niladri Shekhar Dey, Bhabani Sankar Satapathy, Biswajit Mukherjee, Subhasish MondalDepartment of Pharmaceutical Technology, Jadavpur University, Kolkata (Calcutta), IndiaBackground: Four formulations of Tamoxifen citrate loaded polylactide-co-glycolide (PLGA) based nanoparticles (TNPs) were developed and characterized. Their internalization by Michigan Cancer Foundation-7 (MCF-7) breast cancer cells was also investigated.Methods: Nanoparticles were prepared by a multiple emulsion solvent evaporation method. Then the following studies were carried out: drug-excipients interaction using Fourier transform infrared spectroscopy (FTIR), surface morphology by field emission scanning electron microscopy (FESEM), zeta potential and size distribution using a Zetasizer Nano ZS90 and particle size analyzer, and in vitro drug release. In vitro cellular uptake of nanoparticles was assessed by confocal microscopy and their cell viability (%) was studied.Results: No chemical interaction was observed between the drug and the selected excipients. TNPs had a smooth surface, and a nanosize range (250–380 nm) with a negative surface charge. Drug loadings of the prepared particles were 1.5%±0.02% weight/weight (w/w), 2.68%±0.5% w/w, 4.09%±0.2% w/w, 27.16%±2.08% w/w for NP1–NP4, respectively. A sustained drug release pattern from the nanoparticles was observed for the entire period of study, ie, up to 60 days. Further, nanoparticles were internalized well by the MCF-7 breast cancer cells on a concentration dependent manner and were present in the cytoplasm. The nucleus was free from nanoparticle entry. Drug loaded nanoparticles were found to be more cytotoxic than the free drug.Conclusion: TNPs (NP4) showed the highest drug loading, released the drug in a sustained manner for a prolonged period of time and were taken up well by the MCF-7 breast cancer cell line in vitro. Thus the formulation may be suitable for breast cancer treatment due to the good permeation of the formulation into the breast cancer cells.Keywords: polylactide-co-glycolide nanoparticle, PLGA, breast cancer, multiple emulsionMaji RDey NSSatapathy BSMukherjee BMondal SDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 3107-3118 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Maji R Dey NS Satapathy BS Mukherjee B Mondal S Preparation and characterization of Tamoxifen citrate loaded nanoparticles for breast cancer therapy
description	Ruma Maji, Niladri Shekhar Dey, Bhabani Sankar Satapathy, Biswajit Mukherjee, Subhasish MondalDepartment of Pharmaceutical Technology, Jadavpur University, Kolkata (Calcutta), IndiaBackground: Four formulations of Tamoxifen citrate loaded polylactide-co-glycolide (PLGA) based nanoparticles (TNPs) were developed and characterized. Their internalization by Michigan Cancer Foundation-7 (MCF-7) breast cancer cells was also investigated.Methods: Nanoparticles were prepared by a multiple emulsion solvent evaporation method. Then the following studies were carried out: drug-excipients interaction using Fourier transform infrared spectroscopy (FTIR), surface morphology by field emission scanning electron microscopy (FESEM), zeta potential and size distribution using a Zetasizer Nano ZS90 and particle size analyzer, and in vitro drug release. In vitro cellular uptake of nanoparticles was assessed by confocal microscopy and their cell viability (%) was studied.Results: No chemical interaction was observed between the drug and the selected excipients. TNPs had a smooth surface, and a nanosize range (250–380 nm) with a negative surface charge. Drug loadings of the prepared particles were 1.5%±0.02% weight/weight (w/w), 2.68%±0.5% w/w, 4.09%±0.2% w/w, 27.16%±2.08% w/w for NP1–NP4, respectively. A sustained drug release pattern from the nanoparticles was observed for the entire period of study, ie, up to 60 days. Further, nanoparticles were internalized well by the MCF-7 breast cancer cells on a concentration dependent manner and were present in the cytoplasm. The nucleus was free from nanoparticle entry. Drug loaded nanoparticles were found to be more cytotoxic than the free drug.Conclusion: TNPs (NP4) showed the highest drug loading, released the drug in a sustained manner for a prolonged period of time and were taken up well by the MCF-7 breast cancer cell line in vitro. Thus the formulation may be suitable for breast cancer treatment due to the good permeation of the formulation into the breast cancer cells.Keywords: polylactide-co-glycolide nanoparticle, PLGA, breast cancer, multiple emulsion
format	article
author	Maji R Dey NS Satapathy BS Mukherjee B Mondal S
author_facet	Maji R Dey NS Satapathy BS Mukherjee B Mondal S
author_sort	Maji R
title	Preparation and characterization of Tamoxifen citrate loaded nanoparticles for breast cancer therapy
title_short	Preparation and characterization of Tamoxifen citrate loaded nanoparticles for breast cancer therapy
title_full	Preparation and characterization of Tamoxifen citrate loaded nanoparticles for breast cancer therapy
title_fullStr	Preparation and characterization of Tamoxifen citrate loaded nanoparticles for breast cancer therapy
title_full_unstemmed	Preparation and characterization of Tamoxifen citrate loaded nanoparticles for breast cancer therapy
title_sort	preparation and characterization of tamoxifen citrate loaded nanoparticles for breast cancer therapy
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/58fb07e6f91144ddb6b32e1b9bafbb59
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Preparation and characterization of Tamoxifen citrate loaded nanoparticles for breast cancer therapy

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