Short Communication: First record of Hirschmanniella mucronata (Nematoda: Pratylenchidae) in Yogyakarta, Indonesia

Abstract. Indarti S, Soffan A, Andrasmara MMF. 2020. Short Communication: First record of Hirschmanniella mucronata (Nematoda: Pratylenchidae) in Yogyakarta, Indonesia. Biodiversitas 21: 2068-2073. Hirschmanniella spp. is one of the worldwide plant-parasitic nematodes affecting major losses in rice...

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Autores principales:	Siwi Indarti, Alan Soffan, MUHAMMAD MAULANA FARDANI ANDRASMARA
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	hirschmanniella mucronata, h. oryzae, molecular identification, yogyakarta Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/58fd4a100b074e1e9c32b7403ca419cf
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spelling	oai:doaj.org-article:58fd4a100b074e1e9c32b7403ca419cf2021-11-22T00:35:33ZShort Communication: First record of Hirschmanniella mucronata (Nematoda: Pratylenchidae) in Yogyakarta, Indonesia1412-033X2085-472210.13057/biodiv/d210533https://doaj.org/article/58fd4a100b074e1e9c32b7403ca419cf2020-04-01T00:00:00Zhttps://smujo.id/biodiv/article/view/4567https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Indarti S, Soffan A, Andrasmara MMF. 2020. Short Communication: First record of Hirschmanniella mucronata (Nematoda: Pratylenchidae) in Yogyakarta, Indonesia. Biodiversitas 21: 2068-2073. Hirschmanniella spp. is one of the worldwide plant-parasitic nematodes affecting major losses in rice production and impact up to 25% yield losses especially on irrigated rice. Infection of Hirschmanniella spp. on the root system leading to the typical symptom of red color in the rice rooting system. In order to identify the species variation of Hirschmanniella spp. from the collected samples in Yogyakarta Province of Indonesia, a molecular-based identification using Polymerase Chain Reaction (PCR) method was conducted, complemented by morphological identification technique. PCR based identification was carried out by amplifying the area of ??28S rRNA using universal nematode primer (D2A / D3B) which resulting about 766 bp of amplicon. Blastx analysis from Genbank showed that Cangkringan sample confirmed to be H. mucronata species, while other samples from Banguntapan and Imogiri were H. oryzae species. The Cangkringan samples of H. mucronata have 97.5 % similarities with Belgium sample, forming separate clades with other samples. While both Banguntapan and Imogiri samples have the 99 % similarity with H. oryzae and were located in the same clade, but separated from Cangkringan sample. Morphological identification confirmed both species were distinctly based on the unique characters of the tail tips. H. mucronata therefor is the first report nematode species in Indonesian rice field. Precaution should be designed to prevent the potential distribution of H. mucronata to other areas.Siwi IndartiAlan SoffanMUHAMMAD MAULANA FARDANI ANDRASMARAMBI & UNS Soloarticlehirschmanniella mucronata, h. oryzae, molecular identification, yogyakartaBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 5 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	hirschmanniella mucronata, h. oryzae, molecular identification, yogyakarta Biology (General) QH301-705.5
spellingShingle	hirschmanniella mucronata, h. oryzae, molecular identification, yogyakarta Biology (General) QH301-705.5 Siwi Indarti Alan Soffan MUHAMMAD MAULANA FARDANI ANDRASMARA Short Communication: First record of Hirschmanniella mucronata (Nematoda: Pratylenchidae) in Yogyakarta, Indonesia
description	Abstract. Indarti S, Soffan A, Andrasmara MMF. 2020. Short Communication: First record of Hirschmanniella mucronata (Nematoda: Pratylenchidae) in Yogyakarta, Indonesia. Biodiversitas 21: 2068-2073. Hirschmanniella spp. is one of the worldwide plant-parasitic nematodes affecting major losses in rice production and impact up to 25% yield losses especially on irrigated rice. Infection of Hirschmanniella spp. on the root system leading to the typical symptom of red color in the rice rooting system. In order to identify the species variation of Hirschmanniella spp. from the collected samples in Yogyakarta Province of Indonesia, a molecular-based identification using Polymerase Chain Reaction (PCR) method was conducted, complemented by morphological identification technique. PCR based identification was carried out by amplifying the area of ??28S rRNA using universal nematode primer (D2A / D3B) which resulting about 766 bp of amplicon. Blastx analysis from Genbank showed that Cangkringan sample confirmed to be H. mucronata species, while other samples from Banguntapan and Imogiri were H. oryzae species. The Cangkringan samples of H. mucronata have 97.5 % similarities with Belgium sample, forming separate clades with other samples. While both Banguntapan and Imogiri samples have the 99 % similarity with H. oryzae and were located in the same clade, but separated from Cangkringan sample. Morphological identification confirmed both species were distinctly based on the unique characters of the tail tips. H. mucronata therefor is the first report nematode species in Indonesian rice field. Precaution should be designed to prevent the potential distribution of H. mucronata to other areas.
format	article
author	Siwi Indarti Alan Soffan MUHAMMAD MAULANA FARDANI ANDRASMARA
author_facet	Siwi Indarti Alan Soffan MUHAMMAD MAULANA FARDANI ANDRASMARA
author_sort	Siwi Indarti
title	Short Communication: First record of Hirschmanniella mucronata (Nematoda: Pratylenchidae) in Yogyakarta, Indonesia
title_short	Short Communication: First record of Hirschmanniella mucronata (Nematoda: Pratylenchidae) in Yogyakarta, Indonesia
title_full	Short Communication: First record of Hirschmanniella mucronata (Nematoda: Pratylenchidae) in Yogyakarta, Indonesia
title_fullStr	Short Communication: First record of Hirschmanniella mucronata (Nematoda: Pratylenchidae) in Yogyakarta, Indonesia
title_full_unstemmed	Short Communication: First record of Hirschmanniella mucronata (Nematoda: Pratylenchidae) in Yogyakarta, Indonesia
title_sort	short communication: first record of hirschmanniella mucronata (nematoda: pratylenchidae) in yogyakarta, indonesia
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/58fd4a100b074e1e9c32b7403ca419cf
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Short Communication: First record of Hirschmanniella mucronata (Nematoda: Pratylenchidae) in Yogyakarta, Indonesia

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