Synergistic gene editing in human iPS cells via cell cycle and DNA repair modulation

Precision editing with CRISPR-Cas9 often suffers from poor efficiency. Here the authors identify culture conditions and small molecules that synergize to promote homology-directed repair (HDR) in induced pluripotent stem (iPS) cells.

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Detalles Bibliográficos
Autores principales:	Thomas L. Maurissen, Knut Woltjen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/58ffda05e1954580a8f67c710fef88ab
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spelling	oai:doaj.org-article:58ffda05e1954580a8f67c710fef88ab2021-12-02T17:52:29ZSynergistic gene editing in human iPS cells via cell cycle and DNA repair modulation10.1038/s41467-020-16643-52041-1723https://doaj.org/article/58ffda05e1954580a8f67c710fef88ab2020-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-16643-5https://doaj.org/toc/2041-1723Precision editing with CRISPR-Cas9 often suffers from poor efficiency. Here the authors identify culture conditions and small molecules that synergize to promote homology-directed repair (HDR) in induced pluripotent stem (iPS) cells.Thomas L. MaurissenKnut WoltjenNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-14 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Thomas L. Maurissen Knut Woltjen Synergistic gene editing in human iPS cells via cell cycle and DNA repair modulation
description	Precision editing with CRISPR-Cas9 often suffers from poor efficiency. Here the authors identify culture conditions and small molecules that synergize to promote homology-directed repair (HDR) in induced pluripotent stem (iPS) cells.
format	article
author	Thomas L. Maurissen Knut Woltjen
author_facet	Thomas L. Maurissen Knut Woltjen
author_sort	Thomas L. Maurissen
title	Synergistic gene editing in human iPS cells via cell cycle and DNA repair modulation
title_short	Synergistic gene editing in human iPS cells via cell cycle and DNA repair modulation
title_full	Synergistic gene editing in human iPS cells via cell cycle and DNA repair modulation
title_fullStr	Synergistic gene editing in human iPS cells via cell cycle and DNA repair modulation
title_full_unstemmed	Synergistic gene editing in human iPS cells via cell cycle and DNA repair modulation
title_sort	synergistic gene editing in human ips cells via cell cycle and dna repair modulation
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/58ffda05e1954580a8f67c710fef88ab
work_keys_str_mv	AT thomaslmaurissen synergisticgeneeditinginhumanipscellsviacellcycleanddnarepairmodulation AT knutwoltjen synergisticgeneeditinginhumanipscellsviacellcycleanddnarepairmodulation
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Synergistic gene editing in human iPS cells via cell cycle and DNA repair modulation

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