SpineRacks and SpinalJ for efficient analysis of neurons in a 3D reference atlas of the mouse spinal cord

Summary: Spatial analysis of spinal neurons is currently limited by a lack of tools for efficient preparation and imaging of the whole spinal cord (SC) and the absence of a 3D reference atlas. Here, we describe protocols for efficient sectioning of whole SC using SpineRacks and subsequent image regi...

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Autores principales:	Felix Fiederling, Luke A. Hammond, David Ng, Carol Mason, Jane Dodd
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Developmental biology Microscopy Model Organisms Neuroscience Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/5904038e0ed54a2085d0d0cf1d09614e
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spelling	oai:doaj.org-article:5904038e0ed54a2085d0d0cf1d09614e2021-11-18T04:51:38ZSpineRacks and SpinalJ for efficient analysis of neurons in a 3D reference atlas of the mouse spinal cord2666-166710.1016/j.xpro.2021.100897https://doaj.org/article/5904038e0ed54a2085d0d0cf1d09614e2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666166721006031https://doaj.org/toc/2666-1667Summary: Spatial analysis of spinal neurons is currently limited by a lack of tools for efficient preparation and imaging of the whole spinal cord (SC) and the absence of a 3D reference atlas. Here, we describe protocols for efficient sectioning of whole SC using SpineRacks and subsequent image registration, atlas mapping, and 3D analysis of cells and projections, using SpinalJ. Together, these tools enable high-throughput analyses of adult mouse SC and direct comparison of spatial information of neurons between animals and studies.For complete details on the use and execution of this protocol, please refer to Fiederling et al. (2021).Felix FiederlingLuke A. HammondDavid NgCarol MasonJane DoddElsevierarticleDevelopmental biologyMicroscopyModel OrganismsNeuroscienceScience (General)Q1-390ENSTAR Protocols, Vol 2, Iss 4, Pp 100897- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Developmental biology Microscopy Model Organisms Neuroscience Science (General) Q1-390
spellingShingle	Developmental biology Microscopy Model Organisms Neuroscience Science (General) Q1-390 Felix Fiederling Luke A. Hammond David Ng Carol Mason Jane Dodd SpineRacks and SpinalJ for efficient analysis of neurons in a 3D reference atlas of the mouse spinal cord
description	Summary: Spatial analysis of spinal neurons is currently limited by a lack of tools for efficient preparation and imaging of the whole spinal cord (SC) and the absence of a 3D reference atlas. Here, we describe protocols for efficient sectioning of whole SC using SpineRacks and subsequent image registration, atlas mapping, and 3D analysis of cells and projections, using SpinalJ. Together, these tools enable high-throughput analyses of adult mouse SC and direct comparison of spatial information of neurons between animals and studies.For complete details on the use and execution of this protocol, please refer to Fiederling et al. (2021).
format	article
author	Felix Fiederling Luke A. Hammond David Ng Carol Mason Jane Dodd
author_facet	Felix Fiederling Luke A. Hammond David Ng Carol Mason Jane Dodd
author_sort	Felix Fiederling
title	SpineRacks and SpinalJ for efficient analysis of neurons in a 3D reference atlas of the mouse spinal cord
title_short	SpineRacks and SpinalJ for efficient analysis of neurons in a 3D reference atlas of the mouse spinal cord
title_full	SpineRacks and SpinalJ for efficient analysis of neurons in a 3D reference atlas of the mouse spinal cord
title_fullStr	SpineRacks and SpinalJ for efficient analysis of neurons in a 3D reference atlas of the mouse spinal cord
title_full_unstemmed	SpineRacks and SpinalJ for efficient analysis of neurons in a 3D reference atlas of the mouse spinal cord
title_sort	spineracks and spinalj for efficient analysis of neurons in a 3d reference atlas of the mouse spinal cord
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/5904038e0ed54a2085d0d0cf1d09614e
work_keys_str_mv	AT felixfiederling spineracksandspinaljforefficientanalysisofneuronsina3dreferenceatlasofthemousespinalcord AT lukeahammond spineracksandspinaljforefficientanalysisofneuronsina3dreferenceatlasofthemousespinalcord AT davidng spineracksandspinaljforefficientanalysisofneuronsina3dreferenceatlasofthemousespinalcord AT carolmason spineracksandspinaljforefficientanalysisofneuronsina3dreferenceatlasofthemousespinalcord AT janedodd spineracksandspinaljforefficientanalysisofneuronsina3dreferenceatlasofthemousespinalcord
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SpineRacks and SpinalJ for efficient analysis of neurons in a 3D reference atlas of the mouse spinal cord

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