Rapid and accurate peripheral nerve imaging by multipoint Raman spectroscopy

Abstract Raman spectroscopy allows label-free, minimally invasive, and accurate detection of peripheral nerves. However, the conventional Raman imaging technique is time-consuming when measuring a large area of a sample. Establishing a method for rapidly acquiring spatial distribution of a bundle of...

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Autores principales:	Yasuaki Kumamoto, Yoshinori Harada, Hideo Tanaka, Tetsuro Takamatsu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/590a60a2cfa2421dbd2ab003c540b346
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spelling	oai:doaj.org-article:590a60a2cfa2421dbd2ab003c540b3462021-12-02T16:06:16ZRapid and accurate peripheral nerve imaging by multipoint Raman spectroscopy10.1038/s41598-017-00995-y2045-2322https://doaj.org/article/590a60a2cfa2421dbd2ab003c540b3462017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00995-yhttps://doaj.org/toc/2045-2322Abstract Raman spectroscopy allows label-free, minimally invasive, and accurate detection of peripheral nerves. However, the conventional Raman imaging technique is time-consuming when measuring a large area of a sample. Establishing a method for rapidly acquiring spatial distribution of a bundle of peripheral nerve fibers is an essential step for Raman spectroscopy towards application in clinical surgery. Here we present a multipoint Raman spectroscopic technique for rapid peripheral nerve imaging. In only 5 seconds, spectra at 32 points situated on ex vivo rat peripheral nerve bundles and adjoining connective tissues were acquired. Principal component regression and discriminant analysis of spectra revealed that the sensitivity, specificity and accuracy for nerve detection were 85.8%, 96.0%, and 90.8%, respectively. Of 158 peripheral nerves, 152 (96.2%) showed ratio of the number of nerve-positive prediction points to the total measurement points being 0.4 or larger, whereas 119 (99.2%) connective tissues among 120 showed ratio smaller than 0.4. Based on the ratio and a bright-field image of the sample, accurate visualization of peripheral nerves was implemented. The results indicated that the multipoint Raman spectroscopic technique is capable of rapid and accurate peripheral nerve imaging.Yasuaki KumamotoYoshinori HaradaHideo TanakaTetsuro TakamatsuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yasuaki Kumamoto Yoshinori Harada Hideo Tanaka Tetsuro Takamatsu Rapid and accurate peripheral nerve imaging by multipoint Raman spectroscopy
description	Abstract Raman spectroscopy allows label-free, minimally invasive, and accurate detection of peripheral nerves. However, the conventional Raman imaging technique is time-consuming when measuring a large area of a sample. Establishing a method for rapidly acquiring spatial distribution of a bundle of peripheral nerve fibers is an essential step for Raman spectroscopy towards application in clinical surgery. Here we present a multipoint Raman spectroscopic technique for rapid peripheral nerve imaging. In only 5 seconds, spectra at 32 points situated on ex vivo rat peripheral nerve bundles and adjoining connective tissues were acquired. Principal component regression and discriminant analysis of spectra revealed that the sensitivity, specificity and accuracy for nerve detection were 85.8%, 96.0%, and 90.8%, respectively. Of 158 peripheral nerves, 152 (96.2%) showed ratio of the number of nerve-positive prediction points to the total measurement points being 0.4 or larger, whereas 119 (99.2%) connective tissues among 120 showed ratio smaller than 0.4. Based on the ratio and a bright-field image of the sample, accurate visualization of peripheral nerves was implemented. The results indicated that the multipoint Raman spectroscopic technique is capable of rapid and accurate peripheral nerve imaging.
format	article
author	Yasuaki Kumamoto Yoshinori Harada Hideo Tanaka Tetsuro Takamatsu
author_facet	Yasuaki Kumamoto Yoshinori Harada Hideo Tanaka Tetsuro Takamatsu
author_sort	Yasuaki Kumamoto
title	Rapid and accurate peripheral nerve imaging by multipoint Raman spectroscopy
title_short	Rapid and accurate peripheral nerve imaging by multipoint Raman spectroscopy
title_full	Rapid and accurate peripheral nerve imaging by multipoint Raman spectroscopy
title_fullStr	Rapid and accurate peripheral nerve imaging by multipoint Raman spectroscopy
title_full_unstemmed	Rapid and accurate peripheral nerve imaging by multipoint Raman spectroscopy
title_sort	rapid and accurate peripheral nerve imaging by multipoint raman spectroscopy
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/590a60a2cfa2421dbd2ab003c540b346
work_keys_str_mv	AT yasuakikumamoto rapidandaccurateperipheralnerveimagingbymultipointramanspectroscopy AT yoshinoriharada rapidandaccurateperipheralnerveimagingbymultipointramanspectroscopy AT hideotanaka rapidandaccurateperipheralnerveimagingbymultipointramanspectroscopy AT tetsurotakamatsu rapidandaccurateperipheralnerveimagingbymultipointramanspectroscopy
_version_	1718385022900109312

Rapid and accurate peripheral nerve imaging by multipoint Raman spectroscopy

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