Comparison between gold nanoparticles and FITC as the labelling in lateral flow immunoassays for rapid detection of Ralstonia solanacearum
Early prevention and monitoring of Ralstonia solanacearum (Rs) on nightshade is extremely critical for potent prevention and treatment of bacterial wilt disease. We developed two kinds of lateral flow immunochromatographic strip (LFICS) using gold nanoparticles (GNPs) and fluorescein isothiocyanate...
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Taylor & Francis Group
2018
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oai:doaj.org-article:590e28863d9c4cf5b3b8445c802c03622021-11-26T11:19:47ZComparison between gold nanoparticles and FITC as the labelling in lateral flow immunoassays for rapid detection of Ralstonia solanacearum0954-01051465-344310.1080/09540105.2018.1510474https://doaj.org/article/590e28863d9c4cf5b3b8445c802c03622018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2018.1510474https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443Early prevention and monitoring of Ralstonia solanacearum (Rs) on nightshade is extremely critical for potent prevention and treatment of bacterial wilt disease. We developed two kinds of lateral flow immunochromatographic strip (LFICS) using gold nanoparticles (GNPs) and fluorescein isothiocyanate (FITC) for rapid diagnosis of plant bacterial wilt, caused by Ralstonia solanacearum (Rs), which both were based on double antibody sandwich method. Three monoclonal antibodies (McAb) highly specific to Rs were obtained by cell fusion and screening after four times of immunization with the strain GIM 1.76. Under optimized conditions, the limit of detection (LOD) with GNPs and FITC were 104 colony forming units per millilitre (CFU/mL) and 106 CFU/mL respectively, then the range detection correspondingly were 104–109 CFU/mL and 106–109 CFU/mL. On top of that, the developed immunoassay could be used for spot and point-of-care testing Rs on tomato seed, ginger seed, casuarina seed and display no cross-reaction with 43 other phytopathogenic strains that also could infect plants so as to cause plants diseases. These results demonstrated that we sucessfully developed two simple, effective and sensitive strips for monitoring bacterial wilt infection. Meanwhile, this is the first report about LFICS labelled by FITC for rapid detection of Rs, and it is the first time conformed that the sensitive of the LFICS with GNPs is significantly higher than FITC.Lan MaShujuan WangDongpo XuManman XieChengchao DingYachen TianJingxuan QiuXiang WangQingli DongQing LiuTaylor & Francis Grouparticleralstonia solanacearumlateral flow immunochromatographic stripgold nanoparticlesfluorescein isothiocyanateAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 1074-1085 (2018) |
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ralstonia solanacearum lateral flow immunochromatographic strip gold nanoparticles fluorescein isothiocyanate Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 |
| spellingShingle |
ralstonia solanacearum lateral flow immunochromatographic strip gold nanoparticles fluorescein isothiocyanate Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Lan Ma Shujuan Wang Dongpo Xu Manman Xie Chengchao Ding Yachen Tian Jingxuan Qiu Xiang Wang Qingli Dong Qing Liu Comparison between gold nanoparticles and FITC as the labelling in lateral flow immunoassays for rapid detection of Ralstonia solanacearum |
| description |
Early prevention and monitoring of Ralstonia solanacearum (Rs) on nightshade is extremely critical for potent prevention and treatment of bacterial wilt disease. We developed two kinds of lateral flow immunochromatographic strip (LFICS) using gold nanoparticles (GNPs) and fluorescein isothiocyanate (FITC) for rapid diagnosis of plant bacterial wilt, caused by Ralstonia solanacearum (Rs), which both were based on double antibody sandwich method. Three monoclonal antibodies (McAb) highly specific to Rs were obtained by cell fusion and screening after four times of immunization with the strain GIM 1.76. Under optimized conditions, the limit of detection (LOD) with GNPs and FITC were 104 colony forming units per millilitre (CFU/mL) and 106 CFU/mL respectively, then the range detection correspondingly were 104–109 CFU/mL and 106–109 CFU/mL. On top of that, the developed immunoassay could be used for spot and point-of-care testing Rs on tomato seed, ginger seed, casuarina seed and display no cross-reaction with 43 other phytopathogenic strains that also could infect plants so as to cause plants diseases. These results demonstrated that we sucessfully developed two simple, effective and sensitive strips for monitoring bacterial wilt infection. Meanwhile, this is the first report about LFICS labelled by FITC for rapid detection of Rs, and it is the first time conformed that the sensitive of the LFICS with GNPs is significantly higher than FITC. |
| format |
article |
| author |
Lan Ma Shujuan Wang Dongpo Xu Manman Xie Chengchao Ding Yachen Tian Jingxuan Qiu Xiang Wang Qingli Dong Qing Liu |
| author_facet |
Lan Ma Shujuan Wang Dongpo Xu Manman Xie Chengchao Ding Yachen Tian Jingxuan Qiu Xiang Wang Qingli Dong Qing Liu |
| author_sort |
Lan Ma |
| title |
Comparison between gold nanoparticles and FITC as the labelling in lateral flow immunoassays for rapid detection of Ralstonia solanacearum |
| title_short |
Comparison between gold nanoparticles and FITC as the labelling in lateral flow immunoassays for rapid detection of Ralstonia solanacearum |
| title_full |
Comparison between gold nanoparticles and FITC as the labelling in lateral flow immunoassays for rapid detection of Ralstonia solanacearum |
| title_fullStr |
Comparison between gold nanoparticles and FITC as the labelling in lateral flow immunoassays for rapid detection of Ralstonia solanacearum |
| title_full_unstemmed |
Comparison between gold nanoparticles and FITC as the labelling in lateral flow immunoassays for rapid detection of Ralstonia solanacearum |
| title_sort |
comparison between gold nanoparticles and fitc as the labelling in lateral flow immunoassays for rapid detection of ralstonia solanacearum |
| publisher |
Taylor & Francis Group |
| publishDate |
2018 |
| url |
https://doaj.org/article/590e28863d9c4cf5b3b8445c802c0362 |
| work_keys_str_mv |
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| _version_ |
1718409534573117440 |