Knockdown of lysine (K)-specific demethylase 2B KDM2B inhibits glycolysis and induces autophagy in lung squamous cell carcinoma cells by regulating the phosphatidylinositol 3-kinase/AKT/mammalian target of rapamycin pathway

Lung squamous cell carcinoma (LUSC) is a subtype of non-small cell lung cancer with poor prognosis. This study aimed to explore the role of KDM2B in the development of LUSC. The results of this study demonstrated that KDM2B was upregulated in LUSC tissues and cell lines. Knockdown of KDM2B reduced c...

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Autores principales:	Zhonghai Xie, Hongwei Li, Jin Zang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	kdm2b glycolysis autophagy lung squamous cell carcinoma pi3k/akt/mtor pathway Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/590ec5d7227d45da80adf8d93bac7ec8
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spelling	oai:doaj.org-article:590ec5d7227d45da80adf8d93bac7ec82021-11-17T14:21:59ZKnockdown of lysine (K)-specific demethylase 2B KDM2B inhibits glycolysis and induces autophagy in lung squamous cell carcinoma cells by regulating the phosphatidylinositol 3-kinase/AKT/mammalian target of rapamycin pathway2165-59792165-598710.1080/21655979.2021.2005931https://doaj.org/article/590ec5d7227d45da80adf8d93bac7ec82021-11-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.2005931https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Lung squamous cell carcinoma (LUSC) is a subtype of non-small cell lung cancer with poor prognosis. This study aimed to explore the role of KDM2B in the development of LUSC. The results of this study demonstrated that KDM2B was upregulated in LUSC tissues and cell lines. Knockdown of KDM2B reduced cell viability and colony forming ability in SK-MES-1 and NCI-H520 cells. KDM2B inhibition reduced glucose consumption, lactate production, ATP level, and also downregulated the expression of LDHA and GLUT1. KDM2B knockdown decreased the protein expression of LC3-I and p62, and increased LC3-II and Beclin-1. Furthermore, KDM2B silencing inhibited the phosphorylation of AKT, mTOR and P70S6K. KDM2B knockdown led to reduced tumor size in mouse model. In conclusion, KDM2B is upregulated in LUSC tissues and cell lines. KDM2B silencing inhibits glycolysis and promotes autophagy through inactivation of the PI3K/Akt/mTOR signaling pathway.Zhonghai XieHongwei LiJin ZangTaylor & Francis Grouparticlekdm2bglycolysisautophagylung squamous cell carcinomapi3k/akt/mtor pathwayBiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	kdm2b glycolysis autophagy lung squamous cell carcinoma pi3k/akt/mtor pathway Biotechnology TP248.13-248.65
spellingShingle	kdm2b glycolysis autophagy lung squamous cell carcinoma pi3k/akt/mtor pathway Biotechnology TP248.13-248.65 Zhonghai Xie Hongwei Li Jin Zang Knockdown of lysine (K)-specific demethylase 2B KDM2B inhibits glycolysis and induces autophagy in lung squamous cell carcinoma cells by regulating the phosphatidylinositol 3-kinase/AKT/mammalian target of rapamycin pathway
description	Lung squamous cell carcinoma (LUSC) is a subtype of non-small cell lung cancer with poor prognosis. This study aimed to explore the role of KDM2B in the development of LUSC. The results of this study demonstrated that KDM2B was upregulated in LUSC tissues and cell lines. Knockdown of KDM2B reduced cell viability and colony forming ability in SK-MES-1 and NCI-H520 cells. KDM2B inhibition reduced glucose consumption, lactate production, ATP level, and also downregulated the expression of LDHA and GLUT1. KDM2B knockdown decreased the protein expression of LC3-I and p62, and increased LC3-II and Beclin-1. Furthermore, KDM2B silencing inhibited the phosphorylation of AKT, mTOR and P70S6K. KDM2B knockdown led to reduced tumor size in mouse model. In conclusion, KDM2B is upregulated in LUSC tissues and cell lines. KDM2B silencing inhibits glycolysis and promotes autophagy through inactivation of the PI3K/Akt/mTOR signaling pathway.
format	article
author	Zhonghai Xie Hongwei Li Jin Zang
author_facet	Zhonghai Xie Hongwei Li Jin Zang
author_sort	Zhonghai Xie
title	Knockdown of lysine (K)-specific demethylase 2B KDM2B inhibits glycolysis and induces autophagy in lung squamous cell carcinoma cells by regulating the phosphatidylinositol 3-kinase/AKT/mammalian target of rapamycin pathway
title_short	Knockdown of lysine (K)-specific demethylase 2B KDM2B inhibits glycolysis and induces autophagy in lung squamous cell carcinoma cells by regulating the phosphatidylinositol 3-kinase/AKT/mammalian target of rapamycin pathway
title_full	Knockdown of lysine (K)-specific demethylase 2B KDM2B inhibits glycolysis and induces autophagy in lung squamous cell carcinoma cells by regulating the phosphatidylinositol 3-kinase/AKT/mammalian target of rapamycin pathway
title_fullStr	Knockdown of lysine (K)-specific demethylase 2B KDM2B inhibits glycolysis and induces autophagy in lung squamous cell carcinoma cells by regulating the phosphatidylinositol 3-kinase/AKT/mammalian target of rapamycin pathway
title_full_unstemmed	Knockdown of lysine (K)-specific demethylase 2B KDM2B inhibits glycolysis and induces autophagy in lung squamous cell carcinoma cells by regulating the phosphatidylinositol 3-kinase/AKT/mammalian target of rapamycin pathway
title_sort	knockdown of lysine (k)-specific demethylase 2b kdm2b inhibits glycolysis and induces autophagy in lung squamous cell carcinoma cells by regulating the phosphatidylinositol 3-kinase/akt/mammalian target of rapamycin pathway
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/590ec5d7227d45da80adf8d93bac7ec8
work_keys_str_mv	AT zhonghaixie knockdownoflysinekspecificdemethylase2bkdm2binhibitsglycolysisandinducesautophagyinlungsquamouscellcarcinomacellsbyregulatingthephosphatidylinositol3kinaseaktmammaliantargetofrapamycinpathway AT hongweili knockdownoflysinekspecificdemethylase2bkdm2binhibitsglycolysisandinducesautophagyinlungsquamouscellcarcinomacellsbyregulatingthephosphatidylinositol3kinaseaktmammaliantargetofrapamycinpathway AT jinzang knockdownoflysinekspecificdemethylase2bkdm2binhibitsglycolysisandinducesautophagyinlungsquamouscellcarcinomacellsbyregulatingthephosphatidylinositol3kinaseaktmammaliantargetofrapamycinpathway
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Knockdown of lysine (K)-specific demethylase 2B KDM2B inhibits glycolysis and induces autophagy in lung squamous cell carcinoma cells by regulating the phosphatidylinositol 3-kinase/AKT/mammalian target of rapamycin pathway

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