A rapid nanobiosensing platform based on herceptin-conjugated graphene for ultrasensitive detection of circulating tumor cells in early breast cancer
In this paper, we use a simple and cheap approach for the synthesis of herceptin-conjugated graphene biosensor to detect the HER2-positive breast cancer cells. The bifunctional graphene-herceptin nanosheets are prepared from graphite by a simple ultrasonic-mediated technique. The prepared protein-me...
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De Gruyter
2021
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oai:doaj.org-article:5910230ca4d0490d9892898668a5f7a02021-12-05T14:10:57ZA rapid nanobiosensing platform based on herceptin-conjugated graphene for ultrasensitive detection of circulating tumor cells in early breast cancer2191-909710.1515/ntrev-2021-0049https://doaj.org/article/5910230ca4d0490d9892898668a5f7a02021-08-01T00:00:00Zhttps://doi.org/10.1515/ntrev-2021-0049https://doaj.org/toc/2191-9097In this paper, we use a simple and cheap approach for the synthesis of herceptin-conjugated graphene biosensor to detect the HER2-positive breast cancer cells. The bifunctional graphene-herceptin nanosheets are prepared from graphite by a simple ultrasonic-mediated technique. The prepared protein-mediated graphene is fully characterized. The results show the exfoliation of graphene layers in herceptin solution. Moreover, herceptin is effectively conjugated into the surface of graphene nanosheets. The synthesized herceptin-conjugated graphene is applied for breast cancer detection. The linear range of this biosensor is 1–80 cells, which is significant. The biosensor shows an excellent selectivity performance for detection of HER2-positive cancer cells. Likewise, the stability and functionality of the biosensor is about 40 days. Based on the results, this device is a promising candidate for rapid and selective detection of cancer cells.Rahimzadeh ZahraNaghib Seyed MortezaAskari EsfandyarMolaabasi FatemehSadr AliZare YasserAfsharpad MandanaRhee Kyong YopDe Gruyterarticlesingle cellbreast cancerimmunosensorherceptingrapheneTechnologyTChemical technologyTP1-1185Physical and theoretical chemistryQD450-801ENNanotechnology Reviews, Vol 10, Iss 1, Pp 744-753 (2021) |
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DOAJ |
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EN |
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single cell breast cancer immunosensor herceptin graphene Technology T Chemical technology TP1-1185 Physical and theoretical chemistry QD450-801 |
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single cell breast cancer immunosensor herceptin graphene Technology T Chemical technology TP1-1185 Physical and theoretical chemistry QD450-801 Rahimzadeh Zahra Naghib Seyed Morteza Askari Esfandyar Molaabasi Fatemeh Sadr Ali Zare Yasser Afsharpad Mandana Rhee Kyong Yop A rapid nanobiosensing platform based on herceptin-conjugated graphene for ultrasensitive detection of circulating tumor cells in early breast cancer |
| description |
In this paper, we use a simple and cheap approach for the synthesis of herceptin-conjugated graphene biosensor to detect the HER2-positive breast cancer cells. The bifunctional graphene-herceptin nanosheets are prepared from graphite by a simple ultrasonic-mediated technique. The prepared protein-mediated graphene is fully characterized. The results show the exfoliation of graphene layers in herceptin solution. Moreover, herceptin is effectively conjugated into the surface of graphene nanosheets. The synthesized herceptin-conjugated graphene is applied for breast cancer detection. The linear range of this biosensor is 1–80 cells, which is significant. The biosensor shows an excellent selectivity performance for detection of HER2-positive cancer cells. Likewise, the stability and functionality of the biosensor is about 40 days. Based on the results, this device is a promising candidate for rapid and selective detection of cancer cells. |
| format |
article |
| author |
Rahimzadeh Zahra Naghib Seyed Morteza Askari Esfandyar Molaabasi Fatemeh Sadr Ali Zare Yasser Afsharpad Mandana Rhee Kyong Yop |
| author_facet |
Rahimzadeh Zahra Naghib Seyed Morteza Askari Esfandyar Molaabasi Fatemeh Sadr Ali Zare Yasser Afsharpad Mandana Rhee Kyong Yop |
| author_sort |
Rahimzadeh Zahra |
| title |
A rapid nanobiosensing platform based on herceptin-conjugated graphene for ultrasensitive detection of circulating tumor cells in early breast cancer |
| title_short |
A rapid nanobiosensing platform based on herceptin-conjugated graphene for ultrasensitive detection of circulating tumor cells in early breast cancer |
| title_full |
A rapid nanobiosensing platform based on herceptin-conjugated graphene for ultrasensitive detection of circulating tumor cells in early breast cancer |
| title_fullStr |
A rapid nanobiosensing platform based on herceptin-conjugated graphene for ultrasensitive detection of circulating tumor cells in early breast cancer |
| title_full_unstemmed |
A rapid nanobiosensing platform based on herceptin-conjugated graphene for ultrasensitive detection of circulating tumor cells in early breast cancer |
| title_sort |
rapid nanobiosensing platform based on herceptin-conjugated graphene for ultrasensitive detection of circulating tumor cells in early breast cancer |
| publisher |
De Gruyter |
| publishDate |
2021 |
| url |
https://doaj.org/article/5910230ca4d0490d9892898668a5f7a0 |
| work_keys_str_mv |
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