Specifically targeted delivery of protein to phagocytic macrophages

Specifically targeted delivery of protein to phagocytic macrophages

Min Yu, Zeming Chen, Wenjun Guo, Jin Wang, Yupeng Feng, Xiuqi Kong, Zhangyong Hong State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, Tianjin, People’s Republic of China Abstract: Macrophages play important roles in the pathogenesis of vario...

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Autores principales: Yu M, Chen Z, Guo W, Wang J, Feng Y, Kong X, Hong Z
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2015
Materias:
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/5911047da77e4df8923ebad737aaf3a6
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spelling oai:doaj.org-article:5911047da77e4df8923ebad737aaf3a62021-12-02T01:32:34ZSpecifically targeted delivery of protein to phagocytic macrophages1178-2013https://doaj.org/article/5911047da77e4df8923ebad737aaf3a62015-03-01T00:00:00Zhttp://www.dovepress.com/specifically-targeted-delivery-of-protein-tonbspphagocytic-macrophages-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Min Yu, Zeming Chen, Wenjun Guo, Jin Wang, Yupeng Feng, Xiuqi Kong, Zhangyong Hong State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, Tianjin, People’s Republic of China Abstract: Macrophages play important roles in the pathogenesis of various diseases, and are important potential therapeutic targets. Furthermore, macrophages are key antigen-presenting cells and important in vaccine design. In this study, we report on the novel formulation (bovine serum albumin [BSA]-loaded glucan particles [GMP-BSA]) based on β-glucan particles from cell walls of baker’s yeast for the targeted delivery of protein to macrophages. Using this formulation, chitosan, tripolyphosphate, and alginate were used to fabricate colloidal particles with the model protein BSA via electrostatic interactions, which were caged and incorporated BSA very tightly within the β-glucan particle shells. The prepared GMP-BSA exhibited good protein-release behavior and avoided protein leakage. The particles were also highly specific to phagocytic macrophages, such as Raw 264.7 cells, primary bone marrow-derived macrophages, and peritoneal exudate macrophages, whereas the particles were not taken up by nonphagocytic cells, including NIH3T3, AD293, HeLa, and Caco-2. We hypothesize that these tightly encapsulated protein-loaded glucan particles deliver various types of proteins to macrophages with notably high selectivity, and may have broad applications in targeted drug delivery or vaccine design against macrophages. Keywords: macrophage, targeted drug delivery, vaccine, glucan particle, yeast-cell wallYu MChen ZGuo WWang JFeng YKong XHong ZDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 1743-1757 (2015)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Yu M
Chen Z
Guo W
Wang J
Feng Y
Kong X
Hong Z
Specifically targeted delivery of protein to phagocytic macrophages
description Min Yu, Zeming Chen, Wenjun Guo, Jin Wang, Yupeng Feng, Xiuqi Kong, Zhangyong Hong State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, Tianjin, People’s Republic of China Abstract: Macrophages play important roles in the pathogenesis of various diseases, and are important potential therapeutic targets. Furthermore, macrophages are key antigen-presenting cells and important in vaccine design. In this study, we report on the novel formulation (bovine serum albumin [BSA]-loaded glucan particles [GMP-BSA]) based on β-glucan particles from cell walls of baker’s yeast for the targeted delivery of protein to macrophages. Using this formulation, chitosan, tripolyphosphate, and alginate were used to fabricate colloidal particles with the model protein BSA via electrostatic interactions, which were caged and incorporated BSA very tightly within the β-glucan particle shells. The prepared GMP-BSA exhibited good protein-release behavior and avoided protein leakage. The particles were also highly specific to phagocytic macrophages, such as Raw 264.7 cells, primary bone marrow-derived macrophages, and peritoneal exudate macrophages, whereas the particles were not taken up by nonphagocytic cells, including NIH3T3, AD293, HeLa, and Caco-2. We hypothesize that these tightly encapsulated protein-loaded glucan particles deliver various types of proteins to macrophages with notably high selectivity, and may have broad applications in targeted drug delivery or vaccine design against macrophages. Keywords: macrophage, targeted drug delivery, vaccine, glucan particle, yeast-cell wall
format article
author Yu M
Chen Z
Guo W
Wang J
Feng Y
Kong X
Hong Z
author_facet Yu M
Chen Z
Guo W
Wang J
Feng Y
Kong X
Hong Z
author_sort Yu M
title Specifically targeted delivery of protein to phagocytic macrophages
title_short Specifically targeted delivery of protein to phagocytic macrophages
title_full Specifically targeted delivery of protein to phagocytic macrophages
title_fullStr Specifically targeted delivery of protein to phagocytic macrophages
title_full_unstemmed Specifically targeted delivery of protein to phagocytic macrophages
title_sort specifically targeted delivery of protein to phagocytic macrophages
publisher Dove Medical Press
publishDate 2015
url https://doaj.org/article/5911047da77e4df8923ebad737aaf3a6
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