<i>N</i>-Methyl-<span style="font-variant: small-caps">d</span>-aspartic Acid (NMDA) Receptor Is Involved in the Inhibitory Effect of Ketamine on Human Sperm Functions

Ketamine, which used to be widely applied in human and animal medicine as a dissociative anesthetic, has become a popular recreational drug because of its hallucinogenic effect. Our previous study preliminarily proved that ketamine could inhibit human sperm function by affecting intracellular calciu...

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Autores principales:	Ying Chen, Wenqing Xu, Yuan Yuan, Houyang Chen, Shuangyan Zheng, Yuanqiao He, Tao Luo
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	NMDA receptor NMDA ketamine human sperm functions intracellular calcium concentration Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/591a1fab8a6544429f914dcb03dc1f2d
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spelling	oai:doaj.org-article:591a1fab8a6544429f914dcb03dc1f2d2021-11-25T17:55:52Z<i>N</i>-Methyl-<span style="font-variant: small-caps">d</span>-aspartic Acid (NMDA) Receptor Is Involved in the Inhibitory Effect of Ketamine on Human Sperm Functions10.3390/ijms2222123701422-00671661-6596https://doaj.org/article/591a1fab8a6544429f914dcb03dc1f2d2021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12370https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Ketamine, which used to be widely applied in human and animal medicine as a dissociative anesthetic, has become a popular recreational drug because of its hallucinogenic effect. Our previous study preliminarily proved that ketamine could inhibit human sperm function by affecting intracellular calcium concentration ([Ca<sup>2+</sup>]<sub>i</sub>). However, the specific signaling pathway of [Ca<sup>2+</sup>]<sub>i</sub> induced by ketamine in human sperm is still not clear yet. Here, the <i>N</i>-methyl-<span style="font-variant: small-caps;">d</span>-aspartic acid (NMDA) receptor was detected in the tail region of human sperm. Its physiological ligand, NMDA (50 μM), could reverse ketamine’s inhibitory effect on human sperm function, and its antagonist, MK801 (100 μM), could restrain the effect of NMDA. The inhibitory effect caused by 4 mM ketamine or 100 μM MK801 on [Ca<sup>2+</sup>]<sub>i</sub>, which is a central factor in the regulation of human sperm function, could also be recovered by 50 μM NMDA. The results suggest that the NMDA receptor is probably involved in the inhibitory effect of ketamine on human sperm functions.Ying ChenWenqing XuYuan YuanHouyang ChenShuangyan ZhengYuanqiao HeTao LuoMDPI AGarticleNMDA receptorNMDAketaminehuman sperm functionsintracellular calcium concentrationBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12370, p 12370 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	NMDA receptor NMDA ketamine human sperm functions intracellular calcium concentration Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	NMDA receptor NMDA ketamine human sperm functions intracellular calcium concentration Biology (General) QH301-705.5 Chemistry QD1-999 Ying Chen Wenqing Xu Yuan Yuan Houyang Chen Shuangyan Zheng Yuanqiao He Tao Luo <i>N</i>-Methyl-<span style="font-variant: small-caps">d</span>-aspartic Acid (NMDA) Receptor Is Involved in the Inhibitory Effect of Ketamine on Human Sperm Functions
description	Ketamine, which used to be widely applied in human and animal medicine as a dissociative anesthetic, has become a popular recreational drug because of its hallucinogenic effect. Our previous study preliminarily proved that ketamine could inhibit human sperm function by affecting intracellular calcium concentration ([Ca<sup>2+</sup>]<sub>i</sub>). However, the specific signaling pathway of [Ca<sup>2+</sup>]<sub>i</sub> induced by ketamine in human sperm is still not clear yet. Here, the <i>N</i>-methyl-<span style="font-variant: small-caps;">d</span>-aspartic acid (NMDA) receptor was detected in the tail region of human sperm. Its physiological ligand, NMDA (50 μM), could reverse ketamine’s inhibitory effect on human sperm function, and its antagonist, MK801 (100 μM), could restrain the effect of NMDA. The inhibitory effect caused by 4 mM ketamine or 100 μM MK801 on [Ca<sup>2+</sup>]<sub>i</sub>, which is a central factor in the regulation of human sperm function, could also be recovered by 50 μM NMDA. The results suggest that the NMDA receptor is probably involved in the inhibitory effect of ketamine on human sperm functions.
format	article
author	Ying Chen Wenqing Xu Yuan Yuan Houyang Chen Shuangyan Zheng Yuanqiao He Tao Luo
author_facet	Ying Chen Wenqing Xu Yuan Yuan Houyang Chen Shuangyan Zheng Yuanqiao He Tao Luo
author_sort	Ying Chen
title	<i>N</i>-Methyl-<span style="font-variant: small-caps">d</span>-aspartic Acid (NMDA) Receptor Is Involved in the Inhibitory Effect of Ketamine on Human Sperm Functions
title_short	<i>N</i>-Methyl-<span style="font-variant: small-caps">d</span>-aspartic Acid (NMDA) Receptor Is Involved in the Inhibitory Effect of Ketamine on Human Sperm Functions
title_full	<i>N</i>-Methyl-<span style="font-variant: small-caps">d</span>-aspartic Acid (NMDA) Receptor Is Involved in the Inhibitory Effect of Ketamine on Human Sperm Functions
title_fullStr	<i>N</i>-Methyl-<span style="font-variant: small-caps">d</span>-aspartic Acid (NMDA) Receptor Is Involved in the Inhibitory Effect of Ketamine on Human Sperm Functions
title_full_unstemmed	<i>N</i>-Methyl-<span style="font-variant: small-caps">d</span>-aspartic Acid (NMDA) Receptor Is Involved in the Inhibitory Effect of Ketamine on Human Sperm Functions
title_sort	<i>n</i>-methyl-<span style="font-variant: small-caps">d</span>-aspartic acid (nmda) receptor is involved in the inhibitory effect of ketamine on human sperm functions
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/591a1fab8a6544429f914dcb03dc1f2d
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<i>N</i>-Methyl-<span style="font-variant: small-caps">d</span>-aspartic Acid (NMDA) Receptor Is Involved in the Inhibitory Effect of Ketamine on Human Sperm Functions

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