Streamlined ex vivo and in vivo genome editing in mouse embryos using recombinant adeno-associated viruses

Streamlined ex vivo and in vivo genome editing in mouse embryos using recombinant adeno-associated viruses

CRISPR-Cas9 has been widely adopted for genetically manipulating rodents for scientific research. Here the authors transduce mouse embryos with CRISPR-Cas9 components using rAAVs in explant culture or in vivo to produce gene-edited animals.

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Autores principales: Yeonsoo Yoon, Dan Wang, Phillip W. L. Tai, Joy Riley, Guangping Gao, Jaime A. Rivera-Pérez
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2018
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Science
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Acceso en línea:https://doaj.org/article/591a750750ef423ca69ffb9deafcbf61
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