Shotgun proteomics reveals putative polyesterases in the secretome of the rock-inhabiting fungus Knufia chersonesos

Abstract Knufia chersonesos is an ascomycotal representative of black fungi, a morphological group of polyextremotolerant melanotic fungi, whose ability to resort to recalcitrant carbon sources makes it an interesting candidate for degradation purposes. A secretome screening towards polyesterases wa...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Donatella Tesei, Felice Quartinello, Georg M. Guebitz, Doris Ribitsch, Katharina Nöbauer, Ebrahim Razzazi-Fazeli, Katja Sterflinger
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/591ae51d10d34990b5091d3532a87251
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:591ae51d10d34990b5091d3532a87251
record_format	dspace
spelling	oai:doaj.org-article:591ae51d10d34990b5091d3532a872512021-12-02T17:40:46ZShotgun proteomics reveals putative polyesterases in the secretome of the rock-inhabiting fungus Knufia chersonesos10.1038/s41598-020-66256-72045-2322https://doaj.org/article/591ae51d10d34990b5091d3532a872512020-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-66256-7https://doaj.org/toc/2045-2322Abstract Knufia chersonesos is an ascomycotal representative of black fungi, a morphological group of polyextremotolerant melanotic fungi, whose ability to resort to recalcitrant carbon sources makes it an interesting candidate for degradation purposes. A secretome screening towards polyesterases was carried out for the fungus and its non-melanized mutant, grown in presence of the synthetic copolyester Polybutylene adipate terephthalate (PBAT) as additional or sole carbon source, and resulted in the identification of 37 esterolytic and lipolytic enzymes across the established cultivation conditions. Quantitative proteomics allowed to unveil 9 proteins being constitutively expressed at all conditions and 7 which were instead detected as up-regulated by PBAT exposure. Protein functional analysis and structure prediction indicated similarity of these enzymes to microbial polyesterases of known biotechnological use such as MHETase from Ideonella sakaiensis and CalA from Candida antarctica. For both strains, PBAT hydrolysis was recorded at all cultivation conditions and primarily the corresponding monomers were released, which suggests degradation to the polymer’s smallest building block. The work presented here aims to demonstrate how investigations of the secretome can provide new insights into the eco-physiology of polymer degrading fungi and ultimately aid the identification of novel enzymes with potential application in polymer processing, recycling and degradation.Donatella TeseiFelice QuartinelloGeorg M. GuebitzDoris RibitschKatharina NöbauerEbrahim Razzazi-FazeliKatja SterflingerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-15 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Donatella Tesei Felice Quartinello Georg M. Guebitz Doris Ribitsch Katharina Nöbauer Ebrahim Razzazi-Fazeli Katja Sterflinger Shotgun proteomics reveals putative polyesterases in the secretome of the rock-inhabiting fungus Knufia chersonesos
description	Abstract Knufia chersonesos is an ascomycotal representative of black fungi, a morphological group of polyextremotolerant melanotic fungi, whose ability to resort to recalcitrant carbon sources makes it an interesting candidate for degradation purposes. A secretome screening towards polyesterases was carried out for the fungus and its non-melanized mutant, grown in presence of the synthetic copolyester Polybutylene adipate terephthalate (PBAT) as additional or sole carbon source, and resulted in the identification of 37 esterolytic and lipolytic enzymes across the established cultivation conditions. Quantitative proteomics allowed to unveil 9 proteins being constitutively expressed at all conditions and 7 which were instead detected as up-regulated by PBAT exposure. Protein functional analysis and structure prediction indicated similarity of these enzymes to microbial polyesterases of known biotechnological use such as MHETase from Ideonella sakaiensis and CalA from Candida antarctica. For both strains, PBAT hydrolysis was recorded at all cultivation conditions and primarily the corresponding monomers were released, which suggests degradation to the polymer’s smallest building block. The work presented here aims to demonstrate how investigations of the secretome can provide new insights into the eco-physiology of polymer degrading fungi and ultimately aid the identification of novel enzymes with potential application in polymer processing, recycling and degradation.
format	article
author	Donatella Tesei Felice Quartinello Georg M. Guebitz Doris Ribitsch Katharina Nöbauer Ebrahim Razzazi-Fazeli Katja Sterflinger
author_facet	Donatella Tesei Felice Quartinello Georg M. Guebitz Doris Ribitsch Katharina Nöbauer Ebrahim Razzazi-Fazeli Katja Sterflinger
author_sort	Donatella Tesei
title	Shotgun proteomics reveals putative polyesterases in the secretome of the rock-inhabiting fungus Knufia chersonesos
title_short	Shotgun proteomics reveals putative polyesterases in the secretome of the rock-inhabiting fungus Knufia chersonesos
title_full	Shotgun proteomics reveals putative polyesterases in the secretome of the rock-inhabiting fungus Knufia chersonesos
title_fullStr	Shotgun proteomics reveals putative polyesterases in the secretome of the rock-inhabiting fungus Knufia chersonesos
title_full_unstemmed	Shotgun proteomics reveals putative polyesterases in the secretome of the rock-inhabiting fungus Knufia chersonesos
title_sort	shotgun proteomics reveals putative polyesterases in the secretome of the rock-inhabiting fungus knufia chersonesos
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/591ae51d10d34990b5091d3532a87251
work_keys_str_mv	AT donatellatesei shotgunproteomicsrevealsputativepolyesterasesinthesecretomeoftherockinhabitingfungusknufiachersonesos AT felicequartinello shotgunproteomicsrevealsputativepolyesterasesinthesecretomeoftherockinhabitingfungusknufiachersonesos AT georgmguebitz shotgunproteomicsrevealsputativepolyesterasesinthesecretomeoftherockinhabitingfungusknufiachersonesos AT dorisribitsch shotgunproteomicsrevealsputativepolyesterasesinthesecretomeoftherockinhabitingfungusknufiachersonesos AT katharinanobauer shotgunproteomicsrevealsputativepolyesterasesinthesecretomeoftherockinhabitingfungusknufiachersonesos AT ebrahimrazzazifazeli shotgunproteomicsrevealsputativepolyesterasesinthesecretomeoftherockinhabitingfungusknufiachersonesos AT katjasterflinger shotgunproteomicsrevealsputativepolyesterasesinthesecretomeoftherockinhabitingfungusknufiachersonesos
_version_	1718379764595556352

Shotgun proteomics reveals putative polyesterases in the secretome of the rock-inhabiting fungus Knufia chersonesos

Ejemplares similares