Nitrogen deficiency results in changes to cell wall composition of sorghum seedlings

Abstract Sorghum [Sorghum bicolor (L.) Moench] has been gaining attention as a feedstock for biomass energy production. While it is obvious that nitrogen (N) supply significantly affects sorghum growth and biomass accumulation, our knowledge is still limited regarding the effect of N on the biomass...

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Autores principales:	Reza Ramdan Rivai, Takuji Miyamoto, Tatsuya Awano, Rie Takada, Yuki Tobimatsu, Toshiaki Umezawa, Masaru Kobayashi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/59220d95da344d25b22927cc1cb7e9d0
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spelling	oai:doaj.org-article:59220d95da344d25b22927cc1cb7e9d02021-12-05T12:13:40ZNitrogen deficiency results in changes to cell wall composition of sorghum seedlings10.1038/s41598-021-02570-y2045-2322https://doaj.org/article/59220d95da344d25b22927cc1cb7e9d02021-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-02570-yhttps://doaj.org/toc/2045-2322Abstract Sorghum [Sorghum bicolor (L.) Moench] has been gaining attention as a feedstock for biomass energy production. While it is obvious that nitrogen (N) supply significantly affects sorghum growth and biomass accumulation, our knowledge is still limited regarding the effect of N on the biomass quality of sorghum, such as the contents and structures of lignin and other cell wall components. Therefore, in this study, we investigated the effects of N supply on the structure and composition of sorghum cell walls. The cell walls of hydroponically cultured sorghum seedlings grown under sufficient or deficient N conditions were analyzed using chemical, two-dimensional nuclear magnetic resonance, gene expression, and immunohistochemical methods. We found that the level of N supply considerably affected the cell wall structure and composition of sorghum seedlings. Limitation of N led to a decrease in the syringyl/guaiacyl lignin unit ratio and an increase in the amount and alteration of tissue distribution of several hemicelluloses, including mixed linkage (1 → 3), (1 → 4)-β-d-glucan, and arabinoxylan. At least some of these cell wall alterations could be associated with changes in gene expression. Nitrogen status is thus one of the factors affecting the cell wall properties of sorghum seedlings.Reza Ramdan RivaiTakuji MiyamotoTatsuya AwanoRie TakadaYuki TobimatsuToshiaki UmezawaMasaru KobayashiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Reza Ramdan Rivai Takuji Miyamoto Tatsuya Awano Rie Takada Yuki Tobimatsu Toshiaki Umezawa Masaru Kobayashi Nitrogen deficiency results in changes to cell wall composition of sorghum seedlings
description	Abstract Sorghum [Sorghum bicolor (L.) Moench] has been gaining attention as a feedstock for biomass energy production. While it is obvious that nitrogen (N) supply significantly affects sorghum growth and biomass accumulation, our knowledge is still limited regarding the effect of N on the biomass quality of sorghum, such as the contents and structures of lignin and other cell wall components. Therefore, in this study, we investigated the effects of N supply on the structure and composition of sorghum cell walls. The cell walls of hydroponically cultured sorghum seedlings grown under sufficient or deficient N conditions were analyzed using chemical, two-dimensional nuclear magnetic resonance, gene expression, and immunohistochemical methods. We found that the level of N supply considerably affected the cell wall structure and composition of sorghum seedlings. Limitation of N led to a decrease in the syringyl/guaiacyl lignin unit ratio and an increase in the amount and alteration of tissue distribution of several hemicelluloses, including mixed linkage (1 → 3), (1 → 4)-β-d-glucan, and arabinoxylan. At least some of these cell wall alterations could be associated with changes in gene expression. Nitrogen status is thus one of the factors affecting the cell wall properties of sorghum seedlings.
format	article
author	Reza Ramdan Rivai Takuji Miyamoto Tatsuya Awano Rie Takada Yuki Tobimatsu Toshiaki Umezawa Masaru Kobayashi
author_facet	Reza Ramdan Rivai Takuji Miyamoto Tatsuya Awano Rie Takada Yuki Tobimatsu Toshiaki Umezawa Masaru Kobayashi
author_sort	Reza Ramdan Rivai
title	Nitrogen deficiency results in changes to cell wall composition of sorghum seedlings
title_short	Nitrogen deficiency results in changes to cell wall composition of sorghum seedlings
title_full	Nitrogen deficiency results in changes to cell wall composition of sorghum seedlings
title_fullStr	Nitrogen deficiency results in changes to cell wall composition of sorghum seedlings
title_full_unstemmed	Nitrogen deficiency results in changes to cell wall composition of sorghum seedlings
title_sort	nitrogen deficiency results in changes to cell wall composition of sorghum seedlings
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/59220d95da344d25b22927cc1cb7e9d0
work_keys_str_mv	AT rezaramdanrivai nitrogendeficiencyresultsinchangestocellwallcompositionofsorghumseedlings AT takujimiyamoto nitrogendeficiencyresultsinchangestocellwallcompositionofsorghumseedlings AT tatsuyaawano nitrogendeficiencyresultsinchangestocellwallcompositionofsorghumseedlings AT rietakada nitrogendeficiencyresultsinchangestocellwallcompositionofsorghumseedlings AT yukitobimatsu nitrogendeficiencyresultsinchangestocellwallcompositionofsorghumseedlings AT toshiakiumezawa nitrogendeficiencyresultsinchangestocellwallcompositionofsorghumseedlings AT masarukobayashi nitrogendeficiencyresultsinchangestocellwallcompositionofsorghumseedlings
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Nitrogen deficiency results in changes to cell wall composition of sorghum seedlings

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