Assessment of the Antigenotoxic Effects of Alginate and ZnO/Alginate–Nanocomposites Extracted from Brown Alga <i>Fucus vesiculosus</i> in Mice

Mitomycin C (MMC) is an alkylating chemotherapy drug that could induce DNA damage and genetic alteration. It has been used as a model mutagen for in vivo and in vitro studies. The current study aimed to evaluate the protective role of Zinc oxide alginate–nanocomposites (ZnO-Alg/NCMs) against MMC–ind...

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Auteurs principaux:	Ragaa A. Hamouda, Asmaa S. Salman, Asrar A. Alharbi, Reem Hasaballah Alhasani, Maha M. Elshamy
Format:	article
Langue:	EN
Publié:	MDPI AG 2021
Sujets:	ZnO-Alg/NCMs alginate <i>Fucus vesiculosus alga</i> genotoxicity MMC Organic chemistry QD241-441
Accès en ligne:	https://doaj.org/article/5923d4d1a88b42ff8b9379b4cda0104f
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spelling	oai:doaj.org-article:5923d4d1a88b42ff8b9379b4cda0104f2021-11-11T18:48:42ZAssessment of the Antigenotoxic Effects of Alginate and ZnO/Alginate–Nanocomposites Extracted from Brown Alga <i>Fucus vesiculosus</i> in Mice10.3390/polym132138392073-4360https://doaj.org/article/5923d4d1a88b42ff8b9379b4cda0104f2021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4360/13/21/3839https://doaj.org/toc/2073-4360Mitomycin C (MMC) is an alkylating chemotherapy drug that could induce DNA damage and genetic alteration. It has been used as a model mutagen for in vivo and in vitro studies. The current study aimed to evaluate the protective role of Zinc oxide alginate–nanocomposites (ZnO-Alg/NCMs) against MMC–induced genotoxicity in mice. Animals were treated as follows: the control group, the groups treated with Algin (400 mg/kg b.w), the groups treated with ZnO-Alg/NCMs (400 mg/kg b.w), the group treated with MMC, and the groups treated with MMC plus Algin or ZnO-Alg/NCMs. Pre-treatment with Algin and ZnO-Alg/NCMs was repeated for one or seven days. Zinc oxide alginate-nanocomposites (ZnO-Alg/NCMs) were synthesized with the aim of incorporating the intrinsic properties of their constituents as an antigenotoxic substance. In this study, alginate was extracted from the brown marine alga <i>Fucus vesiculosus</i>, Zinc oxide nanoparticles were synthesized by using water extract of the same alga, and loaded in alginate to synthesize ZnO-Alg/NCMs. ZnO-NPs and ZnO-Alg/NCMs were characterized by TEM, SEM, EDX, and Zeta potential. The obtained results confirmed that by TEM and SEM, ZnO-NPs are rod shaped which modified, when loaded in alginate matrix, into spherical shape. The physical stability of ZnO-Alg/NCMs was reported to be higher than ZnO-NPs due to the presence of more negative charges on ZnO-Alg/NCMs. The EDX analysis indicated that the amount of zinc was higher in ZnO-NPs than ZnO-Alg/NCMs. The in vivo results showed that treatment with MMC induced genotoxic disturbances. The combined treatment with Algin and ZnO-Alg/NCMs succeeded in inducing significant protection against MMC. It could be concluded that ZnO-Algin/NCMs is a promising candidate to protect against MMC–induced genotoxicity.Ragaa A. HamoudaAsmaa S. SalmanAsrar A. AlharbiReem Hasaballah AlhasaniMaha M. ElshamyMDPI AGarticleZnO-Alg/NCMsalginate<i>Fucus vesiculosus alga</i>genotoxicityMMCOrganic chemistryQD241-441ENPolymers, Vol 13, Iss 3839, p 3839 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ZnO-Alg/NCMs alginate <i>Fucus vesiculosus alga</i> genotoxicity MMC Organic chemistry QD241-441
spellingShingle	ZnO-Alg/NCMs alginate <i>Fucus vesiculosus alga</i> genotoxicity MMC Organic chemistry QD241-441 Ragaa A. Hamouda Asmaa S. Salman Asrar A. Alharbi Reem Hasaballah Alhasani Maha M. Elshamy Assessment of the Antigenotoxic Effects of Alginate and ZnO/Alginate–Nanocomposites Extracted from Brown Alga <i>Fucus vesiculosus</i> in Mice
description	Mitomycin C (MMC) is an alkylating chemotherapy drug that could induce DNA damage and genetic alteration. It has been used as a model mutagen for in vivo and in vitro studies. The current study aimed to evaluate the protective role of Zinc oxide alginate–nanocomposites (ZnO-Alg/NCMs) against MMC–induced genotoxicity in mice. Animals were treated as follows: the control group, the groups treated with Algin (400 mg/kg b.w), the groups treated with ZnO-Alg/NCMs (400 mg/kg b.w), the group treated with MMC, and the groups treated with MMC plus Algin or ZnO-Alg/NCMs. Pre-treatment with Algin and ZnO-Alg/NCMs was repeated for one or seven days. Zinc oxide alginate-nanocomposites (ZnO-Alg/NCMs) were synthesized with the aim of incorporating the intrinsic properties of their constituents as an antigenotoxic substance. In this study, alginate was extracted from the brown marine alga <i>Fucus vesiculosus</i>, Zinc oxide nanoparticles were synthesized by using water extract of the same alga, and loaded in alginate to synthesize ZnO-Alg/NCMs. ZnO-NPs and ZnO-Alg/NCMs were characterized by TEM, SEM, EDX, and Zeta potential. The obtained results confirmed that by TEM and SEM, ZnO-NPs are rod shaped which modified, when loaded in alginate matrix, into spherical shape. The physical stability of ZnO-Alg/NCMs was reported to be higher than ZnO-NPs due to the presence of more negative charges on ZnO-Alg/NCMs. The EDX analysis indicated that the amount of zinc was higher in ZnO-NPs than ZnO-Alg/NCMs. The in vivo results showed that treatment with MMC induced genotoxic disturbances. The combined treatment with Algin and ZnO-Alg/NCMs succeeded in inducing significant protection against MMC. It could be concluded that ZnO-Algin/NCMs is a promising candidate to protect against MMC–induced genotoxicity.
format	article
author	Ragaa A. Hamouda Asmaa S. Salman Asrar A. Alharbi Reem Hasaballah Alhasani Maha M. Elshamy
author_facet	Ragaa A. Hamouda Asmaa S. Salman Asrar A. Alharbi Reem Hasaballah Alhasani Maha M. Elshamy
author_sort	Ragaa A. Hamouda
title	Assessment of the Antigenotoxic Effects of Alginate and ZnO/Alginate–Nanocomposites Extracted from Brown Alga <i>Fucus vesiculosus</i> in Mice
title_short	Assessment of the Antigenotoxic Effects of Alginate and ZnO/Alginate–Nanocomposites Extracted from Brown Alga <i>Fucus vesiculosus</i> in Mice
title_full	Assessment of the Antigenotoxic Effects of Alginate and ZnO/Alginate–Nanocomposites Extracted from Brown Alga <i>Fucus vesiculosus</i> in Mice
title_fullStr	Assessment of the Antigenotoxic Effects of Alginate and ZnO/Alginate–Nanocomposites Extracted from Brown Alga <i>Fucus vesiculosus</i> in Mice
title_full_unstemmed	Assessment of the Antigenotoxic Effects of Alginate and ZnO/Alginate–Nanocomposites Extracted from Brown Alga <i>Fucus vesiculosus</i> in Mice
title_sort	assessment of the antigenotoxic effects of alginate and zno/alginate–nanocomposites extracted from brown alga <i>fucus vesiculosus</i> in mice
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/5923d4d1a88b42ff8b9379b4cda0104f
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Assessment of the Antigenotoxic Effects of Alginate and ZnO/Alginate–Nanocomposites Extracted from Brown Alga <i>Fucus vesiculosus</i> in Mice

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