Histidine Promotes the Glucose Synthesis through Activation of the Gluconeogenic Pathway in Bovine Hepatocytes

Histidine (His) is considered to be the first-limiting amino acid (AA) on grass silage-based diets in lactation cows, which correlate positively with lactose yield. The higher glucose requirements of lactating cows can be met through a combination of increased capacity for gluconeogenesis and increa...

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Autores principales:	Tianyu Yang, Zhiqiang Cheng, Maocheng Jiang, Xiaoyu Ma, Osmond Datsomor, Guoqi Zhao, Kang Zhan
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	histidine bovine hepatocytes gluconeogenesis Veterinary medicine SF600-1100 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/59264fed9775401a854e6c408d941ec5
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spelling	oai:doaj.org-article:59264fed9775401a854e6c408d941ec52021-11-25T16:20:44ZHistidine Promotes the Glucose Synthesis through Activation of the Gluconeogenic Pathway in Bovine Hepatocytes10.3390/ani111132952076-2615https://doaj.org/article/59264fed9775401a854e6c408d941ec52021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3295https://doaj.org/toc/2076-2615Histidine (His) is considered to be the first-limiting amino acid (AA) on grass silage-based diets in lactation cows, which correlate positively with lactose yield. The higher glucose requirements of lactating cows can be met through a combination of increased capacity for gluconeogenesis and increased supply of gluconeogenic precursors. However, the effect of His on the expression of gluconeogenic genes in the bovine hepatocytes is less known. Therefore, this study aimed to investigate the regulatory effect of His on the key gluconeogenic genes and glucose output in bovine hepatocytes. The addition of 0.15, 0.6, and 1.2 mM His in a medium significantly enhanced (<i>p</i> < 0.05) the viability of bovine hepatocytes. Remarkably, 1.2 mM His induced profound changes (<i>p</i> < 0.05) in the mRNA level of key genes involved in gluconeogenesis, including PCK1, PCK2, FBP1, and G6PC in vitro. Furthermore, the mRNA expression of PCK1 was significantly elevated (<i>p</i> < 0.05) by the addition of 1.2 mM His at 3, 6, 12, and 24 h of incubation. The hepatic glucose output increased (<i>p</i> < 0.05) linearly with increasing His concentration. These findings indicate that the addition of His may be efficiently converted into glucose via the upregulation of genes related to the gluconeogenic pathway.Tianyu YangZhiqiang ChengMaocheng JiangXiaoyu MaOsmond DatsomorGuoqi ZhaoKang ZhanMDPI AGarticlehistidinebovine hepatocytesgluconeogenesisVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3295, p 3295 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	histidine bovine hepatocytes gluconeogenesis Veterinary medicine SF600-1100 Zoology QL1-991
spellingShingle	histidine bovine hepatocytes gluconeogenesis Veterinary medicine SF600-1100 Zoology QL1-991 Tianyu Yang Zhiqiang Cheng Maocheng Jiang Xiaoyu Ma Osmond Datsomor Guoqi Zhao Kang Zhan Histidine Promotes the Glucose Synthesis through Activation of the Gluconeogenic Pathway in Bovine Hepatocytes
description	Histidine (His) is considered to be the first-limiting amino acid (AA) on grass silage-based diets in lactation cows, which correlate positively with lactose yield. The higher glucose requirements of lactating cows can be met through a combination of increased capacity for gluconeogenesis and increased supply of gluconeogenic precursors. However, the effect of His on the expression of gluconeogenic genes in the bovine hepatocytes is less known. Therefore, this study aimed to investigate the regulatory effect of His on the key gluconeogenic genes and glucose output in bovine hepatocytes. The addition of 0.15, 0.6, and 1.2 mM His in a medium significantly enhanced (<i>p</i> < 0.05) the viability of bovine hepatocytes. Remarkably, 1.2 mM His induced profound changes (<i>p</i> < 0.05) in the mRNA level of key genes involved in gluconeogenesis, including PCK1, PCK2, FBP1, and G6PC in vitro. Furthermore, the mRNA expression of PCK1 was significantly elevated (<i>p</i> < 0.05) by the addition of 1.2 mM His at 3, 6, 12, and 24 h of incubation. The hepatic glucose output increased (<i>p</i> < 0.05) linearly with increasing His concentration. These findings indicate that the addition of His may be efficiently converted into glucose via the upregulation of genes related to the gluconeogenic pathway.
format	article
author	Tianyu Yang Zhiqiang Cheng Maocheng Jiang Xiaoyu Ma Osmond Datsomor Guoqi Zhao Kang Zhan
author_facet	Tianyu Yang Zhiqiang Cheng Maocheng Jiang Xiaoyu Ma Osmond Datsomor Guoqi Zhao Kang Zhan
author_sort	Tianyu Yang
title	Histidine Promotes the Glucose Synthesis through Activation of the Gluconeogenic Pathway in Bovine Hepatocytes
title_short	Histidine Promotes the Glucose Synthesis through Activation of the Gluconeogenic Pathway in Bovine Hepatocytes
title_full	Histidine Promotes the Glucose Synthesis through Activation of the Gluconeogenic Pathway in Bovine Hepatocytes
title_fullStr	Histidine Promotes the Glucose Synthesis through Activation of the Gluconeogenic Pathway in Bovine Hepatocytes
title_full_unstemmed	Histidine Promotes the Glucose Synthesis through Activation of the Gluconeogenic Pathway in Bovine Hepatocytes
title_sort	histidine promotes the glucose synthesis through activation of the gluconeogenic pathway in bovine hepatocytes
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/59264fed9775401a854e6c408d941ec5
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Histidine Promotes the Glucose Synthesis through Activation of the Gluconeogenic Pathway in Bovine Hepatocytes

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