Endotoxin tolerance variation over 24 h during porcine endotoxemia: association with changes in circulation and organ dysfunction.

Endotoxin tolerance (ET), defined as reduced inflammatory responsiveness to endotoxin challenge following a first encounter with endotoxin, is an extensively studied phenomenon. Although reduced mortality and morbidity in the presence of ET has been demonstrated in animal studies, little is known ab...

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Autores principales:	Markus Castegren, Paul Skorup, Miklós Lipcsey, Anders Larsson, Jan Sjölin
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5927cbb8a1624119b0c919a942173764
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spelling	oai:doaj.org-article:5927cbb8a1624119b0c919a9421737642021-11-18T08:02:03ZEndotoxin tolerance variation over 24 h during porcine endotoxemia: association with changes in circulation and organ dysfunction.1932-620310.1371/journal.pone.0053221https://doaj.org/article/5927cbb8a1624119b0c919a9421737642013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23326400/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Endotoxin tolerance (ET), defined as reduced inflammatory responsiveness to endotoxin challenge following a first encounter with endotoxin, is an extensively studied phenomenon. Although reduced mortality and morbidity in the presence of ET has been demonstrated in animal studies, little is known about the temporal development of ET. Further, in acute respiratory distress syndrome ET correlates to the severity of the disease, suggesting a complicated relation between ET and organ dysfunction. Eighteen pigs were subjected to intensive care and a continuous endotoxin infusion for 24 h with the aim to study the time course of early ET and to relate ET to outcome in organ dysfunction. Three animals served as non-endotoxemic controls. Blood samples for cytokine analyses were taken and physiological variables registered every third hour. Production of TNF-α, IL-6, and IL-10 before and after endotoxin stimulation ex vivo was measured. The difference between cytokine values after and before ex vivo LPS stimulation (Δ-values) was calculated for all time points. ΔTNF-α was employed as the principal marker of ET and lower ΔTNF-α values were interpreted as higher levels of ET. During endotoxin infusion, there was suppression of ex vivo productions of TNF-α and IL-6 but not of IL-10 in comparison with that at 0 h. The ex vivo TNF-α values followed another time concentration curve than those in vivo. ΔTNF-α was at the lowest already at 6 h, followed by an increase during the ensuing hours. ΔTNF-α at 6 h correlated positively to blood pressure and systemic vascular resistance and negatively to cardiac index at 24 h. In this study a temporal variation of ET was demonstrated that did not follow changes in plasma TNF-α concentrations. Maximal ET occurred early in the course and the higher the ET, the more hyperdynamic the circulation 18 h later.Markus CastegrenPaul SkorupMiklós LipcseyAnders LarssonJan SjölinPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 1, p e53221 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Markus Castegren Paul Skorup Miklós Lipcsey Anders Larsson Jan Sjölin Endotoxin tolerance variation over 24 h during porcine endotoxemia: association with changes in circulation and organ dysfunction.
description	Endotoxin tolerance (ET), defined as reduced inflammatory responsiveness to endotoxin challenge following a first encounter with endotoxin, is an extensively studied phenomenon. Although reduced mortality and morbidity in the presence of ET has been demonstrated in animal studies, little is known about the temporal development of ET. Further, in acute respiratory distress syndrome ET correlates to the severity of the disease, suggesting a complicated relation between ET and organ dysfunction. Eighteen pigs were subjected to intensive care and a continuous endotoxin infusion for 24 h with the aim to study the time course of early ET and to relate ET to outcome in organ dysfunction. Three animals served as non-endotoxemic controls. Blood samples for cytokine analyses were taken and physiological variables registered every third hour. Production of TNF-α, IL-6, and IL-10 before and after endotoxin stimulation ex vivo was measured. The difference between cytokine values after and before ex vivo LPS stimulation (Δ-values) was calculated for all time points. ΔTNF-α was employed as the principal marker of ET and lower ΔTNF-α values were interpreted as higher levels of ET. During endotoxin infusion, there was suppression of ex vivo productions of TNF-α and IL-6 but not of IL-10 in comparison with that at 0 h. The ex vivo TNF-α values followed another time concentration curve than those in vivo. ΔTNF-α was at the lowest already at 6 h, followed by an increase during the ensuing hours. ΔTNF-α at 6 h correlated positively to blood pressure and systemic vascular resistance and negatively to cardiac index at 24 h. In this study a temporal variation of ET was demonstrated that did not follow changes in plasma TNF-α concentrations. Maximal ET occurred early in the course and the higher the ET, the more hyperdynamic the circulation 18 h later.
format	article
author	Markus Castegren Paul Skorup Miklós Lipcsey Anders Larsson Jan Sjölin
author_facet	Markus Castegren Paul Skorup Miklós Lipcsey Anders Larsson Jan Sjölin
author_sort	Markus Castegren
title	Endotoxin tolerance variation over 24 h during porcine endotoxemia: association with changes in circulation and organ dysfunction.
title_short	Endotoxin tolerance variation over 24 h during porcine endotoxemia: association with changes in circulation and organ dysfunction.
title_full	Endotoxin tolerance variation over 24 h during porcine endotoxemia: association with changes in circulation and organ dysfunction.
title_fullStr	Endotoxin tolerance variation over 24 h during porcine endotoxemia: association with changes in circulation and organ dysfunction.
title_full_unstemmed	Endotoxin tolerance variation over 24 h during porcine endotoxemia: association with changes in circulation and organ dysfunction.
title_sort	endotoxin tolerance variation over 24 h during porcine endotoxemia: association with changes in circulation and organ dysfunction.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/5927cbb8a1624119b0c919a942173764
work_keys_str_mv	AT markuscastegren endotoxintolerancevariationover24hduringporcineendotoxemiaassociationwithchangesincirculationandorgandysfunction AT paulskorup endotoxintolerancevariationover24hduringporcineendotoxemiaassociationwithchangesincirculationandorgandysfunction AT mikloslipcsey endotoxintolerancevariationover24hduringporcineendotoxemiaassociationwithchangesincirculationandorgandysfunction AT anderslarsson endotoxintolerancevariationover24hduringporcineendotoxemiaassociationwithchangesincirculationandorgandysfunction AT jansjolin endotoxintolerancevariationover24hduringporcineendotoxemiaassociationwithchangesincirculationandorgandysfunction
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Endotoxin tolerance variation over 24 h during porcine endotoxemia: association with changes in circulation and organ dysfunction.

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