The association between DNA copy number aberrations at chromosome 5q22 and gastric cancer.

<h4>Background</h4>Gastric cancer is common cancer. Discovering novel genetic biomarkers might help to identify high-risk individuals. Copy number variation (CNV) has recently been shown to influence risk for several cancers. The aim of the present study was sought to test the associatio...

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Autores principales:	Pei-Chien Tsai, Szu-Wei Huang, Hsiang-Lin Tsai, Cheng-Jen Ma, Ming-Feng Hou, I-Ping Yang, Yung-Song Wang, Suh-Hang Hank Juo, Jaw-Yuan Wang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/592a7fa51a0149129f2d64967d6fcc5a
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spelling	oai:doaj.org-article:592a7fa51a0149129f2d64967d6fcc5a2021-11-25T06:01:00ZThe association between DNA copy number aberrations at chromosome 5q22 and gastric cancer.1932-620310.1371/journal.pone.0106624https://doaj.org/article/592a7fa51a0149129f2d64967d6fcc5a2014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0106624https://doaj.org/toc/1932-6203<h4>Background</h4>Gastric cancer is common cancer. Discovering novel genetic biomarkers might help to identify high-risk individuals. Copy number variation (CNV) has recently been shown to influence risk for several cancers. The aim of the present study was sought to test the association between copy number at a variant region and GC.<h4>Methods</h4>A total of 110 gastric cancer patients and 325 healthy volunteers were enrolled in this study. We searched for a CNV and found a CNV (Variation 7468) containing part of the APC gene, the SRP19 gene and the REEP5 gene. We chose four probes targeting at APC-intron8, APC-exon9, SRP19 and REEP5 to interrogate this CNV. Specific Taqman probes labeled by different reporter fluorophores were used in a real-time PCR platform to obtain copy number. Both the original non-integer data and transformed integer data on copy number were used for analyses.<h4>Results</h4>Gastric caner patients had a lower non-integer copy number than controls for the APC-exon9 probe (Adjusted p = 0.026) and SRP19 probe (Adjusted p = 0.002). The analysis of integer copy number yielded a similar pattern although less significant (Adjusted p = 0.07 for APC-exon9 probe and Adjusted p = 0.02 for SRP19 probe).<h4>Conclusions</h4>Losses of a CNV at 5q22, especially in the DNA region surrounding APC-exon 9, may be associated with a higher risk of gastric cancer.Pei-Chien TsaiSzu-Wei HuangHsiang-Lin TsaiCheng-Jen MaMing-Feng HouI-Ping YangYung-Song WangSuh-Hang Hank JuoJaw-Yuan WangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 9, p e106624 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pei-Chien Tsai Szu-Wei Huang Hsiang-Lin Tsai Cheng-Jen Ma Ming-Feng Hou I-Ping Yang Yung-Song Wang Suh-Hang Hank Juo Jaw-Yuan Wang The association between DNA copy number aberrations at chromosome 5q22 and gastric cancer.
description	<h4>Background</h4>Gastric cancer is common cancer. Discovering novel genetic biomarkers might help to identify high-risk individuals. Copy number variation (CNV) has recently been shown to influence risk for several cancers. The aim of the present study was sought to test the association between copy number at a variant region and GC.<h4>Methods</h4>A total of 110 gastric cancer patients and 325 healthy volunteers were enrolled in this study. We searched for a CNV and found a CNV (Variation 7468) containing part of the APC gene, the SRP19 gene and the REEP5 gene. We chose four probes targeting at APC-intron8, APC-exon9, SRP19 and REEP5 to interrogate this CNV. Specific Taqman probes labeled by different reporter fluorophores were used in a real-time PCR platform to obtain copy number. Both the original non-integer data and transformed integer data on copy number were used for analyses.<h4>Results</h4>Gastric caner patients had a lower non-integer copy number than controls for the APC-exon9 probe (Adjusted p = 0.026) and SRP19 probe (Adjusted p = 0.002). The analysis of integer copy number yielded a similar pattern although less significant (Adjusted p = 0.07 for APC-exon9 probe and Adjusted p = 0.02 for SRP19 probe).<h4>Conclusions</h4>Losses of a CNV at 5q22, especially in the DNA region surrounding APC-exon 9, may be associated with a higher risk of gastric cancer.
format	article
author	Pei-Chien Tsai Szu-Wei Huang Hsiang-Lin Tsai Cheng-Jen Ma Ming-Feng Hou I-Ping Yang Yung-Song Wang Suh-Hang Hank Juo Jaw-Yuan Wang
author_facet	Pei-Chien Tsai Szu-Wei Huang Hsiang-Lin Tsai Cheng-Jen Ma Ming-Feng Hou I-Ping Yang Yung-Song Wang Suh-Hang Hank Juo Jaw-Yuan Wang
author_sort	Pei-Chien Tsai
title	The association between DNA copy number aberrations at chromosome 5q22 and gastric cancer.
title_short	The association between DNA copy number aberrations at chromosome 5q22 and gastric cancer.
title_full	The association between DNA copy number aberrations at chromosome 5q22 and gastric cancer.
title_fullStr	The association between DNA copy number aberrations at chromosome 5q22 and gastric cancer.
title_full_unstemmed	The association between DNA copy number aberrations at chromosome 5q22 and gastric cancer.
title_sort	association between dna copy number aberrations at chromosome 5q22 and gastric cancer.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/592a7fa51a0149129f2d64967d6fcc5a
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The association between DNA copy number aberrations at chromosome 5q22 and gastric cancer.

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