16S rRNA terminal restriction fragment length polymorphism for the characterization of the nasopharyngeal microbiota.

16S rRNA terminal restriction fragment length polymorphism for the characterization of the nasopharyngeal microbiota.

A novel non-culture based 16S rRNA Terminal Restriction Fragment Length Polymorphism (T-RFLP) method using the restriction enzymes Tsp509I and Hpy166II was developed for the characterization of the nasopharyngeal microbiota and validated using recently published 454 pyrosequencing data. 16S rRNA gen...

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Bibliographic Details
Main Authors: Silvio D Brugger, Laurence Frei, Pascal M Frey, Suzanne Aebi, Kathrin Mühlemann, Markus Hilty
Format: article
Language:EN
Published: Public Library of Science (PLoS) 2012
Subjects:
Medicine
R
Science
Q
Online Access:https://doaj.org/article/59329a28c8584c69b9fca502c7220b6c
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