RSK2 is a modulator of craniofacial development.

<h4>Background</h4>The RSK2 gene is responsible for Coffin-Lowry syndrome, an X-linked dominant genetic disorder causing mental retardation, skeletal growth delays, with craniofacial and digital abnormalities typically associated with this syndrome. Craniofacial and dental anomalies enco...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Virginie Laugel-Haushalter, Marie Paschaki, Pauline Marangoni, Coralie Pilgram, Arnaud Langer, Thibaut Kuntz, Julie Demassue, Supawich Morkmued, Philippe Choquet, André Constantinesco, Fabien Bornert, Matthieu Schmittbuhl, Solange Pannetier, Laurent Viriot, André Hanauer, Pascal Dollé, Agnès Bloch-Zupan
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2014
Materias:
R
Q
Acceso en línea:https://doaj.org/article/59354c677b234ef4aa0e1bfbf8185d97
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:59354c677b234ef4aa0e1bfbf8185d97
record_format dspace
spelling oai:doaj.org-article:59354c677b234ef4aa0e1bfbf8185d972021-11-18T08:38:35ZRSK2 is a modulator of craniofacial development.1932-620310.1371/journal.pone.0084343https://doaj.org/article/59354c677b234ef4aa0e1bfbf8185d972014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24416220/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The RSK2 gene is responsible for Coffin-Lowry syndrome, an X-linked dominant genetic disorder causing mental retardation, skeletal growth delays, with craniofacial and digital abnormalities typically associated with this syndrome. Craniofacial and dental anomalies encountered in this rare disease have been poorly characterized.<h4>Methodology/principal findings</h4>We examined, using X-Ray microtomographic analysis, the variable craniofacial dysmorphism and dental anomalies present in Rsk2 knockout mice, a model of Coffin-Lowry syndrome, as well as in triple Rsk1,2,3 knockout mutants. We report Rsk mutation produces surpernumerary teeth midline/mesial to the first molar. This highly penetrant phenotype recapitulates more ancestral tooth structures lost with evolution. Most likely this leads to a reduction of the maxillary diastema. Abnormalities of molar shape were generally restricted to the mesial part of both upper and lower first molars (M1). Expression analysis of the four Rsk genes (Rsk1, 2, 3 and 4) was performed at various stages of odontogenesis in wild-type (WT) mice. Rsk2 is expressed in the mesenchymal, neural crest-derived compartment, correlating with proliferative areas of the developing teeth. This is consistent with RSK2 functioning in cell cycle control and growth regulation, functions potentially responsible for severe dental phenotypes. To uncover molecular pathways involved in the etiology of these defects, we performed a comparative transcriptomic (DNA microarray) analysis of mandibular wild-type versus Rsk2-/Y molars. We further demonstrated a misregulation of several critical genes, using a Rsk2 shRNA knock-down strategy in molar tooth germs cultured in vitro.<h4>Conclusions</h4>This study reveals RSK2 regulates craniofacial development including tooth development and patterning via novel transcriptional targets.Virginie Laugel-HaushalterMarie PaschakiPauline MarangoniCoralie PilgramArnaud LangerThibaut KuntzJulie DemassueSupawich MorkmuedPhilippe ChoquetAndré ConstantinescoFabien BornertMatthieu SchmittbuhlSolange PannetierLaurent ViriotAndré HanauerPascal DolléAgnès Bloch-ZupanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e84343 (2014)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Virginie Laugel-Haushalter
Marie Paschaki
Pauline Marangoni
Coralie Pilgram
Arnaud Langer
Thibaut Kuntz
Julie Demassue
Supawich Morkmued
Philippe Choquet
André Constantinesco
Fabien Bornert
Matthieu Schmittbuhl
Solange Pannetier
Laurent Viriot
André Hanauer
Pascal Dollé
Agnès Bloch-Zupan
RSK2 is a modulator of craniofacial development.
description <h4>Background</h4>The RSK2 gene is responsible for Coffin-Lowry syndrome, an X-linked dominant genetic disorder causing mental retardation, skeletal growth delays, with craniofacial and digital abnormalities typically associated with this syndrome. Craniofacial and dental anomalies encountered in this rare disease have been poorly characterized.<h4>Methodology/principal findings</h4>We examined, using X-Ray microtomographic analysis, the variable craniofacial dysmorphism and dental anomalies present in Rsk2 knockout mice, a model of Coffin-Lowry syndrome, as well as in triple Rsk1,2,3 knockout mutants. We report Rsk mutation produces surpernumerary teeth midline/mesial to the first molar. This highly penetrant phenotype recapitulates more ancestral tooth structures lost with evolution. Most likely this leads to a reduction of the maxillary diastema. Abnormalities of molar shape were generally restricted to the mesial part of both upper and lower first molars (M1). Expression analysis of the four Rsk genes (Rsk1, 2, 3 and 4) was performed at various stages of odontogenesis in wild-type (WT) mice. Rsk2 is expressed in the mesenchymal, neural crest-derived compartment, correlating with proliferative areas of the developing teeth. This is consistent with RSK2 functioning in cell cycle control and growth regulation, functions potentially responsible for severe dental phenotypes. To uncover molecular pathways involved in the etiology of these defects, we performed a comparative transcriptomic (DNA microarray) analysis of mandibular wild-type versus Rsk2-/Y molars. We further demonstrated a misregulation of several critical genes, using a Rsk2 shRNA knock-down strategy in molar tooth germs cultured in vitro.<h4>Conclusions</h4>This study reveals RSK2 regulates craniofacial development including tooth development and patterning via novel transcriptional targets.
format article
author Virginie Laugel-Haushalter
Marie Paschaki
Pauline Marangoni
Coralie Pilgram
Arnaud Langer
Thibaut Kuntz
Julie Demassue
Supawich Morkmued
Philippe Choquet
André Constantinesco
Fabien Bornert
Matthieu Schmittbuhl
Solange Pannetier
Laurent Viriot
André Hanauer
Pascal Dollé
Agnès Bloch-Zupan
author_facet Virginie Laugel-Haushalter
Marie Paschaki
Pauline Marangoni
Coralie Pilgram
Arnaud Langer
Thibaut Kuntz
Julie Demassue
Supawich Morkmued
Philippe Choquet
André Constantinesco
Fabien Bornert
Matthieu Schmittbuhl
Solange Pannetier
Laurent Viriot
André Hanauer
Pascal Dollé
Agnès Bloch-Zupan
author_sort Virginie Laugel-Haushalter
title RSK2 is a modulator of craniofacial development.
title_short RSK2 is a modulator of craniofacial development.
title_full RSK2 is a modulator of craniofacial development.
title_fullStr RSK2 is a modulator of craniofacial development.
title_full_unstemmed RSK2 is a modulator of craniofacial development.
title_sort rsk2 is a modulator of craniofacial development.
publisher Public Library of Science (PLoS)
publishDate 2014
url https://doaj.org/article/59354c677b234ef4aa0e1bfbf8185d97
work_keys_str_mv AT virginielaugelhaushalter rsk2isamodulatorofcraniofacialdevelopment
AT mariepaschaki rsk2isamodulatorofcraniofacialdevelopment
AT paulinemarangoni rsk2isamodulatorofcraniofacialdevelopment
AT coraliepilgram rsk2isamodulatorofcraniofacialdevelopment
AT arnaudlanger rsk2isamodulatorofcraniofacialdevelopment
AT thibautkuntz rsk2isamodulatorofcraniofacialdevelopment
AT juliedemassue rsk2isamodulatorofcraniofacialdevelopment
AT supawichmorkmued rsk2isamodulatorofcraniofacialdevelopment
AT philippechoquet rsk2isamodulatorofcraniofacialdevelopment
AT andreconstantinesco rsk2isamodulatorofcraniofacialdevelopment
AT fabienbornert rsk2isamodulatorofcraniofacialdevelopment
AT matthieuschmittbuhl rsk2isamodulatorofcraniofacialdevelopment
AT solangepannetier rsk2isamodulatorofcraniofacialdevelopment
AT laurentviriot rsk2isamodulatorofcraniofacialdevelopment
AT andrehanauer rsk2isamodulatorofcraniofacialdevelopment
AT pascaldolle rsk2isamodulatorofcraniofacialdevelopment
AT agnesblochzupan rsk2isamodulatorofcraniofacialdevelopment
_version_ 1718421502082154496