Determining the Efficacy of a Hybridizing Agent in Wheat (Triticum aestivum L.)

Abstract Hybrid wheat (Triticum spp.) has the potential to boost yields and enhance production under changing climates to feed the growing global population. Production of hybrid wheat seed relies on male sterility, the blocking of pollen production, to prevent self-pollination. One method of preven...

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Autores principales:	Amanda C. Easterly, Walter W. Stroup, Nicholas Garst, Vikas Belamkar, Jean-Benoit Sarazin, Thierry Moittié, Amir M. H. Ibrahim, Jackie C. Rudd, Edward Souza, P. Stephen Baenziger
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/5939133403dc42bfa77a2d9d268d2081
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spelling	oai:doaj.org-article:5939133403dc42bfa77a2d9d268d20812021-12-02T13:34:55ZDetermining the Efficacy of a Hybridizing Agent in Wheat (Triticum aestivum L.)10.1038/s41598-019-56664-92045-2322https://doaj.org/article/5939133403dc42bfa77a2d9d268d20812019-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-56664-9https://doaj.org/toc/2045-2322Abstract Hybrid wheat (Triticum spp.) has the potential to boost yields and enhance production under changing climates to feed the growing global population. Production of hybrid wheat seed relies on male sterility, the blocking of pollen production, to prevent self-pollination. One method of preventing self-pollination in the female plants is to apply a chemical hybridizing agent (CHA). However, some combinations of CHA and genotypes have lower levels of sterility, resulting in decreased hybrid purity. Differences in CHA efficacy are a challenge in producing hybrid wheat lines for commercial and experimental use. Our primary research questions were to estimate the levels of sterility for wheat genotypes treated with a CHA and determine the best way to analyze differences. We applied the CHA sintofen (1-(4-chlorphyl)-1,4-dihydro-5-(2-methoxyethoxy)-4-oxocinnoline-3-carboxylic acid; Croisor 100) to 27 genotypes in replicate. After spraying, we counted seed in bagged female heads to evaluate CHA efficacy and CHA-by-genotype interaction. Using logit and probit models with a threshold of 7 seeds, we found differences among genotypes in 2015. Sterility was higher in 2016 and fewer genotypic differences were found. When CHA-induced sterilization is less uniform as in 2015, zero-inflated and hurdle count models were superior to standard mixed models. These models calculate mean seed number and fit data with limit-bounded scales collected by agronomists and plant breeders to compare genotypic differences. These analyses can assist in selecting parents and identifying where additional optimization of CHA application needs to occur. There is little work in the literature examining the relationship between CHAs and genotypes, making this work fundamental to the future of hybrid wheat breeding.Amanda C. EasterlyWalter W. StroupNicholas GarstVikas BelamkarJean-Benoit SarazinThierry MoittiéAmir M. H. IbrahimJackie C. RuddEdward SouzaP. Stephen BaenzigerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-11 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Amanda C. Easterly Walter W. Stroup Nicholas Garst Vikas Belamkar Jean-Benoit Sarazin Thierry Moittié Amir M. H. Ibrahim Jackie C. Rudd Edward Souza P. Stephen Baenziger Determining the Efficacy of a Hybridizing Agent in Wheat (Triticum aestivum L.)
description	Abstract Hybrid wheat (Triticum spp.) has the potential to boost yields and enhance production under changing climates to feed the growing global population. Production of hybrid wheat seed relies on male sterility, the blocking of pollen production, to prevent self-pollination. One method of preventing self-pollination in the female plants is to apply a chemical hybridizing agent (CHA). However, some combinations of CHA and genotypes have lower levels of sterility, resulting in decreased hybrid purity. Differences in CHA efficacy are a challenge in producing hybrid wheat lines for commercial and experimental use. Our primary research questions were to estimate the levels of sterility for wheat genotypes treated with a CHA and determine the best way to analyze differences. We applied the CHA sintofen (1-(4-chlorphyl)-1,4-dihydro-5-(2-methoxyethoxy)-4-oxocinnoline-3-carboxylic acid; Croisor 100) to 27 genotypes in replicate. After spraying, we counted seed in bagged female heads to evaluate CHA efficacy and CHA-by-genotype interaction. Using logit and probit models with a threshold of 7 seeds, we found differences among genotypes in 2015. Sterility was higher in 2016 and fewer genotypic differences were found. When CHA-induced sterilization is less uniform as in 2015, zero-inflated and hurdle count models were superior to standard mixed models. These models calculate mean seed number and fit data with limit-bounded scales collected by agronomists and plant breeders to compare genotypic differences. These analyses can assist in selecting parents and identifying where additional optimization of CHA application needs to occur. There is little work in the literature examining the relationship between CHAs and genotypes, making this work fundamental to the future of hybrid wheat breeding.
format	article
author	Amanda C. Easterly Walter W. Stroup Nicholas Garst Vikas Belamkar Jean-Benoit Sarazin Thierry Moittié Amir M. H. Ibrahim Jackie C. Rudd Edward Souza P. Stephen Baenziger
author_facet	Amanda C. Easterly Walter W. Stroup Nicholas Garst Vikas Belamkar Jean-Benoit Sarazin Thierry Moittié Amir M. H. Ibrahim Jackie C. Rudd Edward Souza P. Stephen Baenziger
author_sort	Amanda C. Easterly
title	Determining the Efficacy of a Hybridizing Agent in Wheat (Triticum aestivum L.)
title_short	Determining the Efficacy of a Hybridizing Agent in Wheat (Triticum aestivum L.)
title_full	Determining the Efficacy of a Hybridizing Agent in Wheat (Triticum aestivum L.)
title_fullStr	Determining the Efficacy of a Hybridizing Agent in Wheat (Triticum aestivum L.)
title_full_unstemmed	Determining the Efficacy of a Hybridizing Agent in Wheat (Triticum aestivum L.)
title_sort	determining the efficacy of a hybridizing agent in wheat (triticum aestivum l.)
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/5939133403dc42bfa77a2d9d268d2081
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Determining the Efficacy of a Hybridizing Agent in Wheat (Triticum aestivum L.)

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