Contribution of multiparameter flow cytometry immunophenotyping to the diagnostic screening and classification of pediatric cancer.
Pediatric cancer is a relatively rare and heterogeneous group of hematological and non-hematological malignancies which require multiple procedures for its diagnostic screening and classification. Until now, flow cytometry (FC) has not been systematically applied to the diagnostic work-up of such ma...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2013
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/593fb01905c24092b3ea00ea295c75a7 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:593fb01905c24092b3ea00ea295c75a7 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:593fb01905c24092b3ea00ea295c75a72021-11-18T07:54:59ZContribution of multiparameter flow cytometry immunophenotyping to the diagnostic screening and classification of pediatric cancer.1932-620310.1371/journal.pone.0055534https://doaj.org/article/593fb01905c24092b3ea00ea295c75a72013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23472067/?tool=EBIhttps://doaj.org/toc/1932-6203Pediatric cancer is a relatively rare and heterogeneous group of hematological and non-hematological malignancies which require multiple procedures for its diagnostic screening and classification. Until now, flow cytometry (FC) has not been systematically applied to the diagnostic work-up of such malignancies, particularly for solid tumors. Here we evaluated a FC panel of markers for the diagnostic screening of pediatric cancer and further classification of pediatric solid tumors. The proposed strategy aims at the differential diagnosis between tumoral vs. reactive samples, and hematological vs. non-hematological malignancies, and the subclassification of solid tumors. In total, 52 samples from 40 patients suspicious of containing tumor cells were analyzed by FC in parallel to conventional diagnostic procedures. The overall concordance rate between both approaches was of 96% (50/52 diagnostic samples), with 100% agreement for all reactive/inflammatory and non-infiltrated samples as well as for those corresponding to solid tumors (n = 35), with only two false negative cases diagnosed with Hodgkin lymphoma and anaplastic lymphoma, respectively. Moreover, clear discrimination between samples infiltrated by hematopoietic vs. non-hematopoietic tumor cells was systematically achieved. Distinct subtypes of solid tumors showed different protein expression profiles, allowing for the differential diagnosis of neuroblastoma (CD56(hi)/GD2(+)/CD81(hi)), primitive neuroectodermal tumors (CD271(hi)/CD99(+)), Wilms tumors (>1 cell population), rhabdomyosarcoma (nuMYOD1(+)/numyogenin(+)), carcinomas (CD45(-)/EpCAM(+)), germ cell tumors (CD56(+)/CD45(-)/NG2(+)/CD10(+)) and eventually also hemangiopericytomas (CD45(-)/CD34(+)). In summary, our results show that multiparameter FC provides fast and useful complementary data to routine histopathology for the diagnostic screening and classification of pediatric cancer.Cristiane S Ferreira-FacioCristiane MilitoVitor BotafogoMarcela FontanaLeandro S ThiagoElen OliveiraAriovaldo S da Rocha-FilhoFernando WerneckDanielle N FornySamuel DekermacherAna Paula de AzambujaSima Esther FermanPaulo Antônio Silvestre de FariaMarcelo G P LandAlberto OrfaoElaine S CostaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 3, p e55534 (2013) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Cristiane S Ferreira-Facio Cristiane Milito Vitor Botafogo Marcela Fontana Leandro S Thiago Elen Oliveira Ariovaldo S da Rocha-Filho Fernando Werneck Danielle N Forny Samuel Dekermacher Ana Paula de Azambuja Sima Esther Ferman Paulo Antônio Silvestre de Faria Marcelo G P Land Alberto Orfao Elaine S Costa Contribution of multiparameter flow cytometry immunophenotyping to the diagnostic screening and classification of pediatric cancer. |
| description |
Pediatric cancer is a relatively rare and heterogeneous group of hematological and non-hematological malignancies which require multiple procedures for its diagnostic screening and classification. Until now, flow cytometry (FC) has not been systematically applied to the diagnostic work-up of such malignancies, particularly for solid tumors. Here we evaluated a FC panel of markers for the diagnostic screening of pediatric cancer and further classification of pediatric solid tumors. The proposed strategy aims at the differential diagnosis between tumoral vs. reactive samples, and hematological vs. non-hematological malignancies, and the subclassification of solid tumors. In total, 52 samples from 40 patients suspicious of containing tumor cells were analyzed by FC in parallel to conventional diagnostic procedures. The overall concordance rate between both approaches was of 96% (50/52 diagnostic samples), with 100% agreement for all reactive/inflammatory and non-infiltrated samples as well as for those corresponding to solid tumors (n = 35), with only two false negative cases diagnosed with Hodgkin lymphoma and anaplastic lymphoma, respectively. Moreover, clear discrimination between samples infiltrated by hematopoietic vs. non-hematopoietic tumor cells was systematically achieved. Distinct subtypes of solid tumors showed different protein expression profiles, allowing for the differential diagnosis of neuroblastoma (CD56(hi)/GD2(+)/CD81(hi)), primitive neuroectodermal tumors (CD271(hi)/CD99(+)), Wilms tumors (>1 cell population), rhabdomyosarcoma (nuMYOD1(+)/numyogenin(+)), carcinomas (CD45(-)/EpCAM(+)), germ cell tumors (CD56(+)/CD45(-)/NG2(+)/CD10(+)) and eventually also hemangiopericytomas (CD45(-)/CD34(+)). In summary, our results show that multiparameter FC provides fast and useful complementary data to routine histopathology for the diagnostic screening and classification of pediatric cancer. |
| format |
article |
| author |
Cristiane S Ferreira-Facio Cristiane Milito Vitor Botafogo Marcela Fontana Leandro S Thiago Elen Oliveira Ariovaldo S da Rocha-Filho Fernando Werneck Danielle N Forny Samuel Dekermacher Ana Paula de Azambuja Sima Esther Ferman Paulo Antônio Silvestre de Faria Marcelo G P Land Alberto Orfao Elaine S Costa |
| author_facet |
Cristiane S Ferreira-Facio Cristiane Milito Vitor Botafogo Marcela Fontana Leandro S Thiago Elen Oliveira Ariovaldo S da Rocha-Filho Fernando Werneck Danielle N Forny Samuel Dekermacher Ana Paula de Azambuja Sima Esther Ferman Paulo Antônio Silvestre de Faria Marcelo G P Land Alberto Orfao Elaine S Costa |
| author_sort |
Cristiane S Ferreira-Facio |
| title |
Contribution of multiparameter flow cytometry immunophenotyping to the diagnostic screening and classification of pediatric cancer. |
| title_short |
Contribution of multiparameter flow cytometry immunophenotyping to the diagnostic screening and classification of pediatric cancer. |
| title_full |
Contribution of multiparameter flow cytometry immunophenotyping to the diagnostic screening and classification of pediatric cancer. |
| title_fullStr |
Contribution of multiparameter flow cytometry immunophenotyping to the diagnostic screening and classification of pediatric cancer. |
| title_full_unstemmed |
Contribution of multiparameter flow cytometry immunophenotyping to the diagnostic screening and classification of pediatric cancer. |
| title_sort |
contribution of multiparameter flow cytometry immunophenotyping to the diagnostic screening and classification of pediatric cancer. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/593fb01905c24092b3ea00ea295c75a7 |
| work_keys_str_mv |
AT cristianesferreirafacio contributionofmultiparameterflowcytometryimmunophenotypingtothediagnosticscreeningandclassificationofpediatriccancer AT cristianemilito contributionofmultiparameterflowcytometryimmunophenotypingtothediagnosticscreeningandclassificationofpediatriccancer AT vitorbotafogo contributionofmultiparameterflowcytometryimmunophenotypingtothediagnosticscreeningandclassificationofpediatriccancer AT marcelafontana contributionofmultiparameterflowcytometryimmunophenotypingtothediagnosticscreeningandclassificationofpediatriccancer AT leandrosthiago contributionofmultiparameterflowcytometryimmunophenotypingtothediagnosticscreeningandclassificationofpediatriccancer AT elenoliveira contributionofmultiparameterflowcytometryimmunophenotypingtothediagnosticscreeningandclassificationofpediatriccancer AT ariovaldosdarochafilho contributionofmultiparameterflowcytometryimmunophenotypingtothediagnosticscreeningandclassificationofpediatriccancer AT fernandowerneck contributionofmultiparameterflowcytometryimmunophenotypingtothediagnosticscreeningandclassificationofpediatriccancer AT daniellenforny contributionofmultiparameterflowcytometryimmunophenotypingtothediagnosticscreeningandclassificationofpediatriccancer AT samueldekermacher contributionofmultiparameterflowcytometryimmunophenotypingtothediagnosticscreeningandclassificationofpediatriccancer AT anapauladeazambuja contributionofmultiparameterflowcytometryimmunophenotypingtothediagnosticscreeningandclassificationofpediatriccancer AT simaestherferman contributionofmultiparameterflowcytometryimmunophenotypingtothediagnosticscreeningandclassificationofpediatriccancer AT pauloantoniosilvestredefaria contributionofmultiparameterflowcytometryimmunophenotypingtothediagnosticscreeningandclassificationofpediatriccancer AT marcelogpland contributionofmultiparameterflowcytometryimmunophenotypingtothediagnosticscreeningandclassificationofpediatriccancer AT albertoorfao contributionofmultiparameterflowcytometryimmunophenotypingtothediagnosticscreeningandclassificationofpediatriccancer AT elainescosta contributionofmultiparameterflowcytometryimmunophenotypingtothediagnosticscreeningandclassificationofpediatriccancer |
| _version_ |
1718422730214211584 |