Involvement of oxidative modification of proteins related to ATP synthesis in the left ventricles of hamsters with cardiomyopathy

Abstract Inflammation enhanced by accumulation of reactive oxygen species plays an essential role in the progression of cardiovascular diseases. Using the 2D-oxyblot analysis and 2D-difference image gel electrophoresis (2D-DIGE), we compared the levels of ROS-induced carbonyl modification of myocard...

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Autores principales:	Sahoko Ichihara, Yuka Suzuki, Jie Chang, Kentaro Kuzuya, Chisa Inoue, Yuki Kitamura, Shinji Oikawa
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/59415c078eb549519737283144b3bd14
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spelling	oai:doaj.org-article:59415c078eb549519737283144b3bd142021-12-02T11:52:35ZInvolvement of oxidative modification of proteins related to ATP synthesis in the left ventricles of hamsters with cardiomyopathy10.1038/s41598-017-08546-12045-2322https://doaj.org/article/59415c078eb549519737283144b3bd142017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08546-1https://doaj.org/toc/2045-2322Abstract Inflammation enhanced by accumulation of reactive oxygen species plays an essential role in the progression of cardiovascular diseases. Using the 2D-oxyblot analysis and 2D-difference image gel electrophoresis (2D-DIGE), we compared the levels of ROS-induced carbonyl modification of myocardial proteins in the whole left ventricles between 6-week-old hamsters with dilated (TO-2) and hypertrophic cardiomyopathy (Bio14.6) and control hamsters (F1B). Then, 2D electrophoresis combined with MALDI-TOF/TOF tandem mass spectrometry detected 18 proteins with increased carbonyl level in cardiomyopathy hamsters compared with control hamster. Carbonyl modification of proteins related to ATP synthesis, including citric acid cycle and electron transport system, was observed in the hearts of hamsters with both types of cardiomyopathy. Further analysis indicated that left ventricular carbonyl production correlated negatively with succinyl-CoA:3-ketoacid-coenzyme A transferase 1 activity (r 2 = 0.60, P = 0.0007) and ATP concentration (r 2 = 0.29, P = 0.037), suggesting that protein carbonylation has negative effects on the levels of these biomolecules. Furthermore, carbonyl production significantly correlated with plasma Troponin T level (r 2 = 0.33, P = 0.026). Reduction of energy metabolism by oxidative damage may contribute to the development of left ventricular impairment in cardiomyopathy.Sahoko IchiharaYuka SuzukiJie ChangKentaro KuzuyaChisa InoueYuki KitamuraShinji OikawaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sahoko Ichihara Yuka Suzuki Jie Chang Kentaro Kuzuya Chisa Inoue Yuki Kitamura Shinji Oikawa Involvement of oxidative modification of proteins related to ATP synthesis in the left ventricles of hamsters with cardiomyopathy
description	Abstract Inflammation enhanced by accumulation of reactive oxygen species plays an essential role in the progression of cardiovascular diseases. Using the 2D-oxyblot analysis and 2D-difference image gel electrophoresis (2D-DIGE), we compared the levels of ROS-induced carbonyl modification of myocardial proteins in the whole left ventricles between 6-week-old hamsters with dilated (TO-2) and hypertrophic cardiomyopathy (Bio14.6) and control hamsters (F1B). Then, 2D electrophoresis combined with MALDI-TOF/TOF tandem mass spectrometry detected 18 proteins with increased carbonyl level in cardiomyopathy hamsters compared with control hamster. Carbonyl modification of proteins related to ATP synthesis, including citric acid cycle and electron transport system, was observed in the hearts of hamsters with both types of cardiomyopathy. Further analysis indicated that left ventricular carbonyl production correlated negatively with succinyl-CoA:3-ketoacid-coenzyme A transferase 1 activity (r 2 = 0.60, P = 0.0007) and ATP concentration (r 2 = 0.29, P = 0.037), suggesting that protein carbonylation has negative effects on the levels of these biomolecules. Furthermore, carbonyl production significantly correlated with plasma Troponin T level (r 2 = 0.33, P = 0.026). Reduction of energy metabolism by oxidative damage may contribute to the development of left ventricular impairment in cardiomyopathy.
format	article
author	Sahoko Ichihara Yuka Suzuki Jie Chang Kentaro Kuzuya Chisa Inoue Yuki Kitamura Shinji Oikawa
author_facet	Sahoko Ichihara Yuka Suzuki Jie Chang Kentaro Kuzuya Chisa Inoue Yuki Kitamura Shinji Oikawa
author_sort	Sahoko Ichihara
title	Involvement of oxidative modification of proteins related to ATP synthesis in the left ventricles of hamsters with cardiomyopathy
title_short	Involvement of oxidative modification of proteins related to ATP synthesis in the left ventricles of hamsters with cardiomyopathy
title_full	Involvement of oxidative modification of proteins related to ATP synthesis in the left ventricles of hamsters with cardiomyopathy
title_fullStr	Involvement of oxidative modification of proteins related to ATP synthesis in the left ventricles of hamsters with cardiomyopathy
title_full_unstemmed	Involvement of oxidative modification of proteins related to ATP synthesis in the left ventricles of hamsters with cardiomyopathy
title_sort	involvement of oxidative modification of proteins related to atp synthesis in the left ventricles of hamsters with cardiomyopathy
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/59415c078eb549519737283144b3bd14
work_keys_str_mv	AT sahokoichihara involvementofoxidativemodificationofproteinsrelatedtoatpsynthesisintheleftventriclesofhamsterswithcardiomyopathy AT yukasuzuki involvementofoxidativemodificationofproteinsrelatedtoatpsynthesisintheleftventriclesofhamsterswithcardiomyopathy AT jiechang involvementofoxidativemodificationofproteinsrelatedtoatpsynthesisintheleftventriclesofhamsterswithcardiomyopathy AT kentarokuzuya involvementofoxidativemodificationofproteinsrelatedtoatpsynthesisintheleftventriclesofhamsterswithcardiomyopathy AT chisainoue involvementofoxidativemodificationofproteinsrelatedtoatpsynthesisintheleftventriclesofhamsterswithcardiomyopathy AT yukikitamura involvementofoxidativemodificationofproteinsrelatedtoatpsynthesisintheleftventriclesofhamsterswithcardiomyopathy AT shinjioikawa involvementofoxidativemodificationofproteinsrelatedtoatpsynthesisintheleftventriclesofhamsterswithcardiomyopathy
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Involvement of oxidative modification of proteins related to ATP synthesis in the left ventricles of hamsters with cardiomyopathy

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