Silencing <i>Autophagy-Related Gene 2</i> (<i>ATG2</i>) Results in Accelerated Senescence and Enhanced Immunity in Soybean
Autophagy plays a critical role in nutrient recycling and stress adaptations. However, the role of autophagy has not been extensively investigated in crop plants. In this study, soybean <i>autophagy-related gene 2</i> (<i>GmATG2</i>) was silenced, using virus-induced silencin...
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| Autores principales: | , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/594c587ff2e048458c6d6f1fd4b382d8 |
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| Sumario: | Autophagy plays a critical role in nutrient recycling and stress adaptations. However, the role of autophagy has not been extensively investigated in crop plants. In this study, soybean <i>autophagy-related gene 2</i> (<i>GmATG2</i>) was silenced, using virus-induced silencing (VIGS) mediated by <i>Bean pod mottle virus</i> (BPMV). An accelerated senescence phenotype was exclusively observed for the <i>GmATG2-</i>silenced plants under dark conditions. In addition, significantly increased accumulation of both ROS and SA as well as a significantly induced expression of the pathogenesis-related gene 1 (<i>PR1</i>) were also observed on the leaves of the <i>GmATG2-</i>silenced plants, indicating an activated immune response. Consistent with this, <i>GmATG2</i>-silenced plants exhibited a significantly enhanced resistance to <i>Pseudomonas syringae</i> pv. <i>glycinea</i> (Psg) relative to empty vector control plants (BPMV-0). Notably, the activated immunity of the <i>GmATG2-</i>silenced plants was independent of the MAPK signaling pathway. The fact that the accumulation levels of ATG8 protein and poly-ubiquitinated proteins were significantly increased in the dark-treated <i>GmATG2-</i>silenced plants relative to the BPMV-0 plants indicated that the autophagic degradation is compromised in the <i>GmATG2-</i>silenced plants. Together, our results indicated that silencing <i>GmATG2</i> compromises the autophagy pathway, and the autophagy pathway is conserved in different plant species. |
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