spawning method affects the reproductive efficiency of piracanjuba fish (Brycon orbignyanus) in restocking programs

Background: Preserving the genetic diversity of wild fish is an important consideration for restocking programs, as inbreeding can compromise progeny survival as well as impact the resilience of natural populations. Objective: To evaluate the influence of spawning method: semi-natural (SN) or strip-...

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Autores principales:	Pedro L. de Castro, Nelson M. Lopera-Barrero, Elenice S. dos Reis-Goes, Felipe P. de Souza, Satia C. Bomfim, André L. Julien-Ferraz, Ricardo P. Ribeiro
Formato:	article
Lenguaje:	EN
Publicado:	Universidad de Antioquia 2020
Materias:	aquaculture breeders broodfish broodstock brycon orbignyanus fertilization fish genetic diversity hatching microsatellite markers mortality reproductive efficiency restocking spawning strip-spawning Animal culture SF1-1100
Acceso en línea:	https://doaj.org/article/595114f54a574c44b985c30b6b565aed
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spelling	oai:doaj.org-article:595114f54a574c44b985c30b6b565aed2021-12-01T15:14:34Zspawning method affects the reproductive efficiency of piracanjuba fish (Brycon orbignyanus) in restocking programs2256-295810.17533/udea.rccp.v34n1a03https://doaj.org/article/595114f54a574c44b985c30b6b565aed2020-06-01T00:00:00Zhttps://revistas.udea.edu.co/index.php/rccp/article/view/342498https://doaj.org/toc/2256-2958Background: Preserving the genetic diversity of wild fish is an important consideration for restocking programs, as inbreeding can compromise progeny survival as well as impact the resilience of natural populations. Objective: To evaluate the influence of spawning method: semi-natural (SN) or strip-spawning (ST), and the number of breeders (1♀:3♂ and 2♀:6♂) on the reproductive efficiency and genetic diversity of B. orbignyanus progeny destined for restoration of wild stocks. Methods: Rates of fertilization, hatching and broodfish mortality were recorded. For genetic evaluations (allele frequency, observed and expected heterozygosity, Shannon index, inbreeding coefficient, molecular variance analysis, and genetic differentiation), breeders (n=24), and their progenies (90 larvae/treatment) were sampled and analyzed using eight microsatellite markers. Results: Higher fertilization and hatching rates, and lower broodfish mortality were observed for the SN method (p<0.05), whereas the number of breeders did not affect these parameters (p>0.05). Interaction between spawning method and number of breeders was not significant (p>0.05). The amplified microsatellite loci produced a total of 30 alleles, with sizes between 80 and 225 bp and their frequencies indicated an increase (p<0.05) of genetic diversity in the progenies, but low genetic differentiation between treatments (p>0.05). Conclusion: The spawning methods and number of breeders tested increased equally the genetic diversity of the progeny, with low genetic differentiation between treatments. In contrast, rates of fertilization, hatching and brood fish mortality revealed that the SN method resulted in the best reproductive efficiency due to the handling stress and injuries caused by ST. Thus, SN proves to be the most suitable spawning-method for B. orbignyanus in restocking programs.Pedro L. de CastroNelson M. Lopera-BarreroElenice S. dos Reis-GoesFelipe P. de SouzaSatia C. BomfimAndré L. Julien-FerrazRicardo P. RibeiroUniversidad de Antioquiaarticleaquaculturebreedersbroodfishbroodstockbrycon orbignyanusfertilizationfishgenetic diversityhatchingmicrosatellite markersmortalityreproductive efficiencyrestockingspawningstrip-spawningAnimal cultureSF1-1100ENRevista Colombiana de Ciencias Pecuarias, Vol 34, Iss 1, Pp 29-39 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	aquaculture breeders broodfish broodstock brycon orbignyanus fertilization fish genetic diversity hatching microsatellite markers mortality reproductive efficiency restocking spawning strip-spawning Animal culture SF1-1100
spellingShingle	aquaculture breeders broodfish broodstock brycon orbignyanus fertilization fish genetic diversity hatching microsatellite markers mortality reproductive efficiency restocking spawning strip-spawning Animal culture SF1-1100 Pedro L. de Castro Nelson M. Lopera-Barrero Elenice S. dos Reis-Goes Felipe P. de Souza Satia C. Bomfim André L. Julien-Ferraz Ricardo P. Ribeiro spawning method affects the reproductive efficiency of piracanjuba fish (Brycon orbignyanus) in restocking programs
description	Background: Preserving the genetic diversity of wild fish is an important consideration for restocking programs, as inbreeding can compromise progeny survival as well as impact the resilience of natural populations. Objective: To evaluate the influence of spawning method: semi-natural (SN) or strip-spawning (ST), and the number of breeders (1♀:3♂ and 2♀:6♂) on the reproductive efficiency and genetic diversity of B. orbignyanus progeny destined for restoration of wild stocks. Methods: Rates of fertilization, hatching and broodfish mortality were recorded. For genetic evaluations (allele frequency, observed and expected heterozygosity, Shannon index, inbreeding coefficient, molecular variance analysis, and genetic differentiation), breeders (n=24), and their progenies (90 larvae/treatment) were sampled and analyzed using eight microsatellite markers. Results: Higher fertilization and hatching rates, and lower broodfish mortality were observed for the SN method (p<0.05), whereas the number of breeders did not affect these parameters (p>0.05). Interaction between spawning method and number of breeders was not significant (p>0.05). The amplified microsatellite loci produced a total of 30 alleles, with sizes between 80 and 225 bp and their frequencies indicated an increase (p<0.05) of genetic diversity in the progenies, but low genetic differentiation between treatments (p>0.05). Conclusion: The spawning methods and number of breeders tested increased equally the genetic diversity of the progeny, with low genetic differentiation between treatments. In contrast, rates of fertilization, hatching and brood fish mortality revealed that the SN method resulted in the best reproductive efficiency due to the handling stress and injuries caused by ST. Thus, SN proves to be the most suitable spawning-method for B. orbignyanus in restocking programs.
format	article
author	Pedro L. de Castro Nelson M. Lopera-Barrero Elenice S. dos Reis-Goes Felipe P. de Souza Satia C. Bomfim André L. Julien-Ferraz Ricardo P. Ribeiro
author_facet	Pedro L. de Castro Nelson M. Lopera-Barrero Elenice S. dos Reis-Goes Felipe P. de Souza Satia C. Bomfim André L. Julien-Ferraz Ricardo P. Ribeiro
author_sort	Pedro L. de Castro
title	spawning method affects the reproductive efficiency of piracanjuba fish (Brycon orbignyanus) in restocking programs
title_short	spawning method affects the reproductive efficiency of piracanjuba fish (Brycon orbignyanus) in restocking programs
title_full	spawning method affects the reproductive efficiency of piracanjuba fish (Brycon orbignyanus) in restocking programs
title_fullStr	spawning method affects the reproductive efficiency of piracanjuba fish (Brycon orbignyanus) in restocking programs
title_full_unstemmed	spawning method affects the reproductive efficiency of piracanjuba fish (Brycon orbignyanus) in restocking programs
title_sort	spawning method affects the reproductive efficiency of piracanjuba fish (brycon orbignyanus) in restocking programs
publisher	Universidad de Antioquia
publishDate	2020
url	https://doaj.org/article/595114f54a574c44b985c30b6b565aed
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spawning method affects the reproductive efficiency of piracanjuba fish (Brycon orbignyanus) in restocking programs

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