Tracking Recombination Events That Occur in Conjugative Virulence Plasmid p15WZ-82_Vir during the Transmission Process

ABSTRACT We recently reported the recovery of a conjugative virulence plasmid, p15WZ-82_Vir, from a clinical Klebsiella variicola strain. In this study, we found that several new plasmid types were generated due to genetic rearrangement. Partial integration of plasmid p15WZ-82_Vir with existing plas...

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Autores principales:	Xuemei Yang, Lianwei Ye, Edward Wai-Chi Chan, Rong Zhang, Sheng Chen
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	Klebsiella pneumoniae hypervirulence plasmid conjugative plasmid evolution homologous recombination Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/595366384646434bbae58f89ded0daa0
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spelling	oai:doaj.org-article:595366384646434bbae58f89ded0daa02021-12-02T18:15:46ZTracking Recombination Events That Occur in Conjugative Virulence Plasmid p15WZ-82_Vir during the Transmission Process10.1128/mSystems.00140-202379-5077https://doaj.org/article/595366384646434bbae58f89ded0daa02020-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00140-20https://doaj.org/toc/2379-5077ABSTRACT We recently reported the recovery of a conjugative virulence plasmid, p15WZ-82_Vir, from a clinical Klebsiella variicola strain. In this study, we found that several new plasmid types were generated due to genetic rearrangement. Partial integration of plasmid p15WZ-82_Vir with existing plasmids such as resistance plasmids by different homologous recombination events was observable in three recipient strains. Such recombination events enable the formation of various types of mosaic plasmids simultaneously carrying virulence-encoding and antibiotic resistance-encoding genes as well as genes involved in plasmid conjugation, which promote transmission of various virulence-encoding and resistance-encoding elements among pathogens. Our data also suggest that these conjugative events may play an integral role in the development of novel mosaic plasmids, which is vital for plasmid evolution. IMPORTANCE Although they are often nonconjugative, large virulence plasmids are increasingly detected in clinical K. pneumoniae and contribute to the hypervirulence phenotype of this organism. In this study, we demonstrated that the virulence-encoding region that originated from virulence plasmid pLVPK actively interacted with different types of plasmids via homologous recombination to generate new conjugative plasmids. This report provides insights into the evolution of self-transmissible plasmids carrying genetic elements encoding both hypervirulent and multidrug-resistant phenotypes, which facilitate the rapid development of clinical K. pneumoniae strains that are hypervirulent and multidrug resistant.Xuemei YangLianwei YeEdward Wai-Chi ChanRong ZhangSheng ChenAmerican Society for MicrobiologyarticleKlebsiella pneumoniaehypervirulence plasmidconjugativeplasmid evolutionhomologous recombinationMicrobiologyQR1-502ENmSystems, Vol 5, Iss 4 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Klebsiella pneumoniae hypervirulence plasmid conjugative plasmid evolution homologous recombination Microbiology QR1-502
spellingShingle	Klebsiella pneumoniae hypervirulence plasmid conjugative plasmid evolution homologous recombination Microbiology QR1-502 Xuemei Yang Lianwei Ye Edward Wai-Chi Chan Rong Zhang Sheng Chen Tracking Recombination Events That Occur in Conjugative Virulence Plasmid p15WZ-82_Vir during the Transmission Process
description	ABSTRACT We recently reported the recovery of a conjugative virulence plasmid, p15WZ-82_Vir, from a clinical Klebsiella variicola strain. In this study, we found that several new plasmid types were generated due to genetic rearrangement. Partial integration of plasmid p15WZ-82_Vir with existing plasmids such as resistance plasmids by different homologous recombination events was observable in three recipient strains. Such recombination events enable the formation of various types of mosaic plasmids simultaneously carrying virulence-encoding and antibiotic resistance-encoding genes as well as genes involved in plasmid conjugation, which promote transmission of various virulence-encoding and resistance-encoding elements among pathogens. Our data also suggest that these conjugative events may play an integral role in the development of novel mosaic plasmids, which is vital for plasmid evolution. IMPORTANCE Although they are often nonconjugative, large virulence plasmids are increasingly detected in clinical K. pneumoniae and contribute to the hypervirulence phenotype of this organism. In this study, we demonstrated that the virulence-encoding region that originated from virulence plasmid pLVPK actively interacted with different types of plasmids via homologous recombination to generate new conjugative plasmids. This report provides insights into the evolution of self-transmissible plasmids carrying genetic elements encoding both hypervirulent and multidrug-resistant phenotypes, which facilitate the rapid development of clinical K. pneumoniae strains that are hypervirulent and multidrug resistant.
format	article
author	Xuemei Yang Lianwei Ye Edward Wai-Chi Chan Rong Zhang Sheng Chen
author_facet	Xuemei Yang Lianwei Ye Edward Wai-Chi Chan Rong Zhang Sheng Chen
author_sort	Xuemei Yang
title	Tracking Recombination Events That Occur in Conjugative Virulence Plasmid p15WZ-82_Vir during the Transmission Process
title_short	Tracking Recombination Events That Occur in Conjugative Virulence Plasmid p15WZ-82_Vir during the Transmission Process
title_full	Tracking Recombination Events That Occur in Conjugative Virulence Plasmid p15WZ-82_Vir during the Transmission Process
title_fullStr	Tracking Recombination Events That Occur in Conjugative Virulence Plasmid p15WZ-82_Vir during the Transmission Process
title_full_unstemmed	Tracking Recombination Events That Occur in Conjugative Virulence Plasmid p15WZ-82_Vir during the Transmission Process
title_sort	tracking recombination events that occur in conjugative virulence plasmid p15wz-82_vir during the transmission process
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/595366384646434bbae58f89ded0daa0
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Tracking Recombination Events That Occur in Conjugative Virulence Plasmid p15WZ-82_Vir during the Transmission Process

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