Relationship between aqueous humor cytokine level changes and retinal vascular changes after intravitreal aflibercept for diabetic macular edema

Abstract The aim of this work was to investigate the changes in aqueous humor cytokine levels after intravitreal injection of aflibercept in diabetic macular edema (DME) and to evaluate the relationship between cytokines modifications and central macular thickness (CMT) and retinal/choroidal vascula...

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Autores principales:	Rodolfo Mastropasqua, Rossella D’Aloisio, Marta Di Nicola, Giuseppe Di Martino, Alessia Lamolinara, Luca Di Antonio, Daniele Tognetto, Lisa Toto
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Aflibercept Diabetic Macular Edema (DME) Central Macular Thickness (CMT) Vascular Endothelial Growth Factor (VEGF) Superficial Capillary Plexus (SCP) Medicine R Science Q
Acceso en línea:	https://doaj.org/article/595803eecb934c5cbfe82fd32fce1dae
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spelling	oai:doaj.org-article:595803eecb934c5cbfe82fd32fce1dae2021-12-02T15:07:52ZRelationship between aqueous humor cytokine level changes and retinal vascular changes after intravitreal aflibercept for diabetic macular edema10.1038/s41598-018-35036-92045-2322https://doaj.org/article/595803eecb934c5cbfe82fd32fce1dae2018-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-35036-9https://doaj.org/toc/2045-2322Abstract The aim of this work was to investigate the changes in aqueous humor cytokine levels after intravitreal injection of aflibercept in diabetic macular edema (DME) and to evaluate the relationship between cytokines modifications and central macular thickness (CMT) and retinal/choroidal vascular changes using structural and functional optical coherence tomography (OCT). Aqueous concentrations of 38 cytokines were measured via multiplex bead assay. In addition, spectral domain OCT and OCT angiography with SSADA software (XR Avanti® AngioVue) were performed at baseline and after intravitreal injections. VEGF, IL-6, IL-5, IL-1β, Eotaxin, GRO, IL-12p40, IL-12p70, IL-1RA, Flt-3L and IP-10 showed a statistically significant decrease through the follow-up (p < 0.05; p < 0.001), while Fraktalkine and GM-CSF significantly increased (p < 0.05). Best corrected visual acuity significantly increased and CMT significantly decreased during follow-up (p < 0.001 and p = 0.013). Superficial capillary plexus and deep capillary plexus density significantly increased (p < 0.001 and p = 0.014). A positive relation was found between GRO, VEGF, Fraktalkine, IP-10, IL-12p70 aqueous humor levels and CMT (p < 0.05; p < 0.001). Aflibercept is a primary anti-VEGF treatment producing a decrease of DME due to the reduction of vascular permeability, nevertheless other inflammatory cytokines showed modification after aflibercept intravitreal injections probably related to edema modification or to an interaction of aflibercept with other inflammatory cytokines.Rodolfo MastropasquaRossella D’AloisioMarta Di NicolaGiuseppe Di MartinoAlessia LamolinaraLuca Di AntonioDaniele TognettoLisa TotoNature PortfolioarticleAfliberceptDiabetic Macular Edema (DME)Central Macular Thickness (CMT)Vascular Endothelial Growth Factor (VEGF)Superficial Capillary Plexus (SCP)MedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Aflibercept Diabetic Macular Edema (DME) Central Macular Thickness (CMT) Vascular Endothelial Growth Factor (VEGF) Superficial Capillary Plexus (SCP) Medicine R Science Q
spellingShingle	Aflibercept Diabetic Macular Edema (DME) Central Macular Thickness (CMT) Vascular Endothelial Growth Factor (VEGF) Superficial Capillary Plexus (SCP) Medicine R Science Q Rodolfo Mastropasqua Rossella D’Aloisio Marta Di Nicola Giuseppe Di Martino Alessia Lamolinara Luca Di Antonio Daniele Tognetto Lisa Toto Relationship between aqueous humor cytokine level changes and retinal vascular changes after intravitreal aflibercept for diabetic macular edema
description	Abstract The aim of this work was to investigate the changes in aqueous humor cytokine levels after intravitreal injection of aflibercept in diabetic macular edema (DME) and to evaluate the relationship between cytokines modifications and central macular thickness (CMT) and retinal/choroidal vascular changes using structural and functional optical coherence tomography (OCT). Aqueous concentrations of 38 cytokines were measured via multiplex bead assay. In addition, spectral domain OCT and OCT angiography with SSADA software (XR Avanti® AngioVue) were performed at baseline and after intravitreal injections. VEGF, IL-6, IL-5, IL-1β, Eotaxin, GRO, IL-12p40, IL-12p70, IL-1RA, Flt-3L and IP-10 showed a statistically significant decrease through the follow-up (p < 0.05; p < 0.001), while Fraktalkine and GM-CSF significantly increased (p < 0.05). Best corrected visual acuity significantly increased and CMT significantly decreased during follow-up (p < 0.001 and p = 0.013). Superficial capillary plexus and deep capillary plexus density significantly increased (p < 0.001 and p = 0.014). A positive relation was found between GRO, VEGF, Fraktalkine, IP-10, IL-12p70 aqueous humor levels and CMT (p < 0.05; p < 0.001). Aflibercept is a primary anti-VEGF treatment producing a decrease of DME due to the reduction of vascular permeability, nevertheless other inflammatory cytokines showed modification after aflibercept intravitreal injections probably related to edema modification or to an interaction of aflibercept with other inflammatory cytokines.
format	article
author	Rodolfo Mastropasqua Rossella D’Aloisio Marta Di Nicola Giuseppe Di Martino Alessia Lamolinara Luca Di Antonio Daniele Tognetto Lisa Toto
author_facet	Rodolfo Mastropasqua Rossella D’Aloisio Marta Di Nicola Giuseppe Di Martino Alessia Lamolinara Luca Di Antonio Daniele Tognetto Lisa Toto
author_sort	Rodolfo Mastropasqua
title	Relationship between aqueous humor cytokine level changes and retinal vascular changes after intravitreal aflibercept for diabetic macular edema
title_short	Relationship between aqueous humor cytokine level changes and retinal vascular changes after intravitreal aflibercept for diabetic macular edema
title_full	Relationship between aqueous humor cytokine level changes and retinal vascular changes after intravitreal aflibercept for diabetic macular edema
title_fullStr	Relationship between aqueous humor cytokine level changes and retinal vascular changes after intravitreal aflibercept for diabetic macular edema
title_full_unstemmed	Relationship between aqueous humor cytokine level changes and retinal vascular changes after intravitreal aflibercept for diabetic macular edema
title_sort	relationship between aqueous humor cytokine level changes and retinal vascular changes after intravitreal aflibercept for diabetic macular edema
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/595803eecb934c5cbfe82fd32fce1dae
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Relationship between aqueous humor cytokine level changes and retinal vascular changes after intravitreal aflibercept for diabetic macular edema

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